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The map label for this gene is nifS [H]

Identifier: 46446355

GI number: 46446355

Start: 876188

End: 877357

Strand: Direct

Name: nifS [H]

Synonym: pc0721

Alternate gene names: 46446355

Gene position: 876188-877357 (Clockwise)

Preceding gene: 46446354

Following gene: 46446358

Centisome position: 36.29

GC content: 39.23

Gene sequence:

>1170_bases
TTGTTGTTATTAAAGTTCAGGGAAAATATGAATCGGCGAGTCTATCTTGATTGCAATTCTAGCACCGCGATCGATCCTCG
CGTTTTGAAAGTTCTGATCCAAGAACTTCAAGAAGAAGAGGGAAATCCTTCAAGCGTTCATTATCACGGGCAGTTCTGTC
GCAAAAAATTAGAAGAAAGTCGTCAAATAATTGCTCGCTTTCTAAAAGTCAAACCACAAGAAATTATTTTCACTTCAGGC
GGAACAGAAGGAGCTAATCTTCTTATTAAGGGAGTTCTTCAACATTTTTCTTTGGGGCAGATCATTTCATCAAATGTCGA
ACATGCCTGCGTTGATGAAGTTTTGAAAAAAATGGAAAAACAAGGACATTCTGTCACGTTCCTTGAAACAGGTTCATGGG
GTGCTGTAGATCCACAAAAACTAAAAGAAGCTATTGGATCTCAAACGCGACTCATTACTTTAATGGCTGCTAATAACGAA
ACAGGCGTAAAAACAGATATAGAAGCTTGTGCTGCGATTGCTCAAGAGGCTCAAATCCCTTTTATTGTAGATGGGGTTGC
CTTACTCGGAAAAGAAATGTTTAATATTCCTAAAGGGGTATCAGCCATGTTTTTTAGTGGCCACAAAGTGCATGTCCCTA
AAGGAATCGGTTTTTGTTTTGTTCGACAAGGGTTAAAATTAATGCCTCAGATAATAGGAGGGGGCCAAGAGCAAAATCGT
CGATCTGGAACAGAGAATTTAGCAGGTATTGTTGCTTTAGCAGAAGGCATTTCTATTTTATCGGAAAGCCAAGAAAAGAT
TACTAATCAATTACGCTCAAGACGAGATCAATTAGAAAAGGGATTGATCAATCAATTAGATCAGGTGATCATTAATGGCG
ATGCTCCCCGTATCGCCAACACTTCTAACTTATCATTTGTTGGTATTGATGGAGAAACACTCTTGATGCAGTTAGACATG
CAAGGGATTTCTGTGAGTCATGGATCTGCTTGCTCATCAGGAGCTCTTGAACCCTCCCGTGTCCTGTTGAATATGGGTTT
TTCTAATAAACGTGCAGGATCTGCGATTCGCTTTTCTGTGGGCCGTATGACAACAGAGGAGGATATTAAACAAACGATTC
AAGTTCTTGTTAAAACTATCACGAAATTTAAATCCAATTCTTTTACTTAG

Upstream 100 bases:

>100_bases
CAAGACATTCAACAAATTTTATCAGATACTCCTGATGATGAAGTTACCAAAAAATTGGTAAAAGTGGCGAAATCGAGAGG
AGGACACGATAACATCACAG

Downstream 100 bases:

>100_bases
ACATGTTGAGGCATTGAAACAGGAATTTAGTGTGTTTGATTATCGGAAGGATAAAAAAAAACTTTTTTCTTTCCTTCAGG
AACAAGGCCTAACCCCTTCA

Product: putative cysteine sulfinate desulfinase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLLLKFRENMNRRVYLDCNSSTAIDPRVLKVLIQELQEEEGNPSSVHYHGQFCRKKLEESRQIIARFLKVKPQEIIFTSG
GTEGANLLIKGVLQHFSLGQIISSNVEHACVDEVLKKMEKQGHSVTFLETGSWGAVDPQKLKEAIGSQTRLITLMAANNE
TGVKTDIEACAAIAQEAQIPFIVDGVALLGKEMFNIPKGVSAMFFSGHKVHVPKGIGFCFVRQGLKLMPQIIGGGQEQNR
RSGTENLAGIVALAEGISILSESQEKITNQLRSRRDQLEKGLINQLDQVIINGDAPRIANTSNLSFVGIDGETLLMQLDM
QGISVSHGSACSSGALEPSRVLLNMGFSNKRAGSAIRFSVGRMTTEEDIKQTIQVLVKTITKFKSNSFT

Sequences:

>Translated_389_residues
MLLLKFRENMNRRVYLDCNSSTAIDPRVLKVLIQELQEEEGNPSSVHYHGQFCRKKLEESRQIIARFLKVKPQEIIFTSG
GTEGANLLIKGVLQHFSLGQIISSNVEHACVDEVLKKMEKQGHSVTFLETGSWGAVDPQKLKEAIGSQTRLITLMAANNE
TGVKTDIEACAAIAQEAQIPFIVDGVALLGKEMFNIPKGVSAMFFSGHKVHVPKGIGFCFVRQGLKLMPQIIGGGQEQNR
RSGTENLAGIVALAEGISILSESQEKITNQLRSRRDQLEKGLINQLDQVIINGDAPRIANTSNLSFVGIDGETLLMQLDM
QGISVSHGSACSSGALEPSRVLLNMGFSNKRAGSAIRFSVGRMTTEEDIKQTIQVLVKTITKFKSNSFT
>Mature_389_residues
MLLLKFRENMNRRVYLDCNSSTAIDPRVLKVLIQELQEEEGNPSSVHYHGQFCRKKLEESRQIIARFLKVKPQEIIFTSG
GTEGANLLIKGVLQHFSLGQIISSNVEHACVDEVLKKMEKQGHSVTFLETGSWGAVDPQKLKEAIGSQTRLITLMAANNE
TGVKTDIEACAAIAQEAQIPFIVDGVALLGKEMFNIPKGVSAMFFSGHKVHVPKGIGFCFVRQGLKLMPQIIGGGQEQNR
RSGTENLAGIVALAEGISILSESQEKITNQLRSRRDQLEKGLINQLDQVIINGDAPRIANTSNLSFVGIDGETLLMQLDM
QGISVSHGSACSSGALEPSRVLLNMGFSNKRAGSAIRFSVGRMTTEEDIKQTIQVLVKTITKFKSNSFT

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=33.6814621409922, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI156713448, Length=420, Percent_Identity=32.8571428571429, Blast_Score=188, Evalue=8e-48,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=37.2093023255814, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1789175, Length=276, Percent_Identity=24.6376811594203, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25143064, Length=382, Percent_Identity=34.0314136125654, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=32.1766561514196, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=381, Percent_Identity=35.1706036745407, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI20129463, Length=395, Percent_Identity=34.6835443037975, Blast_Score=225, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42612; Mature: 42612

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLKFRENMNRRVYLDCNSSTAIDPRVLKVLIQELQEEEGNPSSVHYHGQFCRKKLEES
CEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
RQIIARFLKVKPQEIIFTSGGTEGANLLIKGVLQHFSLGQIISSNVEHACVDEVLKKMEK
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QGHSVTFLETGSWGAVDPQKLKEAIGSQTRLITLMAANNETGVKTDIEACAAIAQEAQIP
CCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
FIVDGVALLGKEMFNIPKGVSAMFFSGHKVHVPKGIGFCFVRQGLKLMPQIIGGGQEQNR
EEEHHHHHHHHHHHCCCCCCHHHEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHH
RSGTENLAGIVALAEGISILSESQEKITNQLRSRRDQLEKGLINQLDQVIINGDAPRIAN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
TSNLSFVGIDGETLLMQLDMQGISVSHGSACSSGALEPSRVLLNMGFSNKRAGSAIRFSV
CCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCEEEEEC
GRMTTEEDIKQTIQVLVKTITKFKSNSFT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLLKFRENMNRRVYLDCNSSTAIDPRVLKVLIQELQEEEGNPSSVHYHGQFCRKKLEES
CEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
RQIIARFLKVKPQEIIFTSGGTEGANLLIKGVLQHFSLGQIISSNVEHACVDEVLKKMEK
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QGHSVTFLETGSWGAVDPQKLKEAIGSQTRLITLMAANNETGVKTDIEACAAIAQEAQIP
CCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
FIVDGVALLGKEMFNIPKGVSAMFFSGHKVHVPKGIGFCFVRQGLKLMPQIIGGGQEQNR
EEEHHHHHHHHHHHCCCCCCHHHEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHH
RSGTENLAGIVALAEGISILSESQEKITNQLRSRRDQLEKGLINQLDQVIINGDAPRIAN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
TSNLSFVGIDGETLLMQLDMQGISVSHGSACSSGALEPSRVLLNMGFSNKRAGSAIRFSV
CCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCEEEEEC
GRMTTEEDIKQTIQVLVKTITKFKSNSFT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]