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The map label for this gene is mazG [H]

Identifier: 46446351

GI number: 46446351

Start: 871260

End: 871913

Strand: Direct

Name: mazG [H]

Synonym: pc0717

Alternate gene names: 46446351

Gene position: 871260-871913 (Clockwise)

Preceding gene: 46446341

Following gene: 46446352

Centisome position: 36.09

GC content: 35.93

Gene sequence:

>654_bases
ATGGATGATTTTAAAGCTCTTATTATCACCATTGAACGTTTGTTAGCCCCAAATGGTTGTCCATGGGATCGAGAGCAAAC
GCTTCAATCGATGCGAAGTTCAGTCATTGAAGAAGTCTACGAACTAGTAGAAGCCATCGATCTTGAAAACAATGAAAAAA
TTAAAGAGGAGCTTGGTGATCTTTGTTTTAATGTGATTTTTTTAGGAAAACTGGCTGAACGAGACAAAAAATTTTCTTTG
CATGATATGTTGAAAGAGATTAATGAAAAACTGATTAGGCGGCATCCTCATATTTTTGAAGAAATTCAGCTTGATTCAGT
TGCACAAGTGAAGCATCAATGGGAAATAATTAAGAAAAATGAAAAAGGTAATCGGTGGAGACAGAGTGTGTTGGACGGGA
TTCCTAAAGATTTCCCTTCATTGGCGCGGGCAGAGAAAATGATTAAAGAATTTGAAAAAACCGATTTTGCATGGCAAAAT
CGAGAACATTTTTCTTCCCAAGAGGACCTATTTGCGCAAGACTTATTAAATCTAATTAAAAAGGCCTCTCAAAATGGAAT
AAAGGTAGAAACAACACTTCGTAAAATGCTATCTCAATTAGAACAACAATTTCGTCAGTGGGAAAATGCTCAAAAACGTG
TAGAATCTGCTTGA

Upstream 100 bases:

>100_bases
AAATCCAAATTCAAATGATTCTAATTTTAAAAAAATATTTCTGCAATTGATGTTAACGAATTGTTGATTTTTTCGTCCTA
TTATTTAAAGGAGAAACCAA

Downstream 100 bases:

>100_bases
TAGAAAACACAACTCGTTATAAAATGACATTTTCTTAGAGTTTACCGATAAATAAAGTTTAACCGGGGACCTCATGGAAT
AAATTAGTTCGAACCAGGTC

Product: hypothetical protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MDDFKALIITIERLLAPNGCPWDREQTLQSMRSSVIEEVYELVEAIDLENNEKIKEELGDLCFNVIFLGKLAERDKKFSL
HDMLKEINEKLIRRHPHIFEEIQLDSVAQVKHQWEIIKKNEKGNRWRQSVLDGIPKDFPSLARAEKMIKEFEKTDFAWQN
REHFSSQEDLFAQDLLNLIKKASQNGIKVETTLRKMLSQLEQQFRQWENAQKRVESA

Sequences:

>Translated_217_residues
MDDFKALIITIERLLAPNGCPWDREQTLQSMRSSVIEEVYELVEAIDLENNEKIKEELGDLCFNVIFLGKLAERDKKFSL
HDMLKEINEKLIRRHPHIFEEIQLDSVAQVKHQWEIIKKNEKGNRWRQSVLDGIPKDFPSLARAEKMIKEFEKTDFAWQN
REHFSSQEDLFAQDLLNLIKKASQNGIKVETTLRKMLSQLEQQFRQWENAQKRVESA
>Mature_217_residues
MDDFKALIITIERLLAPNGCPWDREQTLQSMRSSVIEEVYELVEAIDLENNEKIKEELGDLCFNVIFLGKLAERDKKFSL
HDMLKEINEKLIRRHPHIFEEIQLDSVAQVKHQWEIIKKNEKGNRWRQSVLDGIPKDFPSLARAEKMIKEFEKTDFAWQN
REHFSSQEDLFAQDLLNLIKKASQNGIKVETTLRKMLSQLEQQFRQWENAQKRVESA

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=231, Percent_Identity=32.034632034632, Blast_Score=133, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 25682; Mature: 25682

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDFKALIITIERLLAPNGCPWDREQTLQSMRSSVIEEVYELVEAIDLENNEKIKEELGD
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LCFNVIFLGKLAERDKKFSLHDMLKEINEKLIRRHPHIFEEIQLDSVAQVKHQWEIIKKN
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
EKGNRWRQSVLDGIPKDFPSLARAEKMIKEFEKTDFAWQNREHFSSQEDLFAQDLLNLIK
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCHHHCCCHHHHHHHHHHHHHH
KASQNGIKVETTLRKMLSQLEQQFRQWENAQKRVESA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDDFKALIITIERLLAPNGCPWDREQTLQSMRSSVIEEVYELVEAIDLENNEKIKEELGD
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LCFNVIFLGKLAERDKKFSLHDMLKEINEKLIRRHPHIFEEIQLDSVAQVKHQWEIIKKN
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
EKGNRWRQSVLDGIPKDFPSLARAEKMIKEFEKTDFAWQNREHFSSQEDLFAQDLLNLIK
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCHHHCCCHHHHHHHHHHHHHH
KASQNGIKVETTLRKMLSQLEQQFRQWENAQKRVESA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]