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The map label for this gene is metG

Identifier: 46446299

GI number: 46446299

Start: 808477

End: 810573

Strand: Direct

Name: metG

Synonym: pc0665

Alternate gene names: 46446299

Gene position: 808477-810573 (Clockwise)

Preceding gene: 46446298

Following gene: 46446306

Centisome position: 33.48

GC content: 36.86

Gene sequence:

>2097_bases
TTGTTCAAAAACCTGTGCGATAAGATAATGACCCAAAAAATTTTGATTACTTCTGCTCTTCCCTATGCGAATGGACCTCT
GCATTTTGGACATATTGCAGGGGCTTACCTCCCAGCTGACTGTTATGCACGTTTTCAAAGATTAATGAAAAAAGATGTTC
TTTACATTTGTGGATCCGATGAATATGGCATTGCGATCACCTTAAGTGCTGATTTAGCTGGTAGAACACCTCAAGAACAT
GTTGATCTTTTTCATCACATTAATCAAAGTTTTTTCGAGCAACTCCAAATTTCTTTTGATCATTATTCTCGTACGACCTG
GAAAGGACATGTAGAGCCTACCCATCAGTTCTTTAATGACTTATTGCAGAATGGATATATTGAAGAACGCACGACCGACC
AACTCTATTCTGAAAAAGATCAGAAATTTCTTGCAGATCGTTATGTCGTGGGAACGTGTCCTCGTTGTGGTTTTGAAAAT
GCAAGGGGGGATGAGTGTCCTTGTTGTGGTGCAAGCTATGAAGCTACAGAACTTAAAAACCCTCGTTCTAAACTCACAGA
TGCTTCCTTGATTTTGCGACCGACTAAACACTGGTTTTTACTTCTTGAAAAATTTAAAAAACCTTTAATGGAGTGGTTGG
AAACTAAAAACTGGAAGCCAAATGTAATTAATTTTATTAGGGGATACATCGATCATCTCCATGCCAGAGCAATCACAAGA
GATATGAAATGGGGAATTTCTGTTCCTCTTCCTGATTCAGAAGGAAAAGTCCTTTATGTCTGGTTTGATGCACCTATAGG
CTATATTTCGGCGACTAAAGAGTGGGCTTTATTAAGAGGGGAAGAAAAGCTTTGGGAAAAATATTGGTTGGATCCAGAAA
CTAAGCTTGTCAATTTTATTGGAAAAGATAATATCCCTTTTCATGCCTCCATTTTCCCTGCTATGATCATGGGACAAAAT
CAGCCTTATAAACTAGTGGACGAACTGCCAGCCAATGAATTTTATAATTTAGAAGGGAAGCAATTTAGTAAATCAGATGG
ATGGTATATTGATTTAGAAGACTTTTTCAAGCATTATACCAGCGATCAAATTCGCTATGCTATTGCTTCCAACGCTCCTG
AAACATCTGATAGTGAATTTACTTGGAAAGACTTTCAACTACGCTGCAACAGTGATTTGCTAGGGAAGTACGGAAATTTG
GTTAATAGGGTTTTAGTGTTCATTCACAATCAGTGTCAGGGAAAAGTTCCGGAGTCTGGAGTTTTAGAAGAACAAGATCG
AAAATTTTTAAAAAATATTCAAGATTTGGTCGATCAAGCAGCTGCCAGTTATTCGACTTTTAAAGTTCGAAAAGCGAGCC
AAATTATGATGGAGTTATCCCAATTAGGAAATGTTTATTTTGATAGTAAAAAGCCTTGGCAAGACGCTAAAAATGATTAT
ACAAAGGGCAGAATGCATACAACGCTGAATTGTTGTATGGAGTGTTTAAAGGCGCTTGCGCTGATTTCTTTTCCCATAAT
TCCCACGGCAGCTTCAAAAGTTTGGCAATTTCTTGGATTTCAAACACCGCTCGAACAAGAAGATTGGAATCGCGTTAAAA
TAGAAAAGCTTCCGCTTAACCAAAAGATTTTAACTCCCCAAATCCTTTTTCAACGGGTTGAAAATGAGGCTGTTGAAAGA
GAAATTCTTAAATTGCAACAGTTATCTCTTACAAAAGAAAAAAAATCTACACCACAATCTACTCAGATATCTATTTCAGA
AAATATTCTTCTTAAACCATTAGTAGATATTGAAGATTTTCGAAAATTAGATTTAAGAGTCGGCAAGATCATCCAGGCAA
ATCCTGTACCGAAAAGTAAAAAACTTTTTCAATTACTTGTAGACATTGGAATTGAGAAAAGAATAGTTGTAGCTGGAGTT
GCCGAATTTTATAAAGTAGAAGATTTGATTGGGAAAAACGTTGTAGTTGTAGCGAATTTAAAGCCTGCTAATTTGATGGG
AGTGACTAGTCAAGGAATGCTACTTGCAGCTAAGGGAGAAGGAAATTTAAAACTTATTTCTATCGAGGATGTTGCTCCTG
GAAGTCTTGTCAGTTAA

Upstream 100 bases:

>100_bases
GAATATGCTTATTCAAAACCAGGCCCTTTATATGTAGATATTCAAACGAAAGTTTTACTCGGATGGAAAGTTGTTCCTGA
CACTGAATTAGAAGTTTTAA

Downstream 100 bases:

>100_bases
ATTTTGATATTGTGATTATTCAAGTCATTTTAGAGCAATTTTTAGAAAAGGGAGTAGCGACTTTCTTTATAATTCATCAA
CTCTTACTTGTTTAAAAAGA

Product: putative methionine-tRNA ligase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MFKNLCDKIMTQKILITSALPYANGPLHFGHIAGAYLPADCYARFQRLMKKDVLYICGSDEYGIAITLSADLAGRTPQEH
VDLFHHINQSFFEQLQISFDHYSRTTWKGHVEPTHQFFNDLLQNGYIEERTTDQLYSEKDQKFLADRYVVGTCPRCGFEN
ARGDECPCCGASYEATELKNPRSKLTDASLILRPTKHWFLLLEKFKKPLMEWLETKNWKPNVINFIRGYIDHLHARAITR
DMKWGISVPLPDSEGKVLYVWFDAPIGYISATKEWALLRGEEKLWEKYWLDPETKLVNFIGKDNIPFHASIFPAMIMGQN
QPYKLVDELPANEFYNLEGKQFSKSDGWYIDLEDFFKHYTSDQIRYAIASNAPETSDSEFTWKDFQLRCNSDLLGKYGNL
VNRVLVFIHNQCQGKVPESGVLEEQDRKFLKNIQDLVDQAAASYSTFKVRKASQIMMELSQLGNVYFDSKKPWQDAKNDY
TKGRMHTTLNCCMECLKALALISFPIIPTAASKVWQFLGFQTPLEQEDWNRVKIEKLPLNQKILTPQILFQRVENEAVER
EILKLQQLSLTKEKKSTPQSTQISISENILLKPLVDIEDFRKLDLRVGKIIQANPVPKSKKLFQLLVDIGIEKRIVVAGV
AEFYKVEDLIGKNVVVVANLKPANLMGVTSQGMLLAAKGEGNLKLISIEDVAPGSLVS

Sequences:

>Translated_698_residues
MFKNLCDKIMTQKILITSALPYANGPLHFGHIAGAYLPADCYARFQRLMKKDVLYICGSDEYGIAITLSADLAGRTPQEH
VDLFHHINQSFFEQLQISFDHYSRTTWKGHVEPTHQFFNDLLQNGYIEERTTDQLYSEKDQKFLADRYVVGTCPRCGFEN
ARGDECPCCGASYEATELKNPRSKLTDASLILRPTKHWFLLLEKFKKPLMEWLETKNWKPNVINFIRGYIDHLHARAITR
DMKWGISVPLPDSEGKVLYVWFDAPIGYISATKEWALLRGEEKLWEKYWLDPETKLVNFIGKDNIPFHASIFPAMIMGQN
QPYKLVDELPANEFYNLEGKQFSKSDGWYIDLEDFFKHYTSDQIRYAIASNAPETSDSEFTWKDFQLRCNSDLLGKYGNL
VNRVLVFIHNQCQGKVPESGVLEEQDRKFLKNIQDLVDQAAASYSTFKVRKASQIMMELSQLGNVYFDSKKPWQDAKNDY
TKGRMHTTLNCCMECLKALALISFPIIPTAASKVWQFLGFQTPLEQEDWNRVKIEKLPLNQKILTPQILFQRVENEAVER
EILKLQQLSLTKEKKSTPQSTQISISENILLKPLVDIEDFRKLDLRVGKIIQANPVPKSKKLFQLLVDIGIEKRIVVAGV
AEFYKVEDLIGKNVVVVANLKPANLMGVTSQGMLLAAKGEGNLKLISIEDVAPGSLVS
>Mature_698_residues
MFKNLCDKIMTQKILITSALPYANGPLHFGHIAGAYLPADCYARFQRLMKKDVLYICGSDEYGIAITLSADLAGRTPQEH
VDLFHHINQSFFEQLQISFDHYSRTTWKGHVEPTHQFFNDLLQNGYIEERTTDQLYSEKDQKFLADRYVVGTCPRCGFEN
ARGDECPCCGASYEATELKNPRSKLTDASLILRPTKHWFLLLEKFKKPLMEWLETKNWKPNVINFIRGYIDHLHARAITR
DMKWGISVPLPDSEGKVLYVWFDAPIGYISATKEWALLRGEEKLWEKYWLDPETKLVNFIGKDNIPFHASIFPAMIMGQN
QPYKLVDELPANEFYNLEGKQFSKSDGWYIDLEDFFKHYTSDQIRYAIASNAPETSDSEFTWKDFQLRCNSDLLGKYGNL
VNRVLVFIHNQCQGKVPESGVLEEQDRKFLKNIQDLVDQAAASYSTFKVRKASQIMMELSQLGNVYFDSKKPWQDAKNDY
TKGRMHTTLNCCMECLKALALISFPIIPTAASKVWQFLGFQTPLEQEDWNRVKIEKLPLNQKILTPQILFQRVENEAVER
EILKLQQLSLTKEKKSTPQSTQISISENILLKPLVDIEDFRKLDLRVGKIIQANPVPKSKKLFQLLVDIGIEKRIVVAGV
AEFYKVEDLIGKNVVVVANLKPANLMGVTSQGMLLAAKGEGNLKLISIEDVAPGSLVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=577, Percent_Identity=36.0485268630849, Blast_Score=352, Evalue=6e-97,
Organism=Homo sapiens, GI24308436, Length=540, Percent_Identity=24.6296296296296, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI4507947, Length=95, Percent_Identity=43.1578947368421, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=679, Percent_Identity=30.3387334315169, Blast_Score=334, Evalue=1e-92,
Organism=Escherichia coli, GI1789455, Length=80, Percent_Identity=40, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=555, Percent_Identity=36.7567567567568, Blast_Score=355, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI212645096, Length=524, Percent_Identity=24.618320610687, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71992781, Length=413, Percent_Identity=23.2445520581114, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=34.7122302158273, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6321610, Length=428, Percent_Identity=22.196261682243, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24655311, Length=560, Percent_Identity=36.7857142857143, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI21358483, Length=532, Percent_Identity=24.812030075188, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24651924, Length=101, Percent_Identity=38.6138613861386, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PARUW (Q6MDG0)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007664.1
- ProteinModelPortal:   Q6MDG0
- SMR:   Q6MDG0
- STRING:   Q6MDG0
- GeneID:   2781150
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0665
- NMPDR:   fig|264201.1.peg.665
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   RASDHAY
- PhylomeDB:   Q6MDG0
- ProtClustDB:   CLSK2762217
- BioCyc:   CPRO264201:PC0665-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 79929; Mature: 79929

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKNLCDKIMTQKILITSALPYANGPLHFGHIAGAYLPADCYARFQRLMKKDVLYICGSD
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHCCEEEEECCC
EYGIAITLSADLAGRTPQEHVDLFHHINQSFFEQLQISFDHYSRTTWKGHVEPTHQFFND
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
LLQNGYIEERTTDQLYSEKDQKFLADRYVVGTCPRCGFENARGDECPCCGASYEATELKN
HHHCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCC
PRSKLTDASLILRPTKHWFLLLEKFKKPLMEWLETKNWKPNVINFIRGYIDHLHARAITR
CHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DMKWGISVPLPDSEGKVLYVWFDAPIGYISATKEWALLRGEEKLWEKYWLDPETKLVNFI
HHHCCEECCCCCCCCCEEEEEECCCCCCHHCCCHHHHHCCHHHHHHHHCCCCHHHHHHHH
GKDNIPFHASIFPAMIMGQNQPYKLVDELPANEFYNLEGKQFSKSDGWYIDLEDFFKHYT
CCCCCCEEHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCCCCCCCCCCEEEHHHHHHHCC
SDQIRYAIASNAPETSDSEFTWKDFQLRCNSDLLGKYGNLVNRVLVFIHNQCQGKVPESG
CCCEEEEEECCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VLEEQDRKFLKNIQDLVDQAAASYSTFKVRKASQIMMELSQLGNVYFDSKKPWQDAKNDY
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCC
TKGRMHTTLNCCMECLKALALISFPIIPTAASKVWQFLGFQTPLEQEDWNRVKIEKLPLN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
QKILTPQILFQRVENEAVEREILKLQQLSLTKEKKSTPQSTQISISENILLKPLVDIEDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCHHHH
RKLDLRVGKIIQANPVPKSKKLFQLLVDIGIEKRIVVAGVAEFYKVEDLIGKNVVVVANL
HHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEC
KPANLMGVTSQGMLLAAKGEGNLKLISIEDVAPGSLVS
CCCCEEEECCCCEEEEEECCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MFKNLCDKIMTQKILITSALPYANGPLHFGHIAGAYLPADCYARFQRLMKKDVLYICGSD
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHCCEEEEECCC
EYGIAITLSADLAGRTPQEHVDLFHHINQSFFEQLQISFDHYSRTTWKGHVEPTHQFFND
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
LLQNGYIEERTTDQLYSEKDQKFLADRYVVGTCPRCGFENARGDECPCCGASYEATELKN
HHHCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCC
PRSKLTDASLILRPTKHWFLLLEKFKKPLMEWLETKNWKPNVINFIRGYIDHLHARAITR
CHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DMKWGISVPLPDSEGKVLYVWFDAPIGYISATKEWALLRGEEKLWEKYWLDPETKLVNFI
HHHCCEECCCCCCCCCEEEEEECCCCCCHHCCCHHHHHCCHHHHHHHHCCCCHHHHHHHH
GKDNIPFHASIFPAMIMGQNQPYKLVDELPANEFYNLEGKQFSKSDGWYIDLEDFFKHYT
CCCCCCEEHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCCCCCCCCCCEEEHHHHHHHCC
SDQIRYAIASNAPETSDSEFTWKDFQLRCNSDLLGKYGNLVNRVLVFIHNQCQGKVPESG
CCCEEEEEECCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VLEEQDRKFLKNIQDLVDQAAASYSTFKVRKASQIMMELSQLGNVYFDSKKPWQDAKNDY
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCC
TKGRMHTTLNCCMECLKALALISFPIIPTAASKVWQFLGFQTPLEQEDWNRVKIEKLPLN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
QKILTPQILFQRVENEAVEREILKLQQLSLTKEKKSTPQSTQISISENILLKPLVDIEDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCHHHH
RKLDLRVGKIIQANPVPKSKKLFQLLVDIGIEKRIVVAGVAEFYKVEDLIGKNVVVVANL
HHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEC
KPANLMGVTSQGMLLAAKGEGNLKLISIEDVAPGSLVS
CCCCEEEECCCCEEEEEECCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA