Click here to switch to the map view.

The map label for this gene is phrB [H]

Identifier: 46446306

GI number: 46446306

Start: 816135

End: 817550

Strand: Direct

Name: phrB [H]

Synonym: pc0672

Alternate gene names: 46446306

Gene position: 816135-817550 (Clockwise)

Preceding gene: 46446299

Following gene: 46446307

Centisome position: 33.8

GC content: 36.37

Gene sequence:

>1416_bases
ATGACAAATCCCTCCATTGTTTGGTTTCGACAAGATTTTCGTTTAGAAGATAATCCTGCTTTAAATGCTGCCACTCAGAA
AGGTGGGCCGGTCATTCCTGTATTTAATTGGGTTTTTAACCCAGAAAAAGAATGGCAATTAGGAGGTGCCAGTCAATGGT
GGCTTTATTATTCTTTAATAAGTTTAAAAAATGATTTAAGTGAATTAGGTCTCTCTTTAATTATTCGAAAAGAAGATCCT
TTAAAAAGTCTTTTAGAAATTGCTCACGAGACCGGAGCTGATACAGTTTATTGGAATCGTCGTTATGAGCCTATGTTAAT
ACAAGATGACGCTAAAATAAAAACAGAATTGCAAAAGCAGGGGATAAAAGCGCATAGTTTTAATGGGAATTTATTGTTTG
AACCTTGGACCATTGCGAATAAACAAGGAAAACCTTTTCAAGTATTCACTCCATTTTGGAATCAGTGTTTAAAATTAAAT
GATCCAGAAGTTCCTTTGCCCGTTCCACATTCGTTAAAAAAATTTGTAGGTCAACTTCAAACAGAGTCCATTGATTCTTT
AAATCTATTACCTAAAATTAAATGGGATAAAGGACTTAAAGAAGTTTGGTCTCCCGGTGCTAAAAGTGCTAAAGCTTTAT
TAAAAAAAGGATTAACAGGAGTTATAGATCAATACTTGGACATTCGCGATTTACCAGATCATGATGGAACATCCTTACTT
TCTCCTTATTTGCATTTTGGAGAGATTTCTCCGCGCATGATTTGGCAGGCTGTCAAAGAAAATTCAACTTCGAAAGGAGC
AGAGGGATATTTAAGACAAATTGGCTGGAGAGAATTTGCCCATCACTTACTTTATCATTTTCCAGAAACACCGCAAAAGC
CATTAAGATCGCAGTTTAATTCCTTTTCTTGGAAAAATGATAAGCAAAATTTGAAAGCTTGGCAAAAAGGTCAGACAGGG
TATCCTATTATTGATGCTGGGATGAGGCAACTTTGGAAAATAGGTTGGATGCATAATCGTGTCCGATTGATTGTCGGTTC
TTTTTTAGTTAAAGATTTGATGATTCATTGGATTGAAGGAGCTAAATGGTTTTGGGATACATTAGTTGACGCTGATTTAG
CGAATAATACAATGGGATGGCAATGGATTGCAGGTTGTGGTGCAGATGCAGCGCCTTATTTTCGTATTTTCAATCCTATT
ACGCAAGGTGAGAAATTTGATCCCGAAGGAAATTATGTCAAGAAATGGGTTCCAGAATTAATTAATCTTCCCAAGGAGTG
GCTACATCAACCTTGGGAAGCTCCTGAAGAAATATTGAGACAATCAGGGATTGAATTAGGAATAAACTATCCCAAACCTA
TAGTCAATCATGCGAAAGCTAGAGAAGAAGCTTTACAGGCTTACTCTCGCTTATAA

Upstream 100 bases:

>100_bases
TTGATTAAGATATTTTGACAAGTAATTTCTATATAAATTGTAGATTTTCTTTTTTTATCGGATATACCCAAAAGTAATAA
AAATGTTTTTTAGGTAGTTT

Downstream 100 bases:

>100_bases
TTAAGATCTTTTAATAGTCTTTTGAGAAAGATTTATTAAAAATCGGAAAATAAACTTAGCTTTGACTGGAAAAAGATTAA
AATGAAAATATTAGTCACAG

Product: putative photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTNPSIVWFRQDFRLEDNPALNAATQKGGPVIPVFNWVFNPEKEWQLGGASQWWLYYSLISLKNDLSELGLSLIIRKEDP
LKSLLEIAHETGADTVYWNRRYEPMLIQDDAKIKTELQKQGIKAHSFNGNLLFEPWTIANKQGKPFQVFTPFWNQCLKLN
DPEVPLPVPHSLKKFVGQLQTESIDSLNLLPKIKWDKGLKEVWSPGAKSAKALLKKGLTGVIDQYLDIRDLPDHDGTSLL
SPYLHFGEISPRMIWQAVKENSTSKGAEGYLRQIGWREFAHHLLYHFPETPQKPLRSQFNSFSWKNDKQNLKAWQKGQTG
YPIIDAGMRQLWKIGWMHNRVRLIVGSFLVKDLMIHWIEGAKWFWDTLVDADLANNTMGWQWIAGCGADAAPYFRIFNPI
TQGEKFDPEGNYVKKWVPELINLPKEWLHQPWEAPEEILRQSGIELGINYPKPIVNHAKAREEALQAYSRL

Sequences:

>Translated_471_residues
MTNPSIVWFRQDFRLEDNPALNAATQKGGPVIPVFNWVFNPEKEWQLGGASQWWLYYSLISLKNDLSELGLSLIIRKEDP
LKSLLEIAHETGADTVYWNRRYEPMLIQDDAKIKTELQKQGIKAHSFNGNLLFEPWTIANKQGKPFQVFTPFWNQCLKLN
DPEVPLPVPHSLKKFVGQLQTESIDSLNLLPKIKWDKGLKEVWSPGAKSAKALLKKGLTGVIDQYLDIRDLPDHDGTSLL
SPYLHFGEISPRMIWQAVKENSTSKGAEGYLRQIGWREFAHHLLYHFPETPQKPLRSQFNSFSWKNDKQNLKAWQKGQTG
YPIIDAGMRQLWKIGWMHNRVRLIVGSFLVKDLMIHWIEGAKWFWDTLVDADLANNTMGWQWIAGCGADAAPYFRIFNPI
TQGEKFDPEGNYVKKWVPELINLPKEWLHQPWEAPEEILRQSGIELGINYPKPIVNHAKAREEALQAYSRL
>Mature_470_residues
TNPSIVWFRQDFRLEDNPALNAATQKGGPVIPVFNWVFNPEKEWQLGGASQWWLYYSLISLKNDLSELGLSLIIRKEDPL
KSLLEIAHETGADTVYWNRRYEPMLIQDDAKIKTELQKQGIKAHSFNGNLLFEPWTIANKQGKPFQVFTPFWNQCLKLND
PEVPLPVPHSLKKFVGQLQTESIDSLNLLPKIKWDKGLKEVWSPGAKSAKALLKKGLTGVIDQYLDIRDLPDHDGTSLLS
PYLHFGEISPRMIWQAVKENSTSKGAEGYLRQIGWREFAHHLLYHFPETPQKPLRSQFNSFSWKNDKQNLKAWQKGQTGY
PIIDAGMRQLWKIGWMHNRVRLIVGSFLVKDLMIHWIEGAKWFWDTLVDADLANNTMGWQWIAGCGADAAPYFRIFNPIT
QGEKFDPEGNYVKKWVPELINLPKEWLHQPWEAPEEILRQSGIELGINYPKPIVNHAKAREEALQAYSRL

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=480, Percent_Identity=29.1666666666667, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4758072, Length=497, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI188536103, Length=435, Percent_Identity=28.5057471264368, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1786926, Length=475, Percent_Identity=36, Blast_Score=283, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324962, Length=514, Percent_Identity=30.3501945525292, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI17137248, Length=488, Percent_Identity=30.1229508196721, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24585455, Length=488, Percent_Identity=30.1229508196721, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24648152, Length=513, Percent_Identity=25.1461988304094, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54402; Mature: 54271

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPSIVWFRQDFRLEDNPALNAATQKGGPVIPVFNWVFNPEKEWQLGGASQWWLYYSLI
CCCCCEEEEEECCCCCCCCCCCCHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
SLKNDLSELGLSLIIRKEDPLKSLLEIAHETGADTVYWNRRYEPMLIQDDAKIKTELQKQ
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCEEECCCHHHHHHHHHC
GIKAHSFNGNLLFEPWTIANKQGKPFQVFTPFWNQCLKLNDPEVPLPVPHSLKKFVGQLQ
CCEEEECCCCEEECCCEECCCCCCCEEEECHHHHHHEECCCCCCCCCCCHHHHHHHHHHH
TESIDSLNLLPKIKWDKGLKEVWSPGAKSAKALLKKGLTGVIDQYLDIRDLPDHDGTSLL
HCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHH
SPYLHFGEISPRMIWQAVKENSTSKGAEGYLRQIGWREFAHHLLYHFPETPQKPLRSQFN
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SFSWKNDKQNLKAWQKGQTGYPIIDAGMRQLWKIGWMHNRVRLIVGSFLVKDLMIHWIEG
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AKWFWDTLVDADLANNTMGWQWIAGCGADAAPYFRIFNPITQGEKFDPEGNYVKKWVPEL
CHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHCHHCCCCCCCCCCCHHHHHHHHH
INLPKEWLHQPWEAPEEILRQSGIELGINYPKPIVNHAKAREEALQAYSRL
HCCCHHHHCCCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNPSIVWFRQDFRLEDNPALNAATQKGGPVIPVFNWVFNPEKEWQLGGASQWWLYYSLI
CCCCEEEEEECCCCCCCCCCCCHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
SLKNDLSELGLSLIIRKEDPLKSLLEIAHETGADTVYWNRRYEPMLIQDDAKIKTELQKQ
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCEEECCCHHHHHHHHHC
GIKAHSFNGNLLFEPWTIANKQGKPFQVFTPFWNQCLKLNDPEVPLPVPHSLKKFVGQLQ
CCEEEECCCCEEECCCEECCCCCCCEEEECHHHHHHEECCCCCCCCCCCHHHHHHHHHHH
TESIDSLNLLPKIKWDKGLKEVWSPGAKSAKALLKKGLTGVIDQYLDIRDLPDHDGTSLL
HCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHH
SPYLHFGEISPRMIWQAVKENSTSKGAEGYLRQIGWREFAHHLLYHFPETPQKPLRSQFN
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SFSWKNDKQNLKAWQKGQTGYPIIDAGMRQLWKIGWMHNRVRLIVGSFLVKDLMIHWIEG
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AKWFWDTLVDADLANNTMGWQWIAGCGADAAPYFRIFNPITQGEKFDPEGNYVKKWVPEL
CHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHCHHCCCCCCCCCCCHHHHHHHHH
INLPKEWLHQPWEAPEEILRQSGIELGINYPKPIVNHAKAREEALQAYSRL
HCCCHHHHCCCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]