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The map label for this gene is hemK [H]

Identifier: 46446287

GI number: 46446287

Start: 799626

End: 800465

Strand: Direct

Name: hemK [H]

Synonym: pc0653

Alternate gene names: 46446287

Gene position: 799626-800465 (Clockwise)

Preceding gene: 46446286

Following gene: 46446288

Centisome position: 33.12

GC content: 36.19

Gene sequence:

>840_bases
TTGGAGGGACTAACATTTTTAACGCACGAATTAGCAAAAAAAGATAAAAGCCTAGCTAAGCGAGAAGCTCAAGATTTACT
TGGCTCTATTCTCAAATGCGATCGTTCAACTCTTTATACTCGGCATTCTCAAGACTTGAGTTTAGAGGAATGGCAGACTT
GTCAAAATTATTTAAAAAGACGCATGAAGGGAGAGCCTTTAGCTTATATCCAAGGGAGCATTGATTTTTATGGCTGTTCT
ATCCAAGTTAACCCTTTCGTCTTAATTCCTCGGCAAGAAACAGAAATTTTAGTAGATAAAATTTCTACTTATCTATCTCA
TCAAAAAAAATTAAGTGGAAAAATTCTTTGGGACTTGTGTTCGGGATCTGGCTGTATAGGAATTGCTTTAAAAAAGAAAT
TTCCTCAATTACATGTCATTTCGAGTGATTTATCTTCTGCCGCATTATCATTAGCGCGTTCGAATGCACAGGATAATCAG
GTGGAAGTAGAGTTTTTACAAGGCGATTTACTCGAGCCGTTTGAAGGGCGTAGAGCGCATTTTATTATTTGTAATCCTCC
TTACATTTCTGAAGCAGAGTTTAAAGATTTAGATTTAGAGGTGAAAGAGTTTGAACCTAAAATGGCGCTTGTAGGTGGAG
AAACTGGTTTAGAAATTTACCAACGACTTGCAGAGATACTTCCTAATTATCTTTATCCTCATGCCAAAATTTGGTTTGAA
ATTGGCTATAAGCAAGGAGATTCTTTAAAGAAAATTTTCAAAAGCTCATATTGGAAAAGAGCCTTTCTTGAAAATGATTG
GGCCGGCCATCATCGCTTCTTTTTCCTTGAAAATGAATGA

Upstream 100 bases:

>100_bases
ATTATGTAATAAACGGAGATCTTGAAGAAATCACTTCAGCTCTTGTGGCTTATTTTCATCAGGAAAAGCTAAAAACAAGT
AGAGAAGAATGACCTCTATT

Downstream 100 bases:

>100_bases
TTTTTTGAGTATCCTACTTACCTTTGAGAATTAAGTCCTAAAGTGGCGACTGAATTAATGAGAATTTTTTGTTTTAAATT
CGTATGAGAATAAGTCTTGA

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; M.RprHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MEGLTFLTHELAKKDKSLAKREAQDLLGSILKCDRSTLYTRHSQDLSLEEWQTCQNYLKRRMKGEPLAYIQGSIDFYGCS
IQVNPFVLIPRQETEILVDKISTYLSHQKKLSGKILWDLCSGSGCIGIALKKKFPQLHVISSDLSSAALSLARSNAQDNQ
VEVEFLQGDLLEPFEGRRAHFIICNPPYISEAEFKDLDLEVKEFEPKMALVGGETGLEIYQRLAEILPNYLYPHAKIWFE
IGYKQGDSLKKIFKSSYWKRAFLENDWAGHHRFFFLENE

Sequences:

>Translated_279_residues
MEGLTFLTHELAKKDKSLAKREAQDLLGSILKCDRSTLYTRHSQDLSLEEWQTCQNYLKRRMKGEPLAYIQGSIDFYGCS
IQVNPFVLIPRQETEILVDKISTYLSHQKKLSGKILWDLCSGSGCIGIALKKKFPQLHVISSDLSSAALSLARSNAQDNQ
VEVEFLQGDLLEPFEGRRAHFIICNPPYISEAEFKDLDLEVKEFEPKMALVGGETGLEIYQRLAEILPNYLYPHAKIWFE
IGYKQGDSLKKIFKSSYWKRAFLENDWAGHHRFFFLENE
>Mature_279_residues
MEGLTFLTHELAKKDKSLAKREAQDLLGSILKCDRSTLYTRHSQDLSLEEWQTCQNYLKRRMKGEPLAYIQGSIDFYGCS
IQVNPFVLIPRQETEILVDKISTYLSHQKKLSGKILWDLCSGSGCIGIALKKKFPQLHVISSDLSSAALSLARSNAQDNQ
VEVEFLQGDLLEPFEGRRAHFIICNPPYISEAEFKDLDLEVKEFEPKMALVGGETGLEIYQRLAEILPNYLYPHAKIWFE
IGYKQGDSLKKIFKSSYWKRAFLENDWAGHHRFFFLENE

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=258, Percent_Identity=27.906976744186, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787463, Length=235, Percent_Identity=31.9148936170213, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87082085, Length=205, Percent_Identity=28.2926829268293, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324266, Length=231, Percent_Identity=32.9004329004329, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582226, Length=214, Percent_Identity=29.9065420560748, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32120; Mature: 32120

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLTFLTHELAKKDKSLAKREAQDLLGSILKCDRSTLYTRHSQDLSLEEWQTCQNYLKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCHHHHHHHHHHHHH
RMKGEPLAYIQGSIDFYGCSIQVNPFVLIPRQETEILVDKISTYLSHQKKLSGKILWDLC
HHCCCCEEEEECCCEEEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEC
SGSGCIGIALKKKFPQLHVISSDLSSAALSLARSNAQDNQVEVEFLQGDLLEPFEGRRAH
CCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEE
FIICNPPYISEAEFKDLDLEVKEFEPKMALVGGETGLEIYQRLAEILPNYLYPHAKIWFE
EEEECCCCCCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEE
IGYKQGDSLKKIFKSSYWKRAFLENDWAGHHRFFFLENE
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MEGLTFLTHELAKKDKSLAKREAQDLLGSILKCDRSTLYTRHSQDLSLEEWQTCQNYLKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCHHHHHHHHHHHHH
RMKGEPLAYIQGSIDFYGCSIQVNPFVLIPRQETEILVDKISTYLSHQKKLSGKILWDLC
HHCCCCEEEEECCCEEEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEEC
SGSGCIGIALKKKFPQLHVISSDLSSAALSLARSNAQDNQVEVEFLQGDLLEPFEGRRAH
CCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEE
FIICNPPYISEAEFKDLDLEVKEFEPKMALVGGETGLEIYQRLAEILPNYLYPHAKIWFE
EEEECCCCCCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEE
IGYKQGDSLKKIFKSSYWKRAFLENDWAGHHRFFFLENE
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]