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The map label for this gene is prfA

Identifier: 46446286

GI number: 46446286

Start: 798538

End: 799617

Strand: Direct

Name: prfA

Synonym: pc0652

Alternate gene names: 46446286

Gene position: 798538-799617 (Clockwise)

Preceding gene: 46446285

Following gene: 46446287

Centisome position: 33.07

GC content: 37.69

Gene sequence:

>1080_bases
TTGATGGAAAAAAAAGTTCATGAACTTTTGGAACGTTTAGCAGAAGTTGAAGAAGTTCTTGGAAACCCTCATGTTTATGA
TGATCAAAAAAAATATCGAGCTTTAACGCAAGAACATGCCTATTTAAATAATTTGAAAAAATCTTGGGATGAGAAGAAAG
ATTTAGAAAAGCAATTAGAAGATAATCAAGAATTGCTTAAAATTGAAAAAGATTTAGAATTTGCGGAAGTGTTAAAAGAT
GATATTCGTCAACTTCAATCTCGTGTGCAAGAGTTACAACTTCTTATTGAAAATTTACTTGTTCCACCCGATCCTTTAGA
TAACCGTCCCTCAATTATTGAGCTCAGAGCAGGAACAGGCGGAGATGAAGCTGCTTTATTTGTGGGTGACTGTGTAAGAA
TGTACAAGCTCTTTGCAGATCGTAAAGGGTGGCGTTATGAGTTACTCTCTTGCACTCCTTCTGAAATGGGAGGATTTAAA
GAGTATGTAATGGTTTTGTCTGGCGAGAATGTTTATCGTTTTATGCAATATGAAGCTGGAACTCATCGTGTACAAAGAGT
TCCTGCAACTGAAGCTCAAGGACGTGTGCATACTTCAGCCATTACGATTGCCGTTTTACCTGAACCGGATGAAAATGAAG
AAATTGATTTGGATGAGAAAGATTTAAGAATTGATACTTACCGATCTTCGGGTGCAGGAGGTCAGCATGTCAATACAACA
GACAGCGCTGTCCGCATTACCCACGTTCCATCTGGTATTGTTGTTTATTGTCAAGAAGAACGTAGCCAGCATAAGAATAA
AGACAAGGCGATGCGCCTTTTGAAGGCTAAAATGGCCGAAATCGAGCAACAAAAAAAACAACAAGAACTCGCCAGTACTA
GAGCCCAACAAGTGGGATCGGGTGATCGCTCCGAACGAATTCGTACCTATAATTTTCCTCAAAATCGAATTACAGATCAT
CGAATCAATTTAACAAAATACAATTTAGATTATGTAATAAACGGAGATCTTGAAGAAATCACTTCAGCTCTTGTGGCTTA
TTTTCATCAGGAAAAGCTAAAAACAAGTAGAGAAGAATGA

Upstream 100 bases:

>100_bases
ATTTTGATGTTTCCGTTTCAGTTTAAATCTAAGAGAGAGGTTTTTTGATCAGATAATTTCTAAGAAAACATTCTCAGCTT
CATAAGGAATAAAAAGTATT

Downstream 100 bases:

>100_bases
CCTCTATTTTGGAGGGACTAACATTTTTAACGCACGAATTAGCAAAAAAAGATAAAAGCCTAGCTAAGCGAGAAGCTCAA
GATTTACTTGGCTCTATTCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MMEKKVHELLERLAEVEEVLGNPHVYDDQKKYRALTQEHAYLNNLKKSWDEKKDLEKQLEDNQELLKIEKDLEFAEVLKD
DIRQLQSRVQELQLLIENLLVPPDPLDNRPSIIELRAGTGGDEAALFVGDCVRMYKLFADRKGWRYELLSCTPSEMGGFK
EYVMVLSGENVYRFMQYEAGTHRVQRVPATEAQGRVHTSAITIAVLPEPDENEEIDLDEKDLRIDTYRSSGAGGQHVNTT
DSAVRITHVPSGIVVYCQEERSQHKNKDKAMRLLKAKMAEIEQQKKQQELASTRAQQVGSGDRSERIRTYNFPQNRITDH
RINLTKYNLDYVINGDLEEITSALVAYFHQEKLKTSREE

Sequences:

>Translated_359_residues
MMEKKVHELLERLAEVEEVLGNPHVYDDQKKYRALTQEHAYLNNLKKSWDEKKDLEKQLEDNQELLKIEKDLEFAEVLKD
DIRQLQSRVQELQLLIENLLVPPDPLDNRPSIIELRAGTGGDEAALFVGDCVRMYKLFADRKGWRYELLSCTPSEMGGFK
EYVMVLSGENVYRFMQYEAGTHRVQRVPATEAQGRVHTSAITIAVLPEPDENEEIDLDEKDLRIDTYRSSGAGGQHVNTT
DSAVRITHVPSGIVVYCQEERSQHKNKDKAMRLLKAKMAEIEQQKKQQELASTRAQQVGSGDRSERIRTYNFPQNRITDH
RINLTKYNLDYVINGDLEEITSALVAYFHQEKLKTSREE
>Mature_359_residues
MMEKKVHELLERLAEVEEVLGNPHVYDDQKKYRALTQEHAYLNNLKKSWDEKKDLEKQLEDNQELLKIEKDLEFAEVLKD
DIRQLQSRVQELQLLIENLLVPPDPLDNRPSIIELRAGTGGDEAALFVGDCVRMYKLFADRKGWRYELLSCTPSEMGGFK
EYVMVLSGENVYRFMQYEAGTHRVQRVPATEAQGRVHTSAITIAVLPEPDENEEIDLDEKDLRIDTYRSSGAGGQHVNTT
DSAVRITHVPSGIVVYCQEERSQHKNKDKAMRLLKAKMAEIEQQKKQQELASTRAQQVGSGDRSERIRTYNFPQNRITDH
RINLTKYNLDYVINGDLEEITSALVAYFHQEKLKTSREE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=300, Percent_Identity=46.6666666666667, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI34577120, Length=309, Percent_Identity=41.4239482200647, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=46.4285714285714, Blast_Score=178, Evalue=6e-45,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=49.0140845070423, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI2367172, Length=333, Percent_Identity=36.3363363363363, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=326, Percent_Identity=35.8895705521472, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=41.7417417417417, Blast_Score=238, Evalue=8e-64,
Organism=Drosophila melanogaster, GI19921226, Length=285, Percent_Identity=42.4561403508772, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PARUW (Q6MDH3)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007651.1
- ProteinModelPortal:   Q6MDH3
- SMR:   Q6MDH3
- STRING:   Q6MDH3
- GeneID:   2779420
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0652
- NMPDR:   fig|264201.1.peg.652
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- PhylomeDB:   Q6MDH3
- ProtClustDB:   PRK00591
- BioCyc:   CPRO264201:PC0652-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41478; Mature: 41478

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEKKVHELLERLAEVEEVLGNPHVYDDQKKYRALTQEHAYLNNLKKSWDEKKDLEKQLE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DNQELLKIEKDLEFAEVLKDDIRQLQSRVQELQLLIENLLVPPDPLDNRPSIIELRAGTG
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
GDEAALFVGDCVRMYKLFADRKGWRYELLSCTPSEMGGFKEYVMVLSGENVYRFMQYEAG
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHHHHHEEECCCCHHHHHHHCCC
THRVQRVPATEAQGRVHTSAITIAVLPEPDENEEIDLDEKDLRIDTYRSSGAGGQHVNTT
HHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
DSAVRITHVPSGIVVYCQEERSQHKNKDKAMRLLKAKMAEIEQQKKQQELASTRAQQVGS
CCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQNRITDHRINLTKYNLDYVINGDLEEITSALVAYFHQEKLKTSREE
CCCCCCCEECCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMEKKVHELLERLAEVEEVLGNPHVYDDQKKYRALTQEHAYLNNLKKSWDEKKDLEKQLE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DNQELLKIEKDLEFAEVLKDDIRQLQSRVQELQLLIENLLVPPDPLDNRPSIIELRAGTG
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
GDEAALFVGDCVRMYKLFADRKGWRYELLSCTPSEMGGFKEYVMVLSGENVYRFMQYEAG
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHHHHHEEECCCCHHHHHHHCCC
THRVQRVPATEAQGRVHTSAITIAVLPEPDENEEIDLDEKDLRIDTYRSSGAGGQHVNTT
HHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
DSAVRITHVPSGIVVYCQEERSQHKNKDKAMRLLKAKMAEIEQQKKQQELASTRAQQVGS
CCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQNRITDHRINLTKYNLDYVINGDLEEITSALVAYFHQEKLKTSREE
CCCCCCCEECCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA