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The map label for this gene is dagA [C]

Identifier: 46446256

GI number: 46446256

Start: 763580

End: 764959

Strand: Direct

Name: dagA [C]

Synonym: pc0622

Alternate gene names: 46446256

Gene position: 763580-764959 (Clockwise)

Preceding gene: 46446248

Following gene: 46446259

Centisome position: 31.63

GC content: 35.87

Gene sequence:

>1380_bases
ATGGATTTTAATCTAAAAATTTTTTTAATAAATTTTAATGAAGGATTTACGTTTTTTCTGATTTTTCCGAGTATCATCCT
TTTTGGACTTTATCTAACGTTTAAATTGCAATTTGTTCAAATATCAAAGCTAAAAATGAGTTTTTGTTGCTTACTTAAAA
AAGATCAAGGATGTCAAGGAAATATCAGCCATTATCAAGCAATTTCAGCCGTTTTAGCCGGTAATTTTGGGACGGGAAAT
ATTTCGGGTATGGCTGTTGCTATTTCAACAGGTGGTCCAGGTGCCCTTGTTTGGATGTGGATTATGGCTTTTTTAGGTGC
CTCTATTCAGTATGTTAGTTGTGTTTTAGGTGTTAAATATCGAAAACTTAACTCTAATGGTGAGTATGTAGGGGGGCCTA
TGTACTATTTATCCGAGGGTCTTAATATGAAAGCATTAGGAACGTTGTTTAGCCTATTTACCATTTTTGGTGCTTTAACA
GTTGGAAATTTTGCACAAATCAATTCAGCAACACTTCCATTGATAAAATTAGGTTTAGATCCTTTTATTTGTAGCTTATT
TATTGCTTTATTTGTTGGTGTTGTCTTAGTAGGAGGAGTGCAAAGAGTTGCCAAGCTTGCTTCAATTATTGTTCCTGTTA
AGGCAGCCCTTTATTTAGGAACAGCATTTGTTATTCTTTGGCTTCATGCCGATCAAGTGATGCCTGCTTTCGAACTCATG
ATAAAAGCTGCGTTTGTTCCGCAATCTGCTTTTGGAGGAGTGCTTGGATTTAGTGTTGTTAAAGCTATCTCTACAGGATT
TGATCGGGGAGTTTTTGCAACTGATGCCGGAACAGGGATTGTGCCAATTTTGCAATCGAGTGCTCGTACGACTAATCCTA
TTGTAGATGGAGTGGTTACTTTAGTTGCTTCTTTTTTAGTAATGATTGTTTGTACAGCGACAGGGCTAACTTTGATTGTG
ACAGGAGCTTGGCAAGTGGAGGGATTACAGAGCACCAATATGGTTACCCACGCTTTTCAAGTAGGCTTAGGAAGCTCACT
TGGAGCATTTATCGTAATTACTGCCCTGATTTTATTTGCTTATACGACTATTCTAGCTTGGGCATATTGTGCAGAAAAAG
CTGTAGATTTTTTGTGGGGAAGTAAATATGCAGGCTATTTTAAGTATATTTATATTGTTTTTATTCCCATCGGAGCCCTA
ATTCATGTTGATTTGATTTGGAGGCTTGCCGATATCGCAATTTCTTTAATGTTGTTTACTAATTTGATAGGTATTATCAG
CTTGTCTAAAGAAGTTATCGATGATAATCGTTTATTTTTTTTAAAAAAGTCGGATTATTTATCGCATACAAATTTAATGC
CCACAACGCCAAATGAATAA

Upstream 100 bases:

>100_bases
TTTTTAACAATTAATGAATAGTTAATTTTTTTAATTTAAAAGCATATTACATATTAATATCAAAAAAAATATTTACAAAA
TTAATTAGTAACAGGTAAAA

Downstream 100 bases:

>100_bases
AAATTATTTTAAATATAAAAGGTAAGAAAAAATATTAATTACCTGCTTGACTTTGTTTTCTAAAAGCATTAGCAGCTAAG
TGAGCTTGCCTAGTTGGTTC

Product: putative D-alanine/glycine transport protein, sodium-dependent

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDFNLKIFLINFNEGFTFFLIFPSIILFGLYLTFKLQFVQISKLKMSFCCLLKKDQGCQGNISHYQAISAVLAGNFGTGN
ISGMAVAISTGGPGALVWMWIMAFLGASIQYVSCVLGVKYRKLNSNGEYVGGPMYYLSEGLNMKALGTLFSLFTIFGALT
VGNFAQINSATLPLIKLGLDPFICSLFIALFVGVVLVGGVQRVAKLASIIVPVKAALYLGTAFVILWLHADQVMPAFELM
IKAAFVPQSAFGGVLGFSVVKAISTGFDRGVFATDAGTGIVPILQSSARTTNPIVDGVVTLVASFLVMIVCTATGLTLIV
TGAWQVEGLQSTNMVTHAFQVGLGSSLGAFIVITALILFAYTTILAWAYCAEKAVDFLWGSKYAGYFKYIYIVFIPIGAL
IHVDLIWRLADIAISLMLFTNLIGIISLSKEVIDDNRLFFLKKSDYLSHTNLMPTTPNE

Sequences:

>Translated_459_residues
MDFNLKIFLINFNEGFTFFLIFPSIILFGLYLTFKLQFVQISKLKMSFCCLLKKDQGCQGNISHYQAISAVLAGNFGTGN
ISGMAVAISTGGPGALVWMWIMAFLGASIQYVSCVLGVKYRKLNSNGEYVGGPMYYLSEGLNMKALGTLFSLFTIFGALT
VGNFAQINSATLPLIKLGLDPFICSLFIALFVGVVLVGGVQRVAKLASIIVPVKAALYLGTAFVILWLHADQVMPAFELM
IKAAFVPQSAFGGVLGFSVVKAISTGFDRGVFATDAGTGIVPILQSSARTTNPIVDGVVTLVASFLVMIVCTATGLTLIV
TGAWQVEGLQSTNMVTHAFQVGLGSSLGAFIVITALILFAYTTILAWAYCAEKAVDFLWGSKYAGYFKYIYIVFIPIGAL
IHVDLIWRLADIAISLMLFTNLIGIISLSKEVIDDNRLFFLKKSDYLSHTNLMPTTPNE
>Mature_459_residues
MDFNLKIFLINFNEGFTFFLIFPSIILFGLYLTFKLQFVQISKLKMSFCCLLKKDQGCQGNISHYQAISAVLAGNFGTGN
ISGMAVAISTGGPGALVWMWIMAFLGASIQYVSCVLGVKYRKLNSNGEYVGGPMYYLSEGLNMKALGTLFSLFTIFGALT
VGNFAQINSATLPLIKLGLDPFICSLFIALFVGVVLVGGVQRVAKLASIIVPVKAALYLGTAFVILWLHADQVMPAFELM
IKAAFVPQSAFGGVLGFSVVKAISTGFDRGVFATDAGTGIVPILQSSARTTNPIVDGVVTLVASFLVMIVCTATGLTLIV
TGAWQVEGLQSTNMVTHAFQVGLGSSLGAFIVITALILFAYTTILAWAYCAEKAVDFLWGSKYAGYFKYIYIVFIPIGAL
IHVDLIWRLADIAISLMLFTNLIGIISLSKEVIDDNRLFFLKKSDYLSHTNLMPTTPNE

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=434, Percent_Identity=29.4930875576037, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49631; Mature: 49631

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNLKIFLINFNEGFTFFLIFPSIILFGLYLTFKLQFVQISKLKMSFCCLLKKDQGCQG
CCCEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
NISHYQAISAVLAGNFGTGNISGMAVAISTGGPGALVWMWIMAFLGASIQYVSCVLGVKY
CHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHE
RKLNSNGEYVGGPMYYLSEGLNMKALGTLFSLFTIFGALTVGNFAQINSATLPLIKLGLD
EEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHCCC
PFICSLFIALFVGVVLVGGVQRVAKLASIIVPVKAALYLGTAFVILWLHADQVMPAFELM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IKAAFVPQSAFGGVLGFSVVKAISTGFDRGVFATDAGTGIVPILQSSARTTNPIVDGVVT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCCCCCHHHHHHHH
LVASFLVMIVCTATGLTLIVTGAWQVEGLQSTNMVTHAFQVGLGSSLGAFIVITALILFA
HHHHHHHHHHHHHCCCEEEEECCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YTTILAWAYCAEKAVDFLWGSKYAGYFKYIYIVFIPIGALIHVDLIWRLADIAISLMLFT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIGIISLSKEVIDDNRLFFLKKSDYLSHTNLMPTTPNE
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDFNLKIFLINFNEGFTFFLIFPSIILFGLYLTFKLQFVQISKLKMSFCCLLKKDQGCQG
CCCEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
NISHYQAISAVLAGNFGTGNISGMAVAISTGGPGALVWMWIMAFLGASIQYVSCVLGVKY
CHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHE
RKLNSNGEYVGGPMYYLSEGLNMKALGTLFSLFTIFGALTVGNFAQINSATLPLIKLGLD
EEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHCCC
PFICSLFIALFVGVVLVGGVQRVAKLASIIVPVKAALYLGTAFVILWLHADQVMPAFELM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IKAAFVPQSAFGGVLGFSVVKAISTGFDRGVFATDAGTGIVPILQSSARTTNPIVDGVVT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCCCCCHHHHHHHH
LVASFLVMIVCTATGLTLIVTGAWQVEGLQSTNMVTHAFQVGLGSSLGAFIVITALILFA
HHHHHHHHHHHHHCCCEEEEECCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YTTILAWAYCAEKAVDFLWGSKYAGYFKYIYIVFIPIGALIHVDLIWRLADIAISLMLFT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIGIISLSKEVIDDNRLFFLKKSDYLSHTNLMPTTPNE
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]