Click here to switch to the map view.

The map label for this gene is dnaK [H]

Identifier: 46446225

GI number: 46446225

Start: 727667

End: 729502

Strand: Direct

Name: dnaK [H]

Synonym: pc0591

Alternate gene names: 46446225

Gene position: 727667-729502 (Clockwise)

Preceding gene: 46446224

Following gene: 46446228

Centisome position: 30.14

GC content: 37.15

Gene sequence:

>1836_bases
ATGAAAGAAGAAGCAACATATATTATTGGAATTGATTTCGGAACAACGAATTGTACATTAGCTTACACACCCCTAAAAAA
TAATAACACGTTGCCTGTCATTCAACAAAGTGAGTTAACACAAATTGTTTCTGAACAGACAATAGGAAATTCTTTCTCAC
TTCCTTCTTTCATTTACTACCCATTAGCCGAAGAGCTTTCACAAAGCGTGGCAGCTATAAATTGGGATTTAGCTTCTTCC
TTTACAATTGGCATATTTGCTAAAGAAAGGGGAGCAGAACTACCTACTAGATTGGTATCATCAGCAAAATCTTGGTTATG
TCACAATGCAATTGATCGGAGACAAGCAGTTTTACCTTTTGAATCGGAAGAATTGATAAATAAAATAAGCCCTTTAGATG
CTTGTGCAAAAATCTTCCAACATCTTCGTCAATCTTGGGATTTCAATCAACCCAATGCTCCTTTTATCAGACAGAACATT
TTTATTACTGTTCCGGCTTCTTTTGATCCGAGTGCTAGACAATTCGTTCAAGAGGCAGCTGAGCTAGCGGGTTATCCAGA
AATCATTTTATTAGAAGAGCCGCAAGCTGCTTTTTATGCCTGGCTATATGCTCATGCTAATGATTGGAGAAACTTATTTA
AAGTTAATGAGACAATTTTAGTCATTGATATTGGAGGAGGGACAACAGACTTTAGCTTAATTAAAGTAGAAGAAGAGAAT
GGGAATTTAACTTTAAGAAGATTGGCTGTAGGATCTCATTTACTTTTGGGGGGAGATAATATCGATCTTAGTTTGGCTTA
TCTTGCCAAACAAAAGCTAGAAGAAGAAGGCTATACTATTGATCATTGGCAGCTTCAAAGTCTTATTTACCAAGCTAGAG
CTGCTAAAGAATTATTAATGAATGAAAATCCTCCTGAAAAAGTCGAAATGACAATCCTTGGGAGAGGAAGTAAGTTAATA
GGGAATACTCTTACAACACAATTATCTCAGCAAGAAGTTGAAAAATTCATTTTGGAAGGATTTATTCCTCTCGTTAAACC
GGATGAAAAATCTCAACAAGAGCGTCATTTAGGAATTCAGCAGATTGGTCTACCTTATGTTCAAGATCCACGGATATCTT
GCCAATTGGCAAAATTTTTATCGATGACAGGGGAGTCAGAGTCGGCCAACATGGATCAATTTATTCTTCCCTCAAAAATA
TTGTTTAATGGTGGAACTTTGAAGGCAAAAAAGTTGCGAGATCAGCTTTTAGAATTGCTTAATCAATGGGCTAAAAAACT
TAATAAACCCCCAGTCACTGAAATTCTCGGTGCAGACTATGATTATGCAGTTAGCAAAGGGGCAGTTTATTATGGTTTAG
CTCGGCTTGGGCATACGATTCGTATTCGTGGTGGAACAAGTCGTAGTTATTTCATTGGAGTTGAGGAAGCAGTTCCGGCT
GTCCCAGGAATTAGCCCTCCTTTAAGAGCTATCTGTATCGTTCCGTTTGGAATGGAAGAAGGGGAGGAACGAGAGTTAGA
TCAACAAGAATTTTCTCTGGTTTTAGGAGAAATGGCAACATTTCGATTTTTTAGTCATATAACACCCCAACTATCCAACG
GGACTGAACCAGTAGCTGGAACTATTGTTCGAAATTGGAAACAAGAACTTACTGAGCTTCATCCCATTGAAACAGTTTTA
AATAAAACAGAAGAAGATGGAAAAATGATTCGTGTGAGATTGAAATCTAAAATCACTGAACTTGGTGTTCTTGAGCTTTG
GTGTGTTGCTTCAGATGGAAGAAAATGGAAACTTGAATTTGATATTCGAGACCATAAAAATCAAAACTCAGCTTAA

Upstream 100 bases:

>100_bases
GCGCTTAAACGTTCTTTACCAAAAAAAACTGGTGAAACGATAACAGATATCATCTGCCCAGCAGAAATAGAAGTAAAATG
ATTTGTTTTAAGGAATAAGC

Downstream 100 bases:

>100_bases
ATTGGCAACTAAGAAATGCAAAAATATAAGTGAAACAAACAGGTTGTTTCACTTGTTGCCCCTAATTAATTTCTCAAATG
AGTAAAGTAGATAGAAGTAT

Product: putative heat shock protein 70 (chaperone protein dnaK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MKEEATYIIGIDFGTTNCTLAYTPLKNNNTLPVIQQSELTQIVSEQTIGNSFSLPSFIYYPLAEELSQSVAAINWDLASS
FTIGIFAKERGAELPTRLVSSAKSWLCHNAIDRRQAVLPFESEELINKISPLDACAKIFQHLRQSWDFNQPNAPFIRQNI
FITVPASFDPSARQFVQEAAELAGYPEIILLEEPQAAFYAWLYAHANDWRNLFKVNETILVIDIGGGTTDFSLIKVEEEN
GNLTLRRLAVGSHLLLGGDNIDLSLAYLAKQKLEEEGYTIDHWQLQSLIYQARAAKELLMNENPPEKVEMTILGRGSKLI
GNTLTTQLSQQEVEKFILEGFIPLVKPDEKSQQERHLGIQQIGLPYVQDPRISCQLAKFLSMTGESESANMDQFILPSKI
LFNGGTLKAKKLRDQLLELLNQWAKKLNKPPVTEILGADYDYAVSKGAVYYGLARLGHTIRIRGGTSRSYFIGVEEAVPA
VPGISPPLRAICIVPFGMEEGEERELDQQEFSLVLGEMATFRFFSHITPQLSNGTEPVAGTIVRNWKQELTELHPIETVL
NKTEEDGKMIRVRLKSKITELGVLELWCVASDGRKWKLEFDIRDHKNQNSA

Sequences:

>Translated_611_residues
MKEEATYIIGIDFGTTNCTLAYTPLKNNNTLPVIQQSELTQIVSEQTIGNSFSLPSFIYYPLAEELSQSVAAINWDLASS
FTIGIFAKERGAELPTRLVSSAKSWLCHNAIDRRQAVLPFESEELINKISPLDACAKIFQHLRQSWDFNQPNAPFIRQNI
FITVPASFDPSARQFVQEAAELAGYPEIILLEEPQAAFYAWLYAHANDWRNLFKVNETILVIDIGGGTTDFSLIKVEEEN
GNLTLRRLAVGSHLLLGGDNIDLSLAYLAKQKLEEEGYTIDHWQLQSLIYQARAAKELLMNENPPEKVEMTILGRGSKLI
GNTLTTQLSQQEVEKFILEGFIPLVKPDEKSQQERHLGIQQIGLPYVQDPRISCQLAKFLSMTGESESANMDQFILPSKI
LFNGGTLKAKKLRDQLLELLNQWAKKLNKPPVTEILGADYDYAVSKGAVYYGLARLGHTIRIRGGTSRSYFIGVEEAVPA
VPGISPPLRAICIVPFGMEEGEERELDQQEFSLVLGEMATFRFFSHITPQLSNGTEPVAGTIVRNWKQELTELHPIETVL
NKTEEDGKMIRVRLKSKITELGVLELWCVASDGRKWKLEFDIRDHKNQNSA
>Mature_611_residues
MKEEATYIIGIDFGTTNCTLAYTPLKNNNTLPVIQQSELTQIVSEQTIGNSFSLPSFIYYPLAEELSQSVAAINWDLASS
FTIGIFAKERGAELPTRLVSSAKSWLCHNAIDRRQAVLPFESEELINKISPLDACAKIFQHLRQSWDFNQPNAPFIRQNI
FITVPASFDPSARQFVQEAAELAGYPEIILLEEPQAAFYAWLYAHANDWRNLFKVNETILVIDIGGGTTDFSLIKVEEEN
GNLTLRRLAVGSHLLLGGDNIDLSLAYLAKQKLEEEGYTIDHWQLQSLIYQARAAKELLMNENPPEKVEMTILGRGSKLI
GNTLTTQLSQQEVEKFILEGFIPLVKPDEKSQQERHLGIQQIGLPYVQDPRISCQLAKFLSMTGESESANMDQFILPSKI
LFNGGTLKAKKLRDQLLELLNQWAKKLNKPPVTEILGADYDYAVSKGAVYYGLARLGHTIRIRGGTSRSYFIGVEEAVPA
VPGISPPLRAICIVPFGMEEGEERELDQQEFSLVLGEMATFRFFSHITPQLSNGTEPVAGTIVRNWKQELTELHPIETVL
NKTEEDGKMIRVRLKSKITELGVLELWCVASDGRKWKLEFDIRDHKNQNSA

Specific function: Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by hscB [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=308, Percent_Identity=25.3246753246753, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788875, Length=272, Percent_Identity=26.8382352941176, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126
- InterPro:   IPR010236 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 68647; Mature: 68647

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEATYIIGIDFGTTNCTLAYTPLKNNNTLPVIQQSELTQIVSEQTIGNSFSLPSFIYY
CCCCCEEEEEEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHH
PLAEELSQSVAAINWDLASSFTIGIFAKERGAELPTRLVSSAKSWLCHNAIDRRQAVLPF
HHHHHHHHHHHEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
ESEELINKISPLDACAKIFQHLRQSWDFNQPNAPFIRQNIFITVPASFDPSARQFVQEAA
CHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHH
ELAGYPEIILLEEPQAAFYAWLYAHANDWRNLFKVNETILVIDIGGGTTDFSLIKVEEEN
HHCCCCEEEEEECCCHHEEHHHHHHHHHHHHHHEECCEEEEEECCCCCCCEEEEEEEECC
GNLTLRRLAVGSHLLLGGDNIDLSLAYLAKQKLEEEGYTIDHWQLQSLIYQARAAKELLM
CCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHH
NENPPEKVEMTILGRGSKLIGNTLTTQLSQQEVEKFILEGFIPLVKPDEKSQQERHLGIQ
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHH
QIGLPYVQDPRISCQLAKFLSMTGESESANMDQFILPSKILFNGGTLKAKKLRDQLLELL
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEECCCCEEHHHHHHHHHHHH
NQWAKKLNKPPVTEILGADYDYAVSKGAVYYGLARLGHTIRIRGGTSRSYFIGVEEAVPA
HHHHHHCCCCCHHHHHCCCCCHHHCCCHHHHHHHHCCCEEEEECCCCCEEEEEHHHHCCC
VPGISPPLRAICIVPFGMEEGEERELDQQEFSLVLGEMATFRFFSHITPQLSNGTEPVAG
CCCCCCCCEEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
TIVRNWKQELTELHPIETVLNKTEEDGKMIRVRLKSKITELGVLELWCVASDGRKWKLEF
HHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEEEEHHHHHHCCCEEEEEEECCCCEEEEEE
DIRDHKNQNSA
EECCCCCCCCC
>Mature Secondary Structure
MKEEATYIIGIDFGTTNCTLAYTPLKNNNTLPVIQQSELTQIVSEQTIGNSFSLPSFIYY
CCCCCEEEEEEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHH
PLAEELSQSVAAINWDLASSFTIGIFAKERGAELPTRLVSSAKSWLCHNAIDRRQAVLPF
HHHHHHHHHHHEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
ESEELINKISPLDACAKIFQHLRQSWDFNQPNAPFIRQNIFITVPASFDPSARQFVQEAA
CHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHH
ELAGYPEIILLEEPQAAFYAWLYAHANDWRNLFKVNETILVIDIGGGTTDFSLIKVEEEN
HHCCCCEEEEEECCCHHEEHHHHHHHHHHHHHHEECCEEEEEECCCCCCCEEEEEEEECC
GNLTLRRLAVGSHLLLGGDNIDLSLAYLAKQKLEEEGYTIDHWQLQSLIYQARAAKELLM
CCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHH
NENPPEKVEMTILGRGSKLIGNTLTTQLSQQEVEKFILEGFIPLVKPDEKSQQERHLGIQ
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHH
QIGLPYVQDPRISCQLAKFLSMTGESESANMDQFILPSKILFNGGTLKAKKLRDQLLELL
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEECCCCEEHHHHHHHHHHHH
NQWAKKLNKPPVTEILGADYDYAVSKGAVYYGLARLGHTIRIRGGTSRSYFIGVEEAVPA
HHHHHHCCCCCHHHHHCCCCCHHHCCCHHHHHHHHCCCEEEEECCCCCEEEEEHHHHCCC
VPGISPPLRAICIVPFGMEEGEERELDQQEFSLVLGEMATFRFFSHITPQLSNGTEPVAG
CCCCCCCCEEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
TIVRNWKQELTELHPIETVLNKTEEDGKMIRVRLKSKITELGVLELWCVASDGRKWKLEF
HHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEEEEHHHHHHCCCEEEEEEECCCCEEEEEE
DIRDHKNQNSA
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA