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The map label for this gene is cysD

Identifier: 45659190

GI number: 45659190

Start: 4133958

End: 4134875

Strand: Reverse

Name: cysD

Synonym: LIC13371

Alternate gene names: 45659190

Gene position: 4134875-4133958 (Counterclockwise)

Preceding gene: 45659191

Following gene: 45659189

Centisome position: 96.67

GC content: 40.63

Gene sequence:

>918_bases
ATGACTTCAACCATGAATAGATCTAGACTTACACATCTTGAACAACTAGAAGCAGAATCGATCTATATTCTTAGAGAAAC
CGCTTCTCAGTTTGAAAGACCTGCTCTGCTTTTTTCCGGCGGAAAAGATTCGATCACGCTCGTACATCTCGCATTGAAAG
CGTTTCGTCCCGGAAAGTTTCCGTTTCCACTTGTTCATATTGATACCGGCCATAACTTTCAAGAAGCTCTCGATTTTAGA
GATGAGCTTGCTTCCAAAATCGGTGAAAAACTGATTATTCGTTATGTTCAAGATTCTATCGATCAGGGAAAAGCTGTTGA
AGAAAAAGGAAAATTTCCAAGCAGAAATGGAATTCAAACCGTGACCCTTTTGGATACAATTGCGGAATTTAAATTTGACG
CTTGTATCGGAGGTGCTCGTAGAGACGAAGAAAAAGCAAGAGCAAAAGAAAGAGTTTTTTCGGTTCGTGATGAGTTTGGT
CAGTGGGATCCAAAATTACAAAGACCCGAACTTTGGAATATTTATAACGGTAGAATTAGTCCAGGGGAAAATGTTCGTGT
TTTTCCAATTTCCAATTGGACCGAACTGGACGTTTGGGAATATATCCGTAAAGAAAACATTGCACTTCCTTCCTTATACT
TTTCTCATAAACGACAAGTGATTTACAGAGAAAATCTTCTCTTCCCGGTTTCTAAGTTCATTACGATCGATGCAAACGAT
CGGGTGGAAGATAAAATTGTTCGTTTTCGCACCGTAGGAGACATGACTTGTACTGCCGCCGTGGATTCTCAAGCGGATAA
CATAGACGATATTATTCTCGAAATCCAAACGACTCGGACCACAGAGAGAGGTTCCAGACTAGACGATAAACGCTCCGAAG
CCGCTATGGAAGATAGAAAAAGAGGAGGTTACTTCTGA

Upstream 100 bases:

>100_bases
GCGCCGGAAGATGGTGGTGGGAAGAATCCAACCAAGAATGTGGACTTCACGTTGTAGACGGCAAGTTAGTTCGTCAAAAG
CTTGGTCCTAAAAGAGCAAT

Downstream 100 bases:

>100_bases
TGGATTTATTACGTTTTATCACTGCAGGAAGTGTGGACGACGGTAAATCCACTTTGATCGGAAGACTTCTCTACGACAGC
AAATCGATCTTCGAAGATCA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKFPFPLVHIDTGHNFQEALDFR
DELASKIGEKLIIRYVQDSIDQGKAVEEKGKFPSRNGIQTVTLLDTIAEFKFDACIGGARRDEEKARAKERVFSVRDEFG
QWDPKLQRPELWNIYNGRISPGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDAND
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRKRGGYF

Sequences:

>Translated_305_residues
MTSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKFPFPLVHIDTGHNFQEALDFR
DELASKIGEKLIIRYVQDSIDQGKAVEEKGKFPSRNGIQTVTLLDTIAEFKFDACIGGARRDEEKARAKERVFSVRDEFG
QWDPKLQRPELWNIYNGRISPGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDAND
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRKRGGYF
>Mature_304_residues
TSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKFPFPLVHIDTGHNFQEALDFRD
ELASKIGEKLIIRYVQDSIDQGKAVEEKGKFPSRNGIQTVTLLDTIAEFKFDACIGGARRDEEKARAKERVFSVRDEFGQ
WDPKLQRPELWNIYNGRISPGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDANDR
VEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRKRGGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=46.0264900662252, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35185; Mature: 35054

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKF
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCC
PFPLVHIDTGHNFQEALDFRDELASKIGEKLIIRYVQDSIDQGKAVEEKGKFPSRNGIQT
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCH
VTLLDTIAEFKFDACIGGARRDEEKARAKERVFSVRDEFGQWDPKLQRPELWNIYNGRIS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCC
PGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDAND
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCCEECEEEEEECCCC
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRK
CHHHHHHHHEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RGGYF
CCCCC
>Mature Secondary Structure 
TSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKF
CCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCC
PFPLVHIDTGHNFQEALDFRDELASKIGEKLIIRYVQDSIDQGKAVEEKGKFPSRNGIQT
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCH
VTLLDTIAEFKFDACIGGARRDEEKARAKERVFSVRDEFGQWDPKLQRPELWNIYNGRIS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCC
PGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDAND
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCCEECEEEEEECCCC
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRK
CHHHHHHHHEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RGGYF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA