Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 45659129

GI number: 45659129

Start: 4057523

End: 4058548

Strand: Reverse

Name: pheS

Synonym: LIC13310

Alternate gene names: 45659129

Gene position: 4058548-4057523 (Counterclockwise)

Preceding gene: 45659130

Following gene: 45659128

Centisome position: 94.89

GC content: 35.58

Gene sequence:

>1026_bases
ATGAACTTATCTGAAGAATTAGATTCTATCTATAAAGAAGCGATTCAAAAAATCGGTTCTTCCATATCCGAAGAAGATTT
AGATAAAAATAAAAACGATTTTATCGGAAAAAAAGGAAAGTTGACTGCGGTTTTAAAAAACGTTGCTTCACTTTCTATTG
AAGAAAAAAAAACAGTCGGACAAAAAGCAAACGAACTTTCTAAAAAATTAGAAACATTCATTTCTGAAACTAAAACTTCT
TTAAAGAAAAAGCTTTTTGAAAAACAGGCGGCTTCTGAGTTTTTTGATGTTCTTCGTCCTCTTTCTAAACCTTCTAACGG
AAGTTTACATCCAATCACTCAGATTCAATATGAAATAGAGGACATTTTTGCTTCTATGGGTTTTAGCGTGATGGATGGTC
CGGAAATTGAAACCGATACCAATAATTTTGGGGCCTTGAATTTTACAGAAGATCATCCAGCGAGAGAAATGCAAGATACG
TTTTATCTTGAAAATGGAAATCTTTTACGGACTCATACTTCCGCGATTCAAGTAAGAACATTAAGAAAATTGAAACCTCC
TTTTAGGATCATTGCACCCGGTAGAGTCTTTCGTTATGAGGAAGTAGACGCTTCTCACGAACATACTTTTTATCAGATCG
AAGGAATGGTCGTAGGTAAGGATATTTCTGCGGCAAACTTGATTGATACAATGCAGGTTTTACTTTCTAGAATTTTTGAA
AAAGAAATCAAAACTAGACTTAGGCCTGGTTACTTTCCTTTTGTGGAACCTGGCTTTGAATTGGATATAAGTTGTCTTGT
TTGTGATGGAAAAGGTTGCCCTGTTTGTAAACAATCTGGATGGCTGGAACTTTTGCCTTGTGGGCTCATTCATCCTAACG
TTCTATCTCACGCTGGATTGGATCCAAAAGAATGGACTGGTTTTGCGTTTGGTCTTGGGCTTGATAGACTCGTAATGATG
CGTTATGGGATTCACGACATTCGTTATTTTCAATCCGGAAACTTAAGATTTTTAAAACAATTCTAA

Upstream 100 bases:

>100_bases
TTCCACAATTTACGGGTCAAAAAATTCTAATCTTAGCCATGTCTAACGGGTCTTTCGGTGGTATTTATCCAAGGCTTGTT
GAATTGGCTCGGAAACACGT

Downstream 100 bases:

>100_bases
TATGATTTCTACACCCATTCAAACTCGATGGATGGACATGGACCCGTTTGCTCATGTAAGTAATTCGGTTTTTGTAGCTT
ATCTTGAAATTGGAAGAGTG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNLSEELDSIYKEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVASLSIEEKKTVGQKANELSKKLETFISETKTS
LKKKLFEKQAASEFFDVLRPLSKPSNGSLHPITQIQYEIEDIFASMGFSVMDGPEIETDTNNFGALNFTEDHPAREMQDT
FYLENGNLLRTHTSAIQVRTLRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
KEIKTRLRPGYFPFVEPGFELDISCLVCDGKGCPVCKQSGWLELLPCGLIHPNVLSHAGLDPKEWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF

Sequences:

>Translated_341_residues
MNLSEELDSIYKEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVASLSIEEKKTVGQKANELSKKLETFISETKTS
LKKKLFEKQAASEFFDVLRPLSKPSNGSLHPITQIQYEIEDIFASMGFSVMDGPEIETDTNNFGALNFTEDHPAREMQDT
FYLENGNLLRTHTSAIQVRTLRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
KEIKTRLRPGYFPFVEPGFELDISCLVCDGKGCPVCKQSGWLELLPCGLIHPNVLSHAGLDPKEWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF
>Mature_341_residues
MNLSEELDSIYKEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVASLSIEEKKTVGQKANELSKKLETFISETKTS
LKKKLFEKQAASEFFDVLRPLSKPSNGSLHPITQIQYEIEDIFASMGFSVMDGPEIETDTNNFGALNFTEDHPAREMQDT
FYLENGNLLRTHTSAIQVRTLRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
KEIKTRLRPGYFPFVEPGFELDISCLVCDGKGCPVCKQSGWLELLPCGLIHPNVLSHAGLDPKEWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=280, Percent_Identity=30, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=29.6296296296296, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788007, Length=341, Percent_Identity=41.0557184750733, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=32.4723247232472, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=32.4723247232472, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=32.5301204819277, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=31.3432835820896, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=314, Percent_Identity=25.796178343949, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI18858079, Length=280, Percent_Identity=30, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=28.0276816608997, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38627; Mature: 38627

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEELDSIYKEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVASLSIEEKKTVG
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHH
QKANELSKKLETFISETKTSLKKKLFEKQAASEFFDVLRPLSKPSNGSLHPITQIQYEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
DIFASMGFSVMDGPEIETDTNNFGALNFTEDHPAREMQDTFYLENGNLLRTHTSAIQVRT
HHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHEEEECCCEEEEHHHHHHHHH
LRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
HHHCCCCEEEECCCCEEEECCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHH
KEIKTRLRPGYFPFVEPGFELDISCLVCDGKGCPVCKQSGWLELLPCGLIHPNVLSHAGL
HHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCC
DPKEWTGFAFGLGLDRLVMMRYGIHDIRYFQSGNLRFLKQF
CHHHHCCCHHHHHHHHHHHHHHCHHHHHEEECCCEEEEECC
>Mature Secondary Structure
MNLSEELDSIYKEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVASLSIEEKKTVG
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHH
QKANELSKKLETFISETKTSLKKKLFEKQAASEFFDVLRPLSKPSNGSLHPITQIQYEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
DIFASMGFSVMDGPEIETDTNNFGALNFTEDHPAREMQDTFYLENGNLLRTHTSAIQVRT
HHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHEEEECCCEEEEHHHHHHHHH
LRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
HHHCCCCEEEECCCCEEEECCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHH
KEIKTRLRPGYFPFVEPGFELDISCLVCDGKGCPVCKQSGWLELLPCGLIHPNVLSHAGL
HHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCC
DPKEWTGFAFGLGLDRLVMMRYGIHDIRYFQSGNLRFLKQF
CHHHHCCCHHHHHHHHHHHHHHCHHHHHEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA