Click here to switch to the map view.

The map label for this gene is mpl

Identifier: 45659130

GI number: 45659130

Start: 4058545

End: 4059942

Strand: Reverse

Name: mpl

Synonym: LIC13311

Alternate gene names: 45659130

Gene position: 4059942-4058545 (Counterclockwise)

Preceding gene: 45659131

Following gene: 45659129

Centisome position: 94.92

GC content: 36.55

Gene sequence:

>1398_bases
TTGAAGATTTATTTGATCGGAATTGGTGGTATTGCGATGGGTAACCTCGCTTCTATGCTTCAAAAAAGTGGCTATGAGGT
TTCTGGTTCGGATGCGGGAGTTTATCCTCCTATGTCGGATAAATTGAAAGAATGGGGAATTCCGTATTTTGAAGGTTTTA
GAGCGGAGAATCTCAAGGGTCAAGATCTGATCATAGTAGGAAATGCGATTTCCAGAGGAAATCCGGAAGTAGAAGAGATG
CTTAATTTAGGAATGGATTATATTTCCATGCCCGCAGCAATTGGAAAATTTTTTCTCAAAGGGAAAAAAGTAATCGTAGT
CGCCGGGACTCACGGTAAGACCACGACTACATTCTTAATTCATCATATACTAAAAGAGAATGGAGTTGAACCCGGATTGT
TTGTGGGAGGGATTCGGAAAGATGGTCTACCCGGATTTGAATTGGGAAACGGGTCAATTTTCGTAATAGAAGGTGATGAA
TACGATACTGCGTTCTTTGATAAGTCCTCTAAGTTTTTACATTATAAACCCACCTACACGGTGTTAAATGCATTAGATTT
TGATCATGCAGATATTTTTCCGGACATTACGGCGATCGAGACCATGTTCAAACGTTTGATCAATCTAGTTCCTGGAAACG
GAAAAATTTTTTATTGGAATGGTTCTGGTTCTCTTAAAAAACTAGTTCAACACGTTTCGTTTACAAAGGTAGAAGGATTT
GAATGGAATCGCAAAGATTCTTGCCTTACTTGGAAAAAACACGAACTCTTTTGGGCAGATCAAAAGTTAAATCCGTCTTT
ATTTGGAGATCATAATTATAGAAATATAGAAGTTGCAATTCGAGTTTGTTCTGAAATTTTGAAAATTCAAAATCCTTCTG
GTTATAAAGAAGGGATTGCAAAGGCCATTGATTCTTTTCCGGGTGTTAAAAGAAGACAGGATATACTTTTCCAATCTCCG
AATGCGATCGTAATGGAGGATTTTGCACATCATCCGGTTGCAGTGCACGAAACAATTCATGCAGTTAAAAAAAGATTTCA
TGGTTATAAAATTATTTCCTTGTTCGAACCTAGAAGTGCGACCTCTCATCGAAATGTATTTCAAAAAGAATATTCATATT
CTTTTATAGGTTCCGATTTAACTATTCTTACTGAAATTCATAATCTAAAAAAAGTATCAAAGGATATTCGTCTGGATGTA
AAAAAGTTAGTTCAAAAACTTTTAAAAAACTCCAAAACGATTCCGGTTTATGCAAAGGATCCTAAAGAGTTACTCGTTAA
ACTCGAGAAAATGATTCCACAATTTACGGGTCAAAAAATTCTAATCTTAGCCATGTCTAACGGGTCTTTCGGTGGTATTT
ATCCAAGGCTTGTTGAATTGGCTCGGAAACACGTATGA

Upstream 100 bases:

>100_bases
ATAAGGAAATTCTAATATTCTTTTTATCCGGATCCGCCTATTTTTTAACTGGAATATTATTTTTAACGGTTATTTATAAT
TTAATCAGGACAATTCTCAA

Downstream 100 bases:

>100_bases
ACTTATCTGAAGAATTAGATTCTATCTATAAAGAAGCGATTCAAAAAATCGGTTCTTCCATATCCGAAGAAGATTTAGAT
AAAAATAAAAACGATTTTAT

Product: UDP-n-acetylmuramate:l-alanyl-gamma-d-glutamyl- meso- diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKIYLIGIGGIAMGNLASMLQKSGYEVSGSDAGVYPPMSDKLKEWGIPYFEGFRAENLKGQDLIIVGNAISRGNPEVEEM
LNLGMDYISMPAAIGKFFLKGKKVIVVAGTHGKTTTTFLIHHILKENGVEPGLFVGGIRKDGLPGFELGNGSIFVIEGDE
YDTAFFDKSSKFLHYKPTYTVLNALDFDHADIFPDITAIETMFKRLINLVPGNGKIFYWNGSGSLKKLVQHVSFTKVEGF
EWNRKDSCLTWKKHELFWADQKLNPSLFGDHNYRNIEVAIRVCSEILKIQNPSGYKEGIAKAIDSFPGVKRRQDILFQSP
NAIVMEDFAHHPVAVHETIHAVKKRFHGYKIISLFEPRSATSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDV
KKLVQKLLKNSKTIPVYAKDPKELLVKLEKMIPQFTGQKILILAMSNGSFGGIYPRLVELARKHV

Sequences:

>Translated_465_residues
MKIYLIGIGGIAMGNLASMLQKSGYEVSGSDAGVYPPMSDKLKEWGIPYFEGFRAENLKGQDLIIVGNAISRGNPEVEEM
LNLGMDYISMPAAIGKFFLKGKKVIVVAGTHGKTTTTFLIHHILKENGVEPGLFVGGIRKDGLPGFELGNGSIFVIEGDE
YDTAFFDKSSKFLHYKPTYTVLNALDFDHADIFPDITAIETMFKRLINLVPGNGKIFYWNGSGSLKKLVQHVSFTKVEGF
EWNRKDSCLTWKKHELFWADQKLNPSLFGDHNYRNIEVAIRVCSEILKIQNPSGYKEGIAKAIDSFPGVKRRQDILFQSP
NAIVMEDFAHHPVAVHETIHAVKKRFHGYKIISLFEPRSATSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDV
KKLVQKLLKNSKTIPVYAKDPKELLVKLEKMIPQFTGQKILILAMSNGSFGGIYPRLVELARKHV
>Mature_465_residues
MKIYLIGIGGIAMGNLASMLQKSGYEVSGSDAGVYPPMSDKLKEWGIPYFEGFRAENLKGQDLIIVGNAISRGNPEVEEM
LNLGMDYISMPAAIGKFFLKGKKVIVVAGTHGKTTTTFLIHHILKENGVEPGLFVGGIRKDGLPGFELGNGSIFVIEGDE
YDTAFFDKSSKFLHYKPTYTVLNALDFDHADIFPDITAIETMFKRLINLVPGNGKIFYWNGSGSLKKLVQHVSFTKVEGF
EWNRKDSCLTWKKHELFWADQKLNPSLFGDHNYRNIEVAIRVCSEILKIQNPSGYKEGIAKAIDSFPGVKRRQDILFQSP
NAIVMEDFAHHPVAVHETIHAVKKRFHGYKIISLFEPRSATSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDV
KKLVQKLLKNSKTIPVYAKDPKELLVKLEKMIPQFTGQKILILAMSNGSFGGIYPRLVELARKHV

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=473, Percent_Identity=35.3065539112051, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1786279, Length=468, Percent_Identity=25.2136752136752, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 52286; Mature: 52286

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLIGIGGIAMGNLASMLQKSGYEVSGSDAGVYPPMSDKLKEWGIPYFEGFRAENLKG
CEEEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCCC
QDLIIVGNAISRGNPEVEEMLNLGMDYISMPAAIGKFFLKGKKVIVVAGTHGKTTTTFLI
CEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
HHILKENGVEPGLFVGGIRKDGLPGFELGNGSIFVIEGDEYDTAFFDKSSKFLHYKPTYT
HHHHHHCCCCCCEEECCCCCCCCCCEEECCCEEEEEECCCCCCHHCCCCCCEEEECCHHH
VLNALDFDHADIFPDITAIETMFKRLINLVPGNGKIFYWNGSGSLKKLVQHVSFTKVEGF
HHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCHHEECCC
EWNRKDSCLTWKKHELFWADQKLNPSLFGDHNYRNIEVAIRVCSEILKIQNPSGYKEGIA
CCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
KAIDSFPGVKRRQDILFQSPNAIVMEDFAHHPVAVHETIHAVKKRFHGYKIISLFEPRSA
HHHHHCCCCCHHHHHHEECCCEEEEHHCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCC
TSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDVKKLVQKLLKNSKTIPVYAKD
CHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PKELLVKLEKMIPQFTGQKILILAMSNGSFGGIYPRLVELARKHV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIYLIGIGGIAMGNLASMLQKSGYEVSGSDAGVYPPMSDKLKEWGIPYFEGFRAENLKG
CEEEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCCC
QDLIIVGNAISRGNPEVEEMLNLGMDYISMPAAIGKFFLKGKKVIVVAGTHGKTTTTFLI
CEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
HHILKENGVEPGLFVGGIRKDGLPGFELGNGSIFVIEGDEYDTAFFDKSSKFLHYKPTYT
HHHHHHCCCCCCEEECCCCCCCCCCEEECCCEEEEEECCCCCCHHCCCCCCEEEECCHHH
VLNALDFDHADIFPDITAIETMFKRLINLVPGNGKIFYWNGSGSLKKLVQHVSFTKVEGF
HHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCHHEECCC
EWNRKDSCLTWKKHELFWADQKLNPSLFGDHNYRNIEVAIRVCSEILKIQNPSGYKEGIA
CCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
KAIDSFPGVKRRQDILFQSPNAIVMEDFAHHPVAVHETIHAVKKRFHGYKIISLFEPRSA
HHHHHCCCCCHHHHHHEECCCEEEEHHCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCC
TSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDVKKLVQKLLKNSKTIPVYAKD
CHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PKELLVKLEKMIPQFTGQKILILAMSNGSFGGIYPRLVELARKHV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]