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The map label for this gene is priA

Identifier: 45657424

GI number: 45657424

Start: 1913874

End: 1915817

Strand: Direct

Name: priA

Synonym: LIC11551

Alternate gene names: 45657424

Gene position: 1913874-1915817 (Clockwise)

Preceding gene: 45657423

Following gene: 45657425

Centisome position: 44.75

GC content: 36.78

Gene sequence:

>1944_bases
TTGATTTATTACGCAGAAGTTGCGTTCGATCTTCCAATTGAAGAGGACACTTTTACATATGAGATTCCACCTAACGTTCA
GATAGGTGTTCGAGTTTTGGTAAAACTACGCAATCGGGAAGAGGAAGGGATTATAGTTTCGATTCATCAAAACGAACCAA
ATTATAAAGTTTTTCAAATAGAAAAAATTATAGATAAAACTCCGATTGTTCTTCAAGAACAAATCGATTTAGCTCATTGG
ATGAAAAATCAATACATTGCTTCTCTCGGAGAATGTATTTATAAAATGATTCCTGCAGGAAGAAGGCAGGTAAAGCTCGA
AACTTTTCCTTCGGACGCAGAAGGTAAACCAGTTACTTTAAACGAAGAACAACAAATCGCAACTCAAAATATACTTTCTA
CTTTCGGCACCGCCGCAGTTCATTTGTTATTCGGAATTACTGGCTCCGGAAAAACAGAAGTTTATATCCATCTCATACAA
AAGGTTCTGGAAACTCCAAATCGTTCTGTCATTTTACTTGTTCCCGAAATCAGTTTAACATTTCATATTATTCGAAAGTT
AGAACTTATTTTTCCGGGACAATTGGCGGTACTTCATTCGGCTCTAAAGGTTTCCGAAAAGTTTAAGGCATATAACGAAC
TTCTCACAGGTAAAAAAAGAATTGCCGTCGGAACTAGAAGCGCTGTATTTGCGCCTGTTTCTAACTTAGGACTTGTGATT
ATAGACGAGGATCATGATTCTTCTTTTAAAGAACATTCTAGTCCTAGATATCACGCAAGGCAAATCGCCTTACAGAGATG
TAGGACTAATGAAGCTGTTCTTGTGATGGGAACCGCGACACCTTCTTTAGAAATTTATCATCTTGCAAAAGAGGGGAAAA
TTCATCTTCATACTTTGACTAAAAGACCTCAAGGAGTGTTACCGCCTACGGTTCGAATCGTGGAGAACCAAAAAGAGTCT
AACGTTCTTAGCTCAGAACTTTCCTTTGCGATCAAACAAAGATTAGAGAAAAAAGAACAGATCATACTACTTTTAAACAG
AAGAGGTTATAGTCCTCTGATCTATTCTCCGTCCACTTCTTCTTATGTGCCTTGTCCAAATTGTACGACTAATCTTTGTT
ATCATAAAAAAGGAACTACGATTTGTCATCTCTGTGGGCATACGGAAACTTTAGATTCCTTGCAAAAAAGAATGGGAGAA
GGTCTAACCCTCAAGGGAACCGGAACACAAAAACTAGAAGAAAATCTATTAGAAGCGTTTCCGCAAACTAGGGTAGAAAG
ACTTGATCAAGATTCTATTCAAGATCGTAGTTTATTAAACGAAGTCATATCTCGTCTTTTAGGAGGAGAAATTGATATTC
TTACTGGAACTCAGATGATCGCAAAAGGATTGGATGCCTCTAGAGTTACATTAGTCGGTGTTTTAAATGCAGGAATAGGG
CTTGGACTTCCAGATTTTAGAGCCAACGAAAGAGTATTTTCTCTTTTAACTCAAGTGGCTGGAAGGGCGGGACGCTCTAA
GTTAAAAGGGGAAGTGTTGATAGAAACTAACGCACCTAATCATCCGGTCATTCAAATGGCAATGAACCAAAACTATATTC
AATTTTATGAATCTGAAATTCCGGTTCGAAAGGAACTTTTTTATCCTCCATTTTCTAGACTAGTTCGAGTTGTTTCCCGT
TCTAAGGAAGAACAGATTTCACTTGAAACAATCGAGTTAGTATTCGGGGTTCTAAAAAAATTTTTTCCCTCCAAAGATAC
AATTCTACTCGGACCGGCTCCTTGTCCTTTTTATAGAATTGATTCCAATTTTAGAAATCATATCATTCTCAAAACTTCTT
CGCCAAACATTTGGAGAGAAATTTTTAAAAAAGAAATTCGCCCTTTAAAACTATCTAAAAAAGTGTATTTAGAAATAGAT
TTTGACCCTTTGGATTTGGTATAA

Upstream 100 bases:

>100_bases
ATTCATTTGAATTCGAGACGATCAAAAAAGAAGAAATTTCTATCAAAGAAAGTATCCTTTCTCAATTGGCGATCTTTCAA
AACAGACTCAAGGATGTACT

Downstream 100 bases:

>100_bases
GAATGCGCCATCTTTACTTCGCACAGAGCCTTGCTACGGTTATCTCGCTTATAGCTCGATAGGGACAGAACCTTGCAGCT
TATTTTTTTGAGAAGATAAA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MIYYAEVAFDLPIEEDTFTYEIPPNVQIGVRVLVKLRNREEEGIIVSIHQNEPNYKVFQIEKIIDKTPIVLQEQIDLAHW
MKNQYIASLGECIYKMIPAGRRQVKLETFPSDAEGKPVTLNEEQQIATQNILSTFGTAAVHLLFGITGSGKTEVYIHLIQ
KVLETPNRSVILLVPEISLTFHIIRKLELIFPGQLAVLHSALKVSEKFKAYNELLTGKKRIAVGTRSAVFAPVSNLGLVI
IDEDHDSSFKEHSSPRYHARQIALQRCRTNEAVLVMGTATPSLEIYHLAKEGKIHLHTLTKRPQGVLPPTVRIVENQKES
NVLSSELSFAIKQRLEKKEQIILLLNRRGYSPLIYSPSTSSYVPCPNCTTNLCYHKKGTTICHLCGHTETLDSLQKRMGE
GLTLKGTGTQKLEENLLEAFPQTRVERLDQDSIQDRSLLNEVISRLLGGEIDILTGTQMIAKGLDASRVTLVGVLNAGIG
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMAMNQNYIQFYESEIPVRKELFYPPFSRLVRVVSR
SKEEQISLETIELVFGVLKKFFPSKDTILLGPAPCPFYRIDSNFRNHIILKTSSPNIWREIFKKEIRPLKLSKKVYLEID
FDPLDLV

Sequences:

>Translated_647_residues
MIYYAEVAFDLPIEEDTFTYEIPPNVQIGVRVLVKLRNREEEGIIVSIHQNEPNYKVFQIEKIIDKTPIVLQEQIDLAHW
MKNQYIASLGECIYKMIPAGRRQVKLETFPSDAEGKPVTLNEEQQIATQNILSTFGTAAVHLLFGITGSGKTEVYIHLIQ
KVLETPNRSVILLVPEISLTFHIIRKLELIFPGQLAVLHSALKVSEKFKAYNELLTGKKRIAVGTRSAVFAPVSNLGLVI
IDEDHDSSFKEHSSPRYHARQIALQRCRTNEAVLVMGTATPSLEIYHLAKEGKIHLHTLTKRPQGVLPPTVRIVENQKES
NVLSSELSFAIKQRLEKKEQIILLLNRRGYSPLIYSPSTSSYVPCPNCTTNLCYHKKGTTICHLCGHTETLDSLQKRMGE
GLTLKGTGTQKLEENLLEAFPQTRVERLDQDSIQDRSLLNEVISRLLGGEIDILTGTQMIAKGLDASRVTLVGVLNAGIG
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMAMNQNYIQFYESEIPVRKELFYPPFSRLVRVVSR
SKEEQISLETIELVFGVLKKFFPSKDTILLGPAPCPFYRIDSNFRNHIILKTSSPNIWREIFKKEIRPLKLSKKVYLEID
FDPLDLV
>Mature_647_residues
MIYYAEVAFDLPIEEDTFTYEIPPNVQIGVRVLVKLRNREEEGIIVSIHQNEPNYKVFQIEKIIDKTPIVLQEQIDLAHW
MKNQYIASLGECIYKMIPAGRRQVKLETFPSDAEGKPVTLNEEQQIATQNILSTFGTAAVHLLFGITGSGKTEVYIHLIQ
KVLETPNRSVILLVPEISLTFHIIRKLELIFPGQLAVLHSALKVSEKFKAYNELLTGKKRIAVGTRSAVFAPVSNLGLVI
IDEDHDSSFKEHSSPRYHARQIALQRCRTNEAVLVMGTATPSLEIYHLAKEGKIHLHTLTKRPQGVLPPTVRIVENQKES
NVLSSELSFAIKQRLEKKEQIILLLNRRGYSPLIYSPSTSSYVPCPNCTTNLCYHKKGTTICHLCGHTETLDSLQKRMGE
GLTLKGTGTQKLEENLLEAFPQTRVERLDQDSIQDRSLLNEVISRLLGGEIDILTGTQMIAKGLDASRVTLVGVLNAGIG
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMAMNQNYIQFYESEIPVRKELFYPPFSRLVRVVSR
SKEEQISLETIELVFGVLKKFFPSKDTILLGPAPCPFYRIDSNFRNHIILKTSSPNIWREIFKKEIRPLKLSKKVYLEID
FDPLDLV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=539, Percent_Identity=33.5807050092764, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 73092; Mature: 73092

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYYAEVAFDLPIEEDTFTYEIPPNVQIGVRVLVKLRNREEEGIIVSIHQNEPNYKVFQI
CEEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEE
EKIIDKTPIVLQEQIDLAHWMKNQYIASLGECIYKMIPAGRRQVKLETFPSDAEGKPVTL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEE
NEEQQIATQNILSTFGTAAVHLLFGITGSGKTEVYIHLIQKVLETPNRSVILLVPEISLT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHH
FHIIRKLELIFPGQLAVLHSALKVSEKFKAYNELLTGKKRIAVGTRSAVFAPVSNLGLVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCEEE
IDEDHDSSFKEHSSPRYHARQIALQRCRTNEAVLVMGTATPSLEIYHLAKEGKIHLHTLT
EECCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEEEC
KRPQGVLPPTVRIVENQKESNVLSSELSFAIKQRLEKKEQIILLLNRRGYSPLIYSPSTS
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCC
SYVPCPNCTTNLCYHKKGTTICHLCGHTETLDSLQKRMGEGLTLKGTGTQKLEENLLEAF
CCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHC
PQTRVERLDQDSIQDRSLLNEVISRLLGGEIDILTGTQMIAKGLDASRVTLVGVLNAGIG
HHHHHHHCCHHCHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCEEEEEEECCCCC
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMAMNQNYIQFYESEI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHCCC
PVRKELFYPPFSRLVRVVSRSKEEQISLETIELVFGVLKKFFPSKDTILLGPAPCPFYRI
CHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
DSNFRNHIILKTSSPNIWREIFKKEIRPLKLSKKVYLEIDFDPLDLV
CCCCCCEEEEEECCCHHHHHHHHHCCCCEEECCEEEEEECCCHHCCC
>Mature Secondary Structure
MIYYAEVAFDLPIEEDTFTYEIPPNVQIGVRVLVKLRNREEEGIIVSIHQNEPNYKVFQI
CEEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEE
EKIIDKTPIVLQEQIDLAHWMKNQYIASLGECIYKMIPAGRRQVKLETFPSDAEGKPVTL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEE
NEEQQIATQNILSTFGTAAVHLLFGITGSGKTEVYIHLIQKVLETPNRSVILLVPEISLT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHH
FHIIRKLELIFPGQLAVLHSALKVSEKFKAYNELLTGKKRIAVGTRSAVFAPVSNLGLVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCEEE
IDEDHDSSFKEHSSPRYHARQIALQRCRTNEAVLVMGTATPSLEIYHLAKEGKIHLHTLT
EECCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEEEC
KRPQGVLPPTVRIVENQKESNVLSSELSFAIKQRLEKKEQIILLLNRRGYSPLIYSPSTS
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCC
SYVPCPNCTTNLCYHKKGTTICHLCGHTETLDSLQKRMGEGLTLKGTGTQKLEENLLEAF
CCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHC
PQTRVERLDQDSIQDRSLLNEVISRLLGGEIDILTGTQMIAKGLDASRVTLVGVLNAGIG
HHHHHHHCCHHCHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCEEEEEEECCCCC
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMAMNQNYIQFYESEI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHCCC
PVRKELFYPPFSRLVRVVSRSKEEQISLETIELVFGVLKKFFPSKDTILLGPAPCPFYRI
CHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
DSNFRNHIILKTSSPNIWREIFKKEIRPLKLSKKVYLEIDFDPLDLV
CCCCCCEEEEEECCCHHHHHHHHHCCCCEEECCEEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]