Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 45657422

GI number: 45657422

Start: 1911687

End: 1913033

Strand: Direct

Name: ntrX [H]

Synonym: LIC11549

Alternate gene names: 45657422

Gene position: 1911687-1913033 (Clockwise)

Preceding gene: 45657421

Following gene: 45657423

Centisome position: 44.69

GC content: 35.78

Gene sequence:

>1347_bases
ATGAAAATTTTTATCGCAGACGATGAACCAGAAATCAGAAAATCTCTTAAAGAAATTTTAGAGGACGAAAATTTCGAAGT
AGAAACTTTCGCTACTGGTAAAACCTTACTCAAACAACTCAAAAATGATAGACCTTCTCTAATTCTTTTGGACGTATGGT
TAGGCAAAGAAGATGGGATTACCATTTTAGAAGAGTGTAAAAAAATCTATCCAACTCTTCCTATACTTATGATTTCGGGT
CACGGAACGATAGAAATTGCTGTGGCTGCTACAAAAAAAGGAGCTGTGGATTTTTTAGAAAAACCTCTTTCGATCGAAAA
AGTTTTACAAGCGATAGATGATCTAGTCAAGTCTGATCAAAAAAATTCGGCTTCCGATATTAAATTAGAATATGATGAAA
TTCTTGGAAACTCCCCCGCCATTCAAAAAGTAAAGTTCGCAATCGCTCAAGCTGCGTCTACTAACGCTCGAGTTTTTATC
TACGGAGAAAACGGAACCGGAAAAGAACTCGTTGCAAAAACTATATTCAAAAATTCTAAACGAAAAGATTCTCCTTTTGT
AGAGATGAACTGTGCTGCAATTCCGGAAGAACTTATAGAGTCTGAGTTATTTGGATTTACAAAAGGAGCTTTTACGGGCG
CGACCGATTCTAGAATCGGAAAATTCGAAGCCGCCAACGGAGGCACTTTGTTTCTGGACGAAATTTGTGACATGTCACTT
TCTACTCAGGCGAAAGTATTACGAATTCTTCAAGAGCAAAGATTTGAAAAATTGGGAAGTACAGAGACGATCACAGTAGA
CGTTAGAATTATTGCAGCTACAAATATTCCAGTAGAAGAGGCGATTCGAGACGGAAAGTTTAGAGAAGACTTATATTATA
GATTGAATGTGATTCCAATAACAATTCCACCTCTTAGAGAAAGAACTTCCGATATTCCATTGCTTGTGGATTATTATATT
TCCAAAACCTTAGAAGAAAATAATTTACTTTCTAAAAAAATAGAATCTGAAGCGGTTTCTATCTTACAAAATCATTTTTG
GCCAGGTAACATTCGGGAACTAAAAAATGTAATGGAAAGGCTTTGTATTATGACCGTAGGTTCCGTGATTACTGCAAACG
ACGCAAGAGACGCACTCAAAGGTTTTAAAACTGCAAACGAAATGGTGGAGTTAGGAGATTTTAGAAAAGCTAAAGAAGAA
TTTGAAAGACAGTATATTATAAAAACATTACAAACAAATGAGGGAAACGTGACCAGAACTTCCCGAGTTTTAGGAATTGA
ACGTTCTCACTTATACAGAAAAATGAAGTCGCTTAATATTTCTTCGGATCAGTATACAGATGGATAA

Upstream 100 bases:

>100_bases
CAAAAAAGTGTGATTGACCACAACGGTCATATCTCTGTAGATTCTTCCAGTATGGGAGGATGTAGTTTTCAAATAGAACT
TCCAGTGTCTTAAAACGATC

Downstream 100 bases:

>100_bases
AAAAATTCTATCCAAACTTGGAAGAGTATTAGGTGATCTCCGTTTTTTGATCCAAAAAAAACAGATCCCAATCTTACAAT
TTTCTGGAGAAAAACCTCCT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKIFIADDEPEIRKSLKEILEDENFEVETFATGKTLLKQLKNDRPSLILLDVWLGKEDGITILEECKKIYPTLPILMISG
HGTIEIAVAATKKGAVDFLEKPLSIEKVLQAIDDLVKSDQKNSASDIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFI
YGENGTGKELVAKTIFKNSKRKDSPFVEMNCAAIPEELIESELFGFTKGAFTGATDSRIGKFEAANGGTLFLDEICDMSL
STQAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIRDGKFREDLYYRLNVIPITIPPLRERTSDIPLLVDYYI
SKTLEENNLLSKKIESEAVSILQNHFWPGNIRELKNVMERLCIMTVGSVITANDARDALKGFKTANEMVELGDFRKAKEE
FERQYIIKTLQTNEGNVTRTSRVLGIERSHLYRKMKSLNISSDQYTDG

Sequences:

>Translated_448_residues
MKIFIADDEPEIRKSLKEILEDENFEVETFATGKTLLKQLKNDRPSLILLDVWLGKEDGITILEECKKIYPTLPILMISG
HGTIEIAVAATKKGAVDFLEKPLSIEKVLQAIDDLVKSDQKNSASDIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFI
YGENGTGKELVAKTIFKNSKRKDSPFVEMNCAAIPEELIESELFGFTKGAFTGATDSRIGKFEAANGGTLFLDEICDMSL
STQAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIRDGKFREDLYYRLNVIPITIPPLRERTSDIPLLVDYYI
SKTLEENNLLSKKIESEAVSILQNHFWPGNIRELKNVMERLCIMTVGSVITANDARDALKGFKTANEMVELGDFRKAKEE
FERQYIIKTLQTNEGNVTRTSRVLGIERSHLYRKMKSLNISSDQYTDG
>Mature_448_residues
MKIFIADDEPEIRKSLKEILEDENFEVETFATGKTLLKQLKNDRPSLILLDVWLGKEDGITILEECKKIYPTLPILMISG
HGTIEIAVAATKKGAVDFLEKPLSIEKVLQAIDDLVKSDQKNSASDIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFI
YGENGTGKELVAKTIFKNSKRKDSPFVEMNCAAIPEELIESELFGFTKGAFTGATDSRIGKFEAANGGTLFLDEICDMSL
STQAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIRDGKFREDLYYRLNVIPITIPPLRERTSDIPLLVDYYI
SKTLEENNLLSKKIESEAVSILQNHFWPGNIRELKNVMERLCIMTVGSVITANDARDALKGFKTANEMVELGDFRKAKEE
FERQYIIKTLQTNEGNVTRTSRVLGIERSHLYRKMKSLNISSDQYTDG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=40.5286343612335, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=38.5135135135135, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI1790299, Length=463, Percent_Identity=35.4211663066955, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=38.0410022779043, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=42.5, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=42.9429429429429, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=43.217665615142, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=39.4321766561514, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=36.8263473053892, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=36.4779874213836, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1787583, Length=350, Percent_Identity=32, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=30.976430976431, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=35.1694915254237, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50346; Mature: 50346

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFIADDEPEIRKSLKEILEDENFEVETFATGKTLLKQLKNDRPSLILLDVWLGKEDGI
CEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCC
TILEECKKIYPTLPILMISGHGTIEIAVAATKKGAVDFLEKPLSIEKVLQAIDDLVKSDQ
HHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHCCC
KNSASDIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYGENGTGKELVAKTIFKNSK
CCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCC
RKDSPFVEMNCAAIPEELIESELFGFTKGAFTGATDSRIGKFEAANGGTLFLDEICDMSL
CCCCCCEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEHHHHCCCCC
STQAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIRDGKFREDLYYRLNVIPI
CHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHCCEEEEEEEEE
TIPPLRERTSDIPLLVDYYISKTLEENNLLSKKIESEAVSILQNHFWPGNIRELKNVMER
ECCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LCIMTVGSVITANDARDALKGFKTANEMVELGDFRKAKEEFERQYIIKTLQTNEGNVTRT
HHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
SRVLGIERSHLYRKMKSLNISSDQYTDG
HHHHCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIFIADDEPEIRKSLKEILEDENFEVETFATGKTLLKQLKNDRPSLILLDVWLGKEDGI
CEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCC
TILEECKKIYPTLPILMISGHGTIEIAVAATKKGAVDFLEKPLSIEKVLQAIDDLVKSDQ
HHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHCCC
KNSASDIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYGENGTGKELVAKTIFKNSK
CCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCC
RKDSPFVEMNCAAIPEELIESELFGFTKGAFTGATDSRIGKFEAANGGTLFLDEICDMSL
CCCCCCEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEHHHHCCCCC
STQAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIRDGKFREDLYYRLNVIPI
CHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHCCEEEEEEEEE
TIPPLRERTSDIPLLVDYYISKTLEENNLLSKKIESEAVSILQNHFWPGNIRELKNVMER
ECCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LCIMTVGSVITANDARDALKGFKTANEMVELGDFRKAKEEFERQYIIKTLQTNEGNVTRT
HHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
SRVLGIERSHLYRKMKSLNISSDQYTDG
HHHHCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]