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The map label for this gene is rsbW [H]

Identifier: 45657355

GI number: 45657355

Start: 1829149

End: 1829577

Strand: Direct

Name: rsbW [H]

Synonym: LIC11480

Alternate gene names: 45657355

Gene position: 1829149-1829577 (Clockwise)

Preceding gene: 45657354

Following gene: 45657356

Centisome position: 42.77

GC content: 33.8

Gene sequence:

>429_bases
ATGGATTCTGGAAAAAAGAAAAATTTAGATAATCTGTTTCGGTTGCAGATTCCTTCTCATCCACGTTATTTGTCCATTGT
TCGGAGTTTGGTCTATAATTTGGCATTTGAGAATGGATTTACGGCGAGTGATTCCGCAGACCTAAAACTCGCAGTAGGAG
AATGTCTTCTCAACGTTATCAAACATTCTTACGAAGGTAAAAAAAATTTACCTATTTTTTTGGAAATTAGTCTTTTTGAT
AATCGTATAGAAATTAGAATTCGGGATTTTGGAAATCAGAAAAATCTTTCCGAAATGAGAGGATATGAACCAAGTGATTA
TCGGGAGGAAGGAATCGGTCTTTATCTTGTAAAAAAACTTACAGATCATTTTTATCTAGATCAATCCTTGCCGGAAGGAA
ATAGGCTGATTCTTACAAAATTAAAATGA

Upstream 100 bases:

>100_bases
ACTTACTTTTTGTGGTAATCTTTGATAGACTATACGGCGATCGAATTTTTGCGACGTATAACAAAATATTAGTTTATTTT
ATTATAAAAATTGAATCAAA

Downstream 100 bases:

>100_bases
ATATTTTATCTAAAATCATAATGCTGTTTTTTATTTTTGTTTCAATTTTTACTTGTAGAAAAGGTCCTTCCATTTCTAAA
GAAGAAGTTCAAAAACTCAG

Product: anti-sigma factor antagonist

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW [H]

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MDSGKKKNLDNLFRLQIPSHPRYLSIVRSLVYNLAFENGFTASDSADLKLAVGECLLNVIKHSYEGKKNLPIFLEISLFD
NRIEIRIRDFGNQKNLSEMRGYEPSDYREEGIGLYLVKKLTDHFYLDQSLPEGNRLILTKLK

Sequences:

>Translated_142_residues
MDSGKKKNLDNLFRLQIPSHPRYLSIVRSLVYNLAFENGFTASDSADLKLAVGECLLNVIKHSYEGKKNLPIFLEISLFD
NRIEIRIRDFGNQKNLSEMRGYEPSDYREEGIGLYLVKKLTDHFYLDQSLPEGNRLILTKLK
>Mature_142_residues
MDSGKKKNLDNLFRLQIPSHPRYLSIVRSLVYNLAFENGFTASDSADLKLAVGECLLNVIKHSYEGKKNLPIFLEISLFD
NRIEIRIRDFGNQKNLSEMRGYEPSDYREEGIGLYLVKKLTDHFYLDQSLPEGNRLILTKLK

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010193
- InterPro:   IPR003594 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 16425; Mature: 16425

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGKKKNLDNLFRLQIPSHPRYLSIVRSLVYNLAFENGFTASDSADLKLAVGECLLNVI
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHH
KHSYEGKKNLPIFLEISLFDNRIEIRIRDFGNQKNLSEMRGYEPSDYREEGIGLYLVKKL
HHHCCCCCCCCEEEEEEEECCEEEEEEEECCCCCCHHHHCCCCCCCHHHCCCHHHHHHHH
TDHFYLDQSLPEGNRLILTKLK
HHHHEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MDSGKKKNLDNLFRLQIPSHPRYLSIVRSLVYNLAFENGFTASDSADLKLAVGECLLNVI
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHH
KHSYEGKKNLPIFLEISLFDNRIEIRIRDFGNQKNLSEMRGYEPSDYREEGIGLYLVKKL
HHHCCCCCCCCEEEEEEEECCEEEEEEEECCCCCCHHHHCCCCCCCHHHCCCHHHHHHHH
TDHFYLDQSLPEGNRLILTKLK
HHHHEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]