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The map label for this gene is hisF

Identifier: 45657337

GI number: 45657337

Start: 1803771

End: 1804541

Strand: Direct

Name: hisF

Synonym: LIC11462

Alternate gene names: 45657337

Gene position: 1803771-1804541 (Clockwise)

Preceding gene: 45657336

Following gene: 45657339

Centisome position: 42.17

GC content: 40.34

Gene sequence:

>771_bases
ATGAGTAATCTTACTGCCAGAGTAATTCCCTGTCTGGACATCAAAGACGGGCGTGTAGTTAAGGGAGTAAATTTTGTAAA
TCTAGTGGACGCTGGAGATCCAGTGGAATCGGCTGCGATCTACGAAGAGAATTTAGCCGATGAACTTTGTTTTTTAGATA
TAACCGCTTCTTCGGATAGAAGAGAGATATTACTTCATCTTGTAGAGAGGATAGCCGAAAAGATTTTCATTCCTTTTACA
GTAGGAGGAGGAATTAGAACCGTAGACGACGTAAAAGCCGTTTTAGAAAAAGGAGCGGACAAGGTATCAATCAATACTGC
AGCTTTTCAAAACCCCAAATTGTTAACATATTCTTCCGAAATTTATGGATCTCAATGTATCGTTTGTGCAATTGATGTTA
AGCATGAAAAAGAAAGAGATCGATACGAAGTTTTTTTACACGGAGGAAGAACAGCAACCGGAAGGGAGGCTTTGGATTGG
GCTCAAGAGGCCGCCGAAAAAGGTGCTGGAGAAATTTTACTTACGTCTATGGATCGAGATGGAACTAGAAATGGATTTGA
TATTCATCTTCTAAAGAATTTTTCCTCGTCTCTGGGAATTCCAATTATAGCATCTGGAGGGGCCGGAAATCCGGAGCATA
TGGTGGAAGCCATTTTACGCGGGAAAGCAGATGCAGTACTTGCTGCTTCTATTTTTCATTTTGGAGAATATTCTATCCGT
GAGACTAAAAAAGCTATGGAAGAGATGGGTATTTCGGTTCGTTTAGATTAA

Upstream 100 bases:

>100_bases
AAATTACTACTCCTCATTTTCAAGAAGGAAAACTTTTCGGAATTGCATTAGCACTTTCTACATTAGAAGGAATGAATATT
CAGTTTCCGGAAAGTATTAA

Downstream 100 bases:

>100_bases
AATGATATTTGTCTATAGATGCTGAAAATGGTTTTCTATTTTTGGAATGTGATAGTTTTTGTTAGGCAATATTATTTTAA
CTTGAGTTCGACGTAAGAAT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSNLTARVIPCLDIKDGRVVKGVNFVNLVDAGDPVESAAIYEENLADELCFLDITASSDRREILLHLVERIAEKIFIPFT
VGGGIRTVDDVKAVLEKGADKVSINTAAFQNPKLLTYSSEIYGSQCIVCAIDVKHEKERDRYEVFLHGGRTATGREALDW
AQEAAEKGAGEILLTSMDRDGTRNGFDIHLLKNFSSSLGIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
ETKKAMEEMGISVRLD

Sequences:

>Translated_256_residues
MSNLTARVIPCLDIKDGRVVKGVNFVNLVDAGDPVESAAIYEENLADELCFLDITASSDRREILLHLVERIAEKIFIPFT
VGGGIRTVDDVKAVLEKGADKVSINTAAFQNPKLLTYSSEIYGSQCIVCAIDVKHEKERDRYEVFLHGGRTATGREALDW
AQEAAEKGAGEILLTSMDRDGTRNGFDIHLLKNFSSSLGIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
ETKKAMEEMGISVRLD
>Mature_255_residues
SNLTARVIPCLDIKDGRVVKGVNFVNLVDAGDPVESAAIYEENLADELCFLDITASSDRREILLHLVERIAEKIFIPFTV
GGGIRTVDDVKAVLEKGADKVSINTAAFQNPKLLTYSSEIYGSQCIVCAIDVKHEKERDRYEVFLHGGRTATGREALDWA
QEAAEKGAGEILLTSMDRDGTRNGFDIHLLKNFSSSLGIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIRE
TKKAMEEMGISVRLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=46.484375, Blast_Score=220, Evalue=6e-59,
Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=26.5560165975104, Blast_Score=81, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=32.0634920634921, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS61_LEPIN (P59119)

Other databases:

- EMBL:   AE010300
- RefSeq:   NP_712687.1
- ProteinModelPortal:   P59119
- SMR:   P59119
- GeneID:   1151849
- GenomeReviews:   AE010300_GR
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   LINT-130-01:LINT-130-01-001423-MONOMER
- BioCyc:   LINT189518:LA2506-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27898; Mature: 27767

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLTARVIPCLDIKDGRVVKGVNFVNLVDAGDPVESAAIYEENLADELCFLDITASSDR
CCCCCEEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCH
REILLHLVERIAEKIFIPFTVGGGIRTVDDVKAVLEKGADKVSINTAAFQNPKLLTYSSE
HHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCEEEEEEHH
IYGSQCIVCAIDVKHEKERDRYEVFLHGGRTATGREALDWAQEAAEKGAGEILLTSMDRD
HCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCC
GTRNGFDIHLLKNFSSSLGIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
CCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
ETKKAMEEMGISVRLD
HHHHHHHHCCCEEEEC
>Mature Secondary Structure 
SNLTARVIPCLDIKDGRVVKGVNFVNLVDAGDPVESAAIYEENLADELCFLDITASSDR
CCCCEEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCH
REILLHLVERIAEKIFIPFTVGGGIRTVDDVKAVLEKGADKVSINTAAFQNPKLLTYSSE
HHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCEEEEEEHH
IYGSQCIVCAIDVKHEKERDRYEVFLHGGRTATGREALDWAQEAAEKGAGEILLTSMDRD
HCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCC
GTRNGFDIHLLKNFSSSLGIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
CCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
ETKKAMEEMGISVRLD
HHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204