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The map label for this gene is gatA

Identifier: 45657336

GI number: 45657336

Start: 1802311

End: 1803774

Strand: Direct

Name: gatA

Synonym: LIC11461

Alternate gene names: 45657336

Gene position: 1802311-1803774 (Clockwise)

Preceding gene: 45657335

Following gene: 45657337

Centisome position: 42.14

GC content: 38.25

Gene sequence:

>1464_bases
ATGAACGAAATATTAAAAAAACCTTATGTGGAATTGAAAGATTCTCTGAATTCCGGAAAAATATCTTCCACAGAACTTGT
AAGCGCTTGTATCAATCGGATTCGAGAAGTAGACGGTGCTGTAAAAGCGTTTCTATACTTAGATGAAAAAAAAGTATTAA
ACTCCGCAGAAGAAAGTGACAAACGTAGAAAAGAAGGAAAACCACTTTCGGAATTCGACGGAATGCCGGTTGCAATCAAA
GATAATATATGTATTCGGGATACGATCACTTCTTGTTCTTCTAAAATATTAGAAAATTATAAATCTCCATTTCACGCAAC
TGTTGTAGAAAAACTTTTAGAAAAAGGTTTTATACTATTTCCAAGAACTAATATGGACGAGTTTGCGATGGGAAGCTCCA
CGGAAAATTCCGCCTTTCAGACAACTCGAAATCCTTTTGACCTGGAAAGAATTCCGGGTGGTTCCAGCGGAGGTTCTGCA
GCGGCAGTTGCCGCTTCTATGGTTCCACTGGCACTTGGATCGGATACGGGAGGATCGGTTCGTCAACCTGCTTCTCTTTG
TGGGCTCTACGGACTCAAACCTACTTATGGAACCGTTTCCCGTTATGGGCTTGTAGCTTATGCTTCTAGTTTAGATCAGA
TCGGACCATTTTCTAGAGAACTACAAGGTTGTATAGATTTGTATTCTGTAATTTCCGGAAAAGACGTAAGGGATTCTACT
TCTATCCATCGTCCCAAATTTTCGGCTTCGGATGTTCAGCCCCAGGATTTTAAAGGATTGAAAGTAGGGGTCATTAAAAT
GACTCCAGAAATTCAATCTGAAGTAGTAAAATCGTATGATAAAGTTCTAAATCAGTTAAAAGAAAAAGGCGCTACGTTAG
TTGATATTGATTTTTCGAAATTCGGTTTTGCAATTCCAATCTATTATATCATAGCGACTGCGGAATGTTCTTCCAACCTT
TCCCGGTTTGACGGGATTCGTTTTGGATCTAGAAAAGATAAAACTGGAAAACTAGAAGATCTTTTTGTAGATTCTAGAAC
GGAAGGATTTGGACCGGAAGTAAAGAGAAGAATTCTTTTGGGAACTTTTTCTTTGTCTGCAGGTTATTATGACGCTTATT
ATGGAACCGCACAAAAGGCAAGGGCTTTGATTCGTAAGGAATATGAATCTTTCTTTTCAAAAGTAGATTGTATATTACAA
CCTACTTCTCCTACGACTGCGTTTAAGATTGGTGAAAAAACGAAAGATCCAATTCAGATGTATAAAGCTGATATTTGGAC
TACTAGCGTAAATCTCGCCGGACTTCCTGCAATGAGTGTTCCTATGGGAGCGGATCAAAAAGGGCTTCCAATCGGATTAC
AAATTACTACTCCTCATTTTCAAGAAGGAAAACTTTTCGGAATTGCATTAGCACTTTCTACATTAGAAGGAATGAATATT
CAGTTTCCGGAAAGTATTAAATGA

Upstream 100 bases:

>100_bases
GTCCTGATTTGGCTGGTAAACATTTGTCACGAGAAGAGATAGAGTCATTTGCACCTTCTTTTCAAAATGGATATTTCGTG
GTTCCTAAGGTGATAGAAAC

Downstream 100 bases:

>100_bases
GTAATCTTACTGCCAGAGTAATTCCCTGTCTGGACATCAAAGACGGGCGTGTAGTTAAGGGAGTAAATTTTGTAAATCTA
GTGGACGCTGGAGATCCAGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MNEILKKPYVELKDSLNSGKISSTELVSACINRIREVDGAVKAFLYLDEKKVLNSAEESDKRRKEGKPLSEFDGMPVAIK
DNICIRDTITSCSSKILENYKSPFHATVVEKLLEKGFILFPRTNMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSA
AAVAASMVPLALGSDTGGSVRQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSRELQGCIDLYSVISGKDVRDST
SIHRPKFSASDVQPQDFKGLKVGVIKMTPEIQSEVVKSYDKVLNQLKEKGATLVDIDFSKFGFAIPIYYIIATAECSSNL
SRFDGIRFGSRKDKTGKLEDLFVDSRTEGFGPEVKRRILLGTFSLSAGYYDAYYGTAQKARALIRKEYESFFSKVDCILQ
PTSPTTAFKIGEKTKDPIQMYKADIWTTSVNLAGLPAMSVPMGADQKGLPIGLQITTPHFQEGKLFGIALALSTLEGMNI
QFPESIK

Sequences:

>Translated_487_residues
MNEILKKPYVELKDSLNSGKISSTELVSACINRIREVDGAVKAFLYLDEKKVLNSAEESDKRRKEGKPLSEFDGMPVAIK
DNICIRDTITSCSSKILENYKSPFHATVVEKLLEKGFILFPRTNMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSA
AAVAASMVPLALGSDTGGSVRQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSRELQGCIDLYSVISGKDVRDST
SIHRPKFSASDVQPQDFKGLKVGVIKMTPEIQSEVVKSYDKVLNQLKEKGATLVDIDFSKFGFAIPIYYIIATAECSSNL
SRFDGIRFGSRKDKTGKLEDLFVDSRTEGFGPEVKRRILLGTFSLSAGYYDAYYGTAQKARALIRKEYESFFSKVDCILQ
PTSPTTAFKIGEKTKDPIQMYKADIWTTSVNLAGLPAMSVPMGADQKGLPIGLQITTPHFQEGKLFGIALALSTLEGMNI
QFPESIK
>Mature_487_residues
MNEILKKPYVELKDSLNSGKISSTELVSACINRIREVDGAVKAFLYLDEKKVLNSAEESDKRRKEGKPLSEFDGMPVAIK
DNICIRDTITSCSSKILENYKSPFHATVVEKLLEKGFILFPRTNMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSA
AAVAASMVPLALGSDTGGSVRQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSRELQGCIDLYSVISGKDVRDST
SIHRPKFSASDVQPQDFKGLKVGVIKMTPEIQSEVVKSYDKVLNQLKEKGATLVDIDFSKFGFAIPIYYIIATAECSSNL
SRFDGIRFGSRKDKTGKLEDLFVDSRTEGFGPEVKRRILLGTFSLSAGYYDAYYGTAQKARALIRKEYESFFSKVDCILQ
PTSPTTAFKIGEKTKDPIQMYKADIWTTSVNLAGLPAMSVPMGADQKGLPIGLQITTPHFQEGKLFGIALALSTLEGMNI
QFPESIK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=507, Percent_Identity=34.9112426035503, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI195972892, Length=478, Percent_Identity=23.0125523012552, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=412, Percent_Identity=35.6796116504854, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17537465, Length=490, Percent_Identity=24.4897959183673, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=327, Percent_Identity=27.217125382263, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=30.2083333333333, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=196, Percent_Identity=32.6530612244898, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=196, Percent_Identity=32.6530612244898, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=299, Percent_Identity=25.4180602006689, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=308, Percent_Identity=25.6493506493506, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=410, Percent_Identity=36.0975609756098, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=30.188679245283, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=522, Percent_Identity=23.5632183908046, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648113, Length=447, Percent_Identity=37.1364653243848, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=26.5748031496063, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652985, Length=487, Percent_Identity=24.6406570841889, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922090, Length=487, Percent_Identity=24.6406570841889, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652981, Length=487, Percent_Identity=24.6406570841889, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652983, Length=487, Percent_Identity=24.6406570841889, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356731, Length=480, Percent_Identity=25, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550774, Length=213, Percent_Identity=34.2723004694836, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648435, Length=213, Percent_Identity=34.2723004694836, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648437, Length=213, Percent_Identity=34.2723004694836, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078093, Length=494, Percent_Identity=24.8987854251012, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=38.1294964028777, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=38.1294964028777, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_LEPIC (Q72SC3)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001422.1
- ProteinModelPortal:   Q72SC3
- SMR:   Q72SC3
- GeneID:   2771369
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11461
- NMPDR:   fig|267671.1.peg.1422
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   LINT267671:LIC_11461-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53287; Mature: 53287

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEILKKPYVELKDSLNSGKISSTELVSACINRIREVDGAVKAFLYLDEKKVLNSAEESD
CCHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
KRRKEGKPLSEFDGMPVAIKDNICIRDTITSCSSKILENYKSPFHATVVEKLLEKGFILF
HHHHCCCCHHHHCCCCEEEECCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
PRTNMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSAAAVAASMVPLALGSDTGGSV
ECCCCHHHHCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCCC
RQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSRELQGCIDLYSVISGKDVRDST
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
SIHRPKFSASDVQPQDFKGLKVGVIKMTPEIQSEVVKSYDKVLNQLKEKGATLVDIDFSK
CCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHH
FGFAIPIYYIIATAECSSNLSRFDGIRFGSRKDKTGKLEDLFVDSRTEGFGPEVKRRILL
HCHHEEEEHEEEEHHHHCCHHHHCCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHHEEEE
GTFSLSAGYYDAYYGTAQKARALIRKEYESFFSKVDCILQPTSPTTAFKIGEKTKDPIQM
EEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCCCCCCHHHH
YKADIWTTSVNLAGLPAMSVPMGADQKGLPIGLQITTPHFQEGKLFGIALALSTLEGMNI
HHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHCCCCC
QFPESIK
CCCCCCC
>Mature Secondary Structure
MNEILKKPYVELKDSLNSGKISSTELVSACINRIREVDGAVKAFLYLDEKKVLNSAEESD
CCHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
KRRKEGKPLSEFDGMPVAIKDNICIRDTITSCSSKILENYKSPFHATVVEKLLEKGFILF
HHHHCCCCHHHHCCCCEEEECCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
PRTNMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSAAAVAASMVPLALGSDTGGSV
ECCCCHHHHCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCCC
RQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSRELQGCIDLYSVISGKDVRDST
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
SIHRPKFSASDVQPQDFKGLKVGVIKMTPEIQSEVVKSYDKVLNQLKEKGATLVDIDFSK
CCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHH
FGFAIPIYYIIATAECSSNLSRFDGIRFGSRKDKTGKLEDLFVDSRTEGFGPEVKRRILL
HCHHEEEEHEEEEHHHHCCHHHHCCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHHEEEE
GTFSLSAGYYDAYYGTAQKARALIRKEYESFFSKVDCILQPTSPTTAFKIGEKTKDPIQM
EEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCCCCCCHHHH
YKADIWTTSVNLAGLPAMSVPMGADQKGLPIGLQITTPHFQEGKLFGIALALSTLEGMNI
HHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHCCCCC
QFPESIK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA