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The map label for this gene is algI [H]

Identifier: 45657131

GI number: 45657131

Start: 1541817

End: 1543241

Strand: Direct

Name: algI [H]

Synonym: LIC11248

Alternate gene names: 45657131

Gene position: 1541817-1543241 (Clockwise)

Preceding gene: 45657130

Following gene: 45657132

Centisome position: 36.05

GC content: 33.4

Gene sequence:

>1425_bases
ATGTTATTCAATTCTCTGAATTTTCTCCTTTTTTTTATAAGTTTTTGTTTCATCTATTTTCGCCTCGGTTTGAAAGGACA
GAACTATTTTTTGTTTTTTGGAGGTTTGTTTTTTTACGGTTATTGGAAACCGGAGATGGTTTTACTTTTGTTCTTTTGTA
TTTCTTTTAATTTTGCAGGGGGAATTTGGCTTGGTAAAACGTCTGCGGGAATTTACGGAAAACGAATCTTTGTTTTTTTA
ATTGTTTTGAATCTGGGAATTCTGATCTTTTTTAAGTATATTCTTTTTTTCTTAAGTGTTTGGAATGATTCTCTGGGAGT
TTTGTTTCCATTTTCGATTCTTCCCATTCCAGAAATTTTATTGCCGATAGGAATTTCCTTTTATACGTTTCACAATATCA
GTTATCTTTCGGATATTCGATCCGGAAAAATAGTTCCTTGTTCCAATTTTATTCGTTTTGGAGTTTATGATTTATTTTTT
CCATTGTTGTTAGCTGGACCTATCGAAAGACCGGATTCCTTATTACCTCAGATCGAAAAGGAACGAGTAGTAGATCAAAA
CGATTTTGTTTCCGGAGCGATTCTATTTTTTTGGGGAATATTTAAGAAGGTGTTTGTAGGAGATCATCTTTTATTTTTTA
CCAATAAGGTAATGGAACCTGGAATGTCTCTTCCCTCTGGTATGATATTTTGGATCGCATTTTCTTTTGCGTTTCAGGTT
TATGCCGACTTTAGCGGATACACGGATGGGGCACGTGGGCTTGCAAAGATGTTAGGTTTTAAACTTTCCTTAAACTTTAA
TTTTCCTTTTATCTCGTCTAACCCTTCCGAATTTTGGAAACGATGGCATATCTCTCTTTCTACTTGGTTAAGGGATTATC
TTTATATTCCTTTGGGTGGAAATAGGATTTCTTTGTTTCGTCAAAATGTAAATTTGATGATCGTTTGGATTTTAGGGGGA
CTTTGGCACGGGGCTACATACGGGTATTTGGTATGGGGATTTTATTGTGGTTTGCAGGTTGTGCTCTATAATATAGTTCA
AAAATATTTTTTAAATTTAATTAATAATAATATTAAAATTTTAAAATATTCTATAAAGCTAATTGGAGTTTTTTTGACGT
TCTGGATGTTTGCTTTTGGATTACTTTTGTTTCAGGTGCATTCTCCAGGAGAACTTTGGAGTTTAGCCGGAAACGCATTT
GGAGGATTTTACTGGAATTCCGTGTTTGCGGCTAAATTGTTTTTTCTTTGTTTTCCTTTGTTTTGTGTGGAATTTTGGAT
GATTGTTTGGGGAGGAACCGATTTATTTTTAGAGAAGATCGTTGCAACACGATATAGCTGGATTTTGTTTTCTTTTGGGA
TTGGTATTTTATTTTGCCTCTTTGGAGTTTTTGAAAAAAAAGAGTTCTTTTACTTTCAGTTTTAA

Upstream 100 bases:

>100_bases
TTCAGACTTCTGTTGTGCAACATACTCTTTGTTATCCTGCGGATACAAAAGAAAAAGAAAGAATTTTAGAATTACTCGAA
GAGTCTTAGATCGATAGAGA

Downstream 100 bases:

>100_bases
GATGGTTCCCAAGCTTAAGATTTTGTCGAGTTGTTAACAAAATGTAGTAGTTCACACGGATATTTAAGGAAGGTTGGGTA
ATACATAGTTGTATTTATGA

Product: alginate o-acetyltransferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLFNSLNFLLFFISFCFIYFRLGLKGQNYFLFFGGLFFYGYWKPEMVLLLFFCISFNFAGGIWLGKTSAGIYGKRIFVFL
IVLNLGILIFFKYILFFLSVWNDSLGVLFPFSILPIPEILLPIGISFYTFHNISYLSDIRSGKIVPCSNFIRFGVYDLFF
PLLLAGPIERPDSLLPQIEKERVVDQNDFVSGAILFFWGIFKKVFVGDHLLFFTNKVMEPGMSLPSGMIFWIAFSFAFQV
YADFSGYTDGARGLAKMLGFKLSLNFNFPFISSNPSEFWKRWHISLSTWLRDYLYIPLGGNRISLFRQNVNLMIVWILGG
LWHGATYGYLVWGFYCGLQVVLYNIVQKYFLNLINNNIKILKYSIKLIGVFLTFWMFAFGLLLFQVHSPGELWSLAGNAF
GGFYWNSVFAAKLFFLCFPLFCVEFWMIVWGGTDLFLEKIVATRYSWILFSFGIGILFCLFGVFEKKEFFYFQF

Sequences:

>Translated_474_residues
MLFNSLNFLLFFISFCFIYFRLGLKGQNYFLFFGGLFFYGYWKPEMVLLLFFCISFNFAGGIWLGKTSAGIYGKRIFVFL
IVLNLGILIFFKYILFFLSVWNDSLGVLFPFSILPIPEILLPIGISFYTFHNISYLSDIRSGKIVPCSNFIRFGVYDLFF
PLLLAGPIERPDSLLPQIEKERVVDQNDFVSGAILFFWGIFKKVFVGDHLLFFTNKVMEPGMSLPSGMIFWIAFSFAFQV
YADFSGYTDGARGLAKMLGFKLSLNFNFPFISSNPSEFWKRWHISLSTWLRDYLYIPLGGNRISLFRQNVNLMIVWILGG
LWHGATYGYLVWGFYCGLQVVLYNIVQKYFLNLINNNIKILKYSIKLIGVFLTFWMFAFGLLLFQVHSPGELWSLAGNAF
GGFYWNSVFAAKLFFLCFPLFCVEFWMIVWGGTDLFLEKIVATRYSWILFSFGIGILFCLFGVFEKKEFFYFQF
>Mature_474_residues
MLFNSLNFLLFFISFCFIYFRLGLKGQNYFLFFGGLFFYGYWKPEMVLLLFFCISFNFAGGIWLGKTSAGIYGKRIFVFL
IVLNLGILIFFKYILFFLSVWNDSLGVLFPFSILPIPEILLPIGISFYTFHNISYLSDIRSGKIVPCSNFIRFGVYDLFF
PLLLAGPIERPDSLLPQIEKERVVDQNDFVSGAILFFWGIFKKVFVGDHLLFFTNKVMEPGMSLPSGMIFWIAFSFAFQV
YADFSGYTDGARGLAKMLGFKLSLNFNFPFISSNPSEFWKRWHISLSTWLRDYLYIPLGGNRISLFRQNVNLMIVWILGG
LWHGATYGYLVWGFYCGLQVVLYNIVQKYFLNLINNNIKILKYSIKLIGVFLTFWMFAFGLLLFQVHSPGELWSLAGNAF
GGFYWNSVFAAKLFFLCFPLFCVEFWMIVWGGTDLFLEKIVATRYSWILFSFGIGILFCLFGVFEKKEFFYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 55184; Mature: 55184

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSLNFLLFFISFCFIYFRLGLKGQNYFLFFGGLFFYGYWKPEMVLLLFFCISFNFAG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHCCCC
GIWLGKTSAGIYGKRIFVFLIVLNLGILIFFKYILFFLSVWNDSLGVLFPFSILPIPEIL
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHCCHHHHH
LPIGISFYTFHNISYLSDIRSGKIVPCSNFIRFGVYDLFFPLLLAGPIERPDSLLPQIEK
HHCCCHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHH
ERVVDQNDFVSGAILFFWGIFKKVFVGDHLLFFTNKVMEPGMSLPSGMIFWIAFSFAFQV
HHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YADFSGYTDGARGLAKMLGFKLSLNFNFPFISSNPSEFWKRWHISLSTWLRDYLYIPLGG
HHCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCC
NRISLFRQNVNLMIVWILGGLWHGATYGYLVWGFYCGLQVVLYNIVQKYFLNLINNNIKI
CEEEEEECCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LKYSIKLIGVFLTFWMFAFGLLLFQVHSPGELWSLAGNAFGGFYWNSVFAAKLFFLCFPL
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FCVEFWMIVWGGTDLFLEKIVATRYSWILFSFGIGILFCLFGVFEKKEFFYFQF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLFNSLNFLLFFISFCFIYFRLGLKGQNYFLFFGGLFFYGYWKPEMVLLLFFCISFNFAG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHCCCC
GIWLGKTSAGIYGKRIFVFLIVLNLGILIFFKYILFFLSVWNDSLGVLFPFSILPIPEIL
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHCCHHHHH
LPIGISFYTFHNISYLSDIRSGKIVPCSNFIRFGVYDLFFPLLLAGPIERPDSLLPQIEK
HHCCCHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHH
ERVVDQNDFVSGAILFFWGIFKKVFVGDHLLFFTNKVMEPGMSLPSGMIFWIAFSFAFQV
HHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YADFSGYTDGARGLAKMLGFKLSLNFNFPFISSNPSEFWKRWHISLSTWLRDYLYIPLGG
HHCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCC
NRISLFRQNVNLMIVWILGGLWHGATYGYLVWGFYCGLQVVLYNIVQKYFLNLINNNIKI
CEEEEEECCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LKYSIKLIGVFLTFWMFAFGLLLFQVHSPGELWSLAGNAFGGFYWNSVFAAKLFFLCFPL
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FCVEFWMIVWGGTDLFLEKIVATRYSWILFSFGIGILFCLFGVFEKKEFFYFQF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]