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The map label for this gene is vatB

Identifier: 45656439

GI number: 45656439

Start: 660943

End: 661599

Strand: Reverse

Name: vatB

Synonym: LIC10541

Alternate gene names: 45656439

Gene position: 661599-660943 (Counterclockwise)

Preceding gene: 45656441

Following gene: 45656438

Centisome position: 15.47

GC content: 36.83

Gene sequence:

>657_bases
ATGCAAAATTCAGACTCGCATTTTTTCGGACCGAACCCTTCTACTCCTTATCCTTTTCCGGAATTTCCTAGAATCGGTTT
TTTAAAAAACTTTATTCAGTCTACTTTGATTGAAGTAGGAGATTATACCTATTACGATGATCCGGATGGTCCGGAACATT
TTGAAAAGAAAAATGTTCTATATCACTACGATTTTCGAGGAGATAAACTTTTAATCGGAAAATTTTGCGCGATTGCAACT
GGTGTAAAATTCATCATGAATGGAGCCAATCATAAGACGAGTGCATTCTCTACATTTCCTTTTGCGATTTTTTCAAACGG
ATGGGAAAAATCCATGCCTAAACCTGGAGATTTTCCGGATAAAGGAGATACGATTGTAGGTAACGACGTTTGGATTGGAA
CCAATGCAATCATTCTCCCAGGAATTAAAATTGGAGATGGGGCAATTATAGGTGCTTATTCGGTTGTAGCCAGAGATGTT
CCGGCTTATACGATCGTTGCCGGAAATCCTTCTCAAGTGATTCGAGAAAGATTTTCTCCAGAAGTCAAAGAAAAACTTTT
AAAATTGAAGTGGTGGGATTGGACTCCTCAAAAGATTACGGATTCATTAGAATTTTTGACAACTCTTGATTTTAAAAAAC
TAGATGAATTTGATTAG

Upstream 100 bases:

>100_bases
GTCTTAAATTTTGAATAACTCTTGTAAAAACCGAATTCTATAGTCGGTTGCAAGGTAAAAGAGATATGTTTTTAAGACAA
TCATTAAGGAGATTGTATCG

Downstream 100 bases:

>100_bases
ACTCGGATGTTGTAAATTCGATTCAAATCATTCGGAAAACTGAGAAACAGAATACAAGAATAAGGAAAGCAGTTTAAGCC
ATCCATAAACCCTTTTTTAA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MQNSDSHFFGPNPSTPYPFPEFPRIGFLKNFIQSTLIEVGDYTYYDDPDGPEHFEKKNVLYHYDFRGDKLLIGKFCAIAT
GVKFIMNGANHKTSAFSTFPFAIFSNGWEKSMPKPGDFPDKGDTIVGNDVWIGTNAIILPGIKIGDGAIIGAYSVVARDV
PAYTIVAGNPSQVIRERFSPEVKEKLLKLKWWDWTPQKITDSLEFLTTLDFKKLDEFD

Sequences:

>Translated_218_residues
MQNSDSHFFGPNPSTPYPFPEFPRIGFLKNFIQSTLIEVGDYTYYDDPDGPEHFEKKNVLYHYDFRGDKLLIGKFCAIAT
GVKFIMNGANHKTSAFSTFPFAIFSNGWEKSMPKPGDFPDKGDTIVGNDVWIGTNAIILPGIKIGDGAIIGAYSVVARDV
PAYTIVAGNPSQVIRERFSPEVKEKLLKLKWWDWTPQKITDSLEFLTTLDFKKLDEFD
>Mature_218_residues
MQNSDSHFFGPNPSTPYPFPEFPRIGFLKNFIQSTLIEVGDYTYYDDPDGPEHFEKKNVLYHYDFRGDKLLIGKFCAIAT
GVKFIMNGANHKTSAFSTFPFAIFSNGWEKSMPKPGDFPDKGDTIVGNDVWIGTNAIILPGIKIGDGAIIGAYSVVARDV
PAYTIVAGNPSQVIRERFSPEVKEKLLKLKWWDWTPQKITDSLEFLTTLDFKKLDEFD

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=51, Percent_Identity=50.9803921568627, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 24618; Mature: 24618

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSDSHFFGPNPSTPYPFPEFPRIGFLKNFIQSTLIEVGDYTYYDDPDGPEHFEKKNVL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEE
YHYDFRGDKLLIGKFCAIATGVKFIMNGANHKTSAFSTFPFAIFSNGWEKSMPKPGDFPD
EEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCC
KGDTIVGNDVWIGTNAIILPGIKIGDGAIIGAYSVVARDVPAYTIVAGNPSQVIRERFSP
CCCEEECCCEEECCCEEEECCEEECCCEEEHHHHHHHCCCCEEEEEECCHHHHHHHHCCH
EVKEKLLKLKWWDWTPQKITDSLEFLTTLDFKKLDEFD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHCCCC
>Mature Secondary Structure
MQNSDSHFFGPNPSTPYPFPEFPRIGFLKNFIQSTLIEVGDYTYYDDPDGPEHFEKKNVL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEE
YHYDFRGDKLLIGKFCAIATGVKFIMNGANHKTSAFSTFPFAIFSNGWEKSMPKPGDFPD
EEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCC
KGDTIVGNDVWIGTNAIILPGIKIGDGAIIGAYSVVARDVPAYTIVAGNPSQVIRERFSP
CCCEEECCCEEECCCEEEECCEEECCCEEEHHHHHHHCCCCEEEEEECCHHHHHHHHCCH
EVKEKLLKLKWWDWTPQKITDSLEFLTTLDFKKLDEFD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]