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The map label for this gene is fadE25 [H]

Identifier: 45656441

GI number: 45656441

Start: 663201

End: 664916

Strand: Reverse

Name: fadE25 [H]

Synonym: LIC10543

Alternate gene names: 45656441

Gene position: 664916-663201 (Counterclockwise)

Preceding gene: 45656446

Following gene: 45656439

Centisome position: 15.55

GC content: 37.35

Gene sequence:

>1716_bases
ATGATTCAAAATAATTATTTTTCGGACAATCAAGACATCCAGGATCATTTTGTACACATTCTACCTTGGACAGAAATTAT
ACTCGATTACGAAAACGATTTTTCTGGTGTTTCAGAAGGAGGACCTTCGAACCCTTCTGAAGCAAAAGAATATTATAAAA
CTGTGTTAGAAACGGTCGGAGATTTAGCAGGAAATATACTTTCTCCGCACGTAGCCGATCTGGATCGAGAGGGTTTAAAA
TTTAAAAACGGAAAAGTAGAATTCCCTCCCAAAATGCTCGAATTAGTTTCTAAGGTTATAGAAGCAGGAGTTCAAGCCTA
CGGATTTTCTAGAAAATACGGAGGATTAGGAATTCCTTGGACAGTTAAATCCTTTATATCAGAAATTTTTTATAGAGTCG
ACGCGTCACTCGCCATTGCCATCGGATGTGTAAATCTTGCGGAAATTTTAGAACGTCATGCAACCGAGCAAATGAAGAGC
GACTGGATACCTCATTTTGCCGATGGAAAGTTTGTTTGTGCGATGGGGCTCACTGAACCAGATCACGGCTCCGATCTTTC
TAATTTAAGAACCAAAGCCACAAAAGATAAAAACGGAAATTGGACATTAAACGGAACCAAACGTTTTATTACACATGGTT
GTGGATTTGGAGATCTCCCTGCGGTTTTACTGACACTAGCTCGTTCGGGTGAGATAAATTCCGGTGCGAGAGGACTTTCT
TTTTTTCTAGTTCAAAGTACGGATGTTCAAATTGCAGGAATCGAAAATAAACTTGGACTTCATTGTTCTCCCACCTGTGA
GGTAGTTTTTGAAAATTCTCCCGGACTTTTAATCGGTGAAGAAGGTTACGGACTTATAAAATATACGATGGGAATGTTAA
ACGGAGCCAGAATGGGAATTGCACAACAATCCACTGGCCTTGCAACTGCAGCATATTATGAAGCTCTAAAATATGCAAAA
GAAAGAACCCAATTCGGAAAAACTTTGATAGAAATCCCAGCGGTAAAAAAGATATTGGATCAGATCGAAAGAGAAACATA
CGCTATGAGATGTCTGACTCTGGAAGGATCTAGAGTTATGGATCGTTATTATTGGAGAGCAATTCGACTTGAAAAACAAG
GAGCTACAGAAAAAGAAATTAAAAACGATACAGTGGTTCGTTATTGGGAGAAAATCGCAAACATTCTTACACCAATTAGT
AAGTTTTATTGTTCCGAATCTTGTTTAAAGACGGTCAGTGACGCCCTTCAGGTTCACGGTGGTTCTGGTTATACGGAAGA
TTATGATATTTCTAGAATTTATAGAGACGCAAGGATTGTTACGATCTATGACGGAACTTCTCAGATTCAAATCAATGCTT
GTATCGGAGGAATTACTTCCGGCTTAACTCATACGTTTGGAGAATACGTATCCGAATTGATAAGCCGATCCGACTCTTCT
TTTACTCATAAACTGTTTTACGGATTTCAAGAATTAGTAAAACTTTATAAGGAACTTCCGGAAAAAGAAATGAAAGATAT
ATATGCGGAAGAGATCGTATTGACTTGTTCCAGACTTTTAGCGGGAATTTTATTTGAACTTTCTTGTAAAAGACTTCCGG
AAGATAAAAAATTAGTTCGGCTCAAACACGTAAAAGATTATCATATAGATACTCTTTCTGTTTTGGAAGGAAATCTCGCA
AAATTAAAAGAAGTAAACAAAATTAAAGTTTTATAA

Upstream 100 bases:

>100_bases
TTGTTTTAAAAAAGGTTTAAACTTCTATTCCTTTTAAGTTCGTTTTTTACTTCTCTTTGTTTTCTGTTCTTTTATACTGA
CTGATTCAAGGGAGAATACA

Downstream 100 bases:

>100_bases
TTTGTTTAAAAAGCTTTCTAGAAAATTTTTAAAAAATCAACTTTCTTTTTTATTAAAGAATTTGTTTCAAAACCTCAAAG
AATTTTAAGCGATAATTCTT

Product: acyl-CoA dehydrogenase

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MIQNNYFSDNQDIQDHFVHILPWTEIILDYENDFSGVSEGGPSNPSEAKEYYKTVLETVGDLAGNILSPHVADLDREGLK
FKNGKVEFPPKMLELVSKVIEAGVQAYGFSRKYGGLGIPWTVKSFISEIFYRVDASLAIAIGCVNLAEILERHATEQMKS
DWIPHFADGKFVCAMGLTEPDHGSDLSNLRTKATKDKNGNWTLNGTKRFITHGCGFGDLPAVLLTLARSGEINSGARGLS
FFLVQSTDVQIAGIENKLGLHCSPTCEVVFENSPGLLIGEEGYGLIKYTMGMLNGARMGIAQQSTGLATAAYYEALKYAK
ERTQFGKTLIEIPAVKKILDQIERETYAMRCLTLEGSRVMDRYYWRAIRLEKQGATEKEIKNDTVVRYWEKIANILTPIS
KFYCSESCLKTVSDALQVHGGSGYTEDYDISRIYRDARIVTIYDGTSQIQINACIGGITSGLTHTFGEYVSELISRSDSS
FTHKLFYGFQELVKLYKELPEKEMKDIYAEEIVLTCSRLLAGILFELSCKRLPEDKKLVRLKHVKDYHIDTLSVLEGNLA
KLKEVNKIKVL

Sequences:

>Translated_571_residues
MIQNNYFSDNQDIQDHFVHILPWTEIILDYENDFSGVSEGGPSNPSEAKEYYKTVLETVGDLAGNILSPHVADLDREGLK
FKNGKVEFPPKMLELVSKVIEAGVQAYGFSRKYGGLGIPWTVKSFISEIFYRVDASLAIAIGCVNLAEILERHATEQMKS
DWIPHFADGKFVCAMGLTEPDHGSDLSNLRTKATKDKNGNWTLNGTKRFITHGCGFGDLPAVLLTLARSGEINSGARGLS
FFLVQSTDVQIAGIENKLGLHCSPTCEVVFENSPGLLIGEEGYGLIKYTMGMLNGARMGIAQQSTGLATAAYYEALKYAK
ERTQFGKTLIEIPAVKKILDQIERETYAMRCLTLEGSRVMDRYYWRAIRLEKQGATEKEIKNDTVVRYWEKIANILTPIS
KFYCSESCLKTVSDALQVHGGSGYTEDYDISRIYRDARIVTIYDGTSQIQINACIGGITSGLTHTFGEYVSELISRSDSS
FTHKLFYGFQELVKLYKELPEKEMKDIYAEEIVLTCSRLLAGILFELSCKRLPEDKKLVRLKHVKDYHIDTLSVLEGNLA
KLKEVNKIKVL
>Mature_571_residues
MIQNNYFSDNQDIQDHFVHILPWTEIILDYENDFSGVSEGGPSNPSEAKEYYKTVLETVGDLAGNILSPHVADLDREGLK
FKNGKVEFPPKMLELVSKVIEAGVQAYGFSRKYGGLGIPWTVKSFISEIFYRVDASLAIAIGCVNLAEILERHATEQMKS
DWIPHFADGKFVCAMGLTEPDHGSDLSNLRTKATKDKNGNWTLNGTKRFITHGCGFGDLPAVLLTLARSGEINSGARGLS
FFLVQSTDVQIAGIENKLGLHCSPTCEVVFENSPGLLIGEEGYGLIKYTMGMLNGARMGIAQQSTGLATAAYYEALKYAK
ERTQFGKTLIEIPAVKKILDQIERETYAMRCLTLEGSRVMDRYYWRAIRLEKQGATEKEIKNDTVVRYWEKIANILTPIS
KFYCSESCLKTVSDALQVHGGSGYTEDYDISRIYRDARIVTIYDGTSQIQINACIGGITSGLTHTFGEYVSELISRSDSS
FTHKLFYGFQELVKLYKELPEKEMKDIYAEEIVLTCSRLLAGILFELSCKRLPEDKKLVRLKHVKDYHIDTLSVLEGNLA
KLKEVNKIKVL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=403, Percent_Identity=30.0248138957816, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI21361497, Length=369, Percent_Identity=30.6233062330623, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4557233, Length=413, Percent_Identity=30.0242130750605, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4557231, Length=423, Percent_Identity=28.8416075650118, Blast_Score=124, Evalue=4e-28,
Organism=Homo sapiens, GI187960098, Length=423, Percent_Identity=28.8416075650118, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI4501857, Length=307, Percent_Identity=29.9674267100977, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI4557235, Length=404, Percent_Identity=27.4752475247525, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI7656849, Length=409, Percent_Identity=26.6503667481663, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI76496475, Length=404, Percent_Identity=27.4752475247525, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI226958412, Length=416, Percent_Identity=24.5192307692308, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI226958414, Length=354, Percent_Identity=24.2937853107345, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI4503943, Length=373, Percent_Identity=24.9329758713137, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI7669494, Length=364, Percent_Identity=25.2747252747253, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI87081958, Length=363, Percent_Identity=25.3443526170799, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082384, Length=304, Percent_Identity=26.3157894736842, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786223, Length=394, Percent_Identity=22.3350253807107, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508101, Length=418, Percent_Identity=26.0765550239234, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17533517, Length=401, Percent_Identity=29.1770573566085, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17506239, Length=428, Percent_Identity=25.9345794392523, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71985184, Length=344, Percent_Identity=28.7790697674419, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17534899, Length=397, Percent_Identity=28.463476070529, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17570075, Length=373, Percent_Identity=28.686327077748, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538396, Length=404, Percent_Identity=25.7425742574257, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17569725, Length=412, Percent_Identity=25.9708737864078, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71990804, Length=272, Percent_Identity=27.5735294117647, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534353, Length=306, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI86563383, Length=375, Percent_Identity=25.3333333333333, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI86563381, Length=375, Percent_Identity=25.3333333333333, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17551932, Length=391, Percent_Identity=25.5754475703325, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985192, Length=246, Percent_Identity=30.8943089430894, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505929, Length=409, Percent_Identity=23.2273838630807, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32563615, Length=234, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982178, Length=289, Percent_Identity=25.9515570934256, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356377, Length=414, Percent_Identity=30.1932367149758, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24666513, Length=413, Percent_Identity=27.3607748184019, Blast_Score=142, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24646207, Length=409, Percent_Identity=29.0953545232274, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281363737, Length=351, Percent_Identity=27.9202279202279, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24660351, Length=400, Percent_Identity=27.75, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21355753, Length=400, Percent_Identity=26.25, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920834, Length=280, Percent_Identity=28.9285714285714, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 63912; Mature: 63912

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNNYFSDNQDIQDHFVHILPWTEIILDYENDFSGVSEGGPSNPSEAKEYYKTVLETVG
CCCCCCCCCCCCHHHHHEEHCCHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DLAGNILSPHVADLDREGLKFKNGKVEFPPKMLELVSKVIEAGVQAYGFSRKYGGLGIPW
HHHHHHCCCHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
TVKSFISEIFYRVDASLAIAIGCVNLAEILERHATEQMKSDWIPHFADGKFVCAMGLTEP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
DHGSDLSNLRTKATKDKNGNWTLNGTKRFITHGCGFGDLPAVLLTLARSGEINSGARGLS
CCCCCHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEE
FFLVQSTDVQIAGIENKLGLHCSPTCEVVFENSPGLLIGEEGYGLIKYTMGMLNGARMGI
EEEEECCCEEEEECCCCCCCCCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCHHCCC
AQQSTGLATAAYYEALKYAKERTQFGKTLIEIPAVKKILDQIERETYAMRCLTLEGSRVM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHH
DRYYWRAIRLEKQGATEKEIKNDTVVRYWEKIANILTPISKFYCSESCLKTVSDALQVHG
HHHHHHHHEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSGYTEDYDISRIYRDARIVTIYDGTSQIQINACIGGITSGLTHTFGEYVSELISRSDSS
CCCCCCCCHHHHHHCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
FTHKLFYGFQELVKLYKELPEKEMKDIYAEEIVLTCSRLLAGILFELSCKRLPEDKKLVR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LKHVKDYHIDTLSVLEGNLAKLKEVNKIKVL
HHHCCCCCCCHHHHHCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MIQNNYFSDNQDIQDHFVHILPWTEIILDYENDFSGVSEGGPSNPSEAKEYYKTVLETVG
CCCCCCCCCCCCHHHHHEEHCCHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DLAGNILSPHVADLDREGLKFKNGKVEFPPKMLELVSKVIEAGVQAYGFSRKYGGLGIPW
HHHHHHCCCHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
TVKSFISEIFYRVDASLAIAIGCVNLAEILERHATEQMKSDWIPHFADGKFVCAMGLTEP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
DHGSDLSNLRTKATKDKNGNWTLNGTKRFITHGCGFGDLPAVLLTLARSGEINSGARGLS
CCCCCHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEE
FFLVQSTDVQIAGIENKLGLHCSPTCEVVFENSPGLLIGEEGYGLIKYTMGMLNGARMGI
EEEEECCCEEEEECCCCCCCCCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCHHCCC
AQQSTGLATAAYYEALKYAKERTQFGKTLIEIPAVKKILDQIERETYAMRCLTLEGSRVM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHH
DRYYWRAIRLEKQGATEKEIKNDTVVRYWEKIANILTPISKFYCSESCLKTVSDALQVHG
HHHHHHHHEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSGYTEDYDISRIYRDARIVTIYDGTSQIQINACIGGITSGLTHTFGEYVSELISRSDSS
CCCCCCCCHHHHHHCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
FTHKLFYGFQELVKLYKELPEKEMKDIYAEEIVLTCSRLLAGILFELSCKRLPEDKKLVR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LKHVKDYHIDTLSVLEGNLAKLKEVNKIKVL
HHHCCCCCCCHHHHHCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]