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The map label for this gene is glnB

Identifier: 45656339

GI number: 45656339

Start: 502348

End: 502692

Strand: Reverse

Name: glnB

Synonym: LIC10440

Alternate gene names: 45656339

Gene position: 502692-502348 (Counterclockwise)

Preceding gene: 45656340

Following gene: 45656338

Centisome position: 11.75

GC content: 38.26

Gene sequence:

>345_bases
ATGAAATTAATTGTTGCCATCATTCAACCTCATAAATTGGAAGAAGTAAAATCGGAACTTACTAAAAACGAAATTTACAG
ACTTACCGTAAGCGACGTTCAAGGTTACGGTCAGCAAAAGGGAAAAACGGAAGTTTTCAGAGGTCACGAATACCAAGTAA
ACCTTTTGAGAAAGGTTCGTCTCGAAATCGCAGTAAACGACGAATTCGTAAAACCTACGGTAGACGCTATTCTAAAAGCA
GCAAAAACAGGAGATGGTAAAATCGGAGATGGTAAAATTTTTATTACTCCTCTGGAAGATGTAATACGAATTAGAACCGG
AGAAAGAGGAAGTTCCGCGATCTGA

Upstream 100 bases:

>100_bases
AAGAAAAAGAAATTACTGGACTGGATTCGGAAATTCACGGAGAAAAAGGTTATATCCTTTAATTAAATATCTATTAAAGA
ATTTTGAATAAGGAGATTCT

Downstream 100 bases:

>100_bases
AGTAGATGGTGTAAGAGATTTAAGATTGTTTCGTAAAAAGTTGGATTATAAATCTGAATTTTACAGACTTATTTCTAAAA
TGTGAGAACTCTTACTTTTA

Product: nitrogen regulatory protein PII

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MKLIVAIIQPHKLEEVKSELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKTGDGKIGDGKIFITPLEDVIRIRTGERGSSAI

Sequences:

>Translated_114_residues
MKLIVAIIQPHKLEEVKSELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKTGDGKIGDGKIFITPLEDVIRIRTGERGSSAI
>Mature_114_residues
MKLIVAIIQPHKLEEVKSELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKTGDGKIGDGKIFITPLEDVIRIRTGERGSSAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=114, Percent_Identity=50, Blast_Score=112, Evalue=6e-27,
Organism=Escherichia coli, GI1788904, Length=114, Percent_Identity=51.7543859649123, Blast_Score=111, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12762; Mature: 12762

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVAIIQPHKLEEVKSELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVR
CEEEEEEECCCCHHHHHHHHHHHHEEEEEHHHHCCCCCCCCCCCEECCCCHHHHHHHHEE
LEIAVNDEFVKPTVDAILKAAKTGDGKIGDGKIFITPLEDVIRIRTGERGSSAI
EEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MKLIVAIIQPHKLEEVKSELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVR
CEEEEEEECCCCHHHHHHHHHHHHEEEEEHHHHCCCCCCCCCCCEECCCCHHHHHHHHEE
LEIAVNDEFVKPTVDAILKAAKTGDGKIGDGKIFITPLEDVIRIRTGERGSSAI
EEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]