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The map label for this gene is nrgA [H]

Identifier: 45656340

GI number: 45656340

Start: 502731

End: 504023

Strand: Reverse

Name: nrgA [H]

Synonym: LIC10441

Alternate gene names: 45656340

Gene position: 504023-502731 (Counterclockwise)

Preceding gene: 45656341

Following gene: 45656339

Centisome position: 11.78

GC content: 42.07

Gene sequence:

>1293_bases
TTGGGGATAGGAATGATTATGAAATGGACCAAGAGACTGCTCTTATTGCTCACCCTACTTCTTCCATTTGTTTTATTTGG
GCAAGAAGCTAGCACTCCCGTAGCGGACAAAGGAGATACGGTCTGGATGATTGTAGCATCCGCCCTCGTATTTTTTATGA
TACCTGGGCTTGCATTGTTTTATGGAGGTCTTGTAAGATCTAAAAACGTTCTTTCGACTATGATGCATAGTTTTGTAGCA
ATCTTAGTTTTGACCCTGCAGTGGACCATCTTTGGATATAGTTTCGCTTTTTCCGGAACCAATCCTTATTTCGGAAACTT
TGATCTTGCCTTTCTAAATGGAATCGATGAAAACACTTTAGAATTAACAATTCCTAAATATATTCACTTTCTTTTTCAAG
GTATGTTCGCATTAATCACACCCGCCTTAATTTCTGGAGCAATTGCAGAAAGAGTAAAATTCGGCGGTTATATAATTTTT
ATTCTTCTTTGGTCCACTCTTGTTTATGATCCAGTTGCACATTGGGTTTGGGCGGCAGACGGTTGGCTTTTTAAAATGAG
CGCTTTAGATTTTGCTGGTGGAACCGTAGTTCATCTTATCTCAGGAATTGCAGGTCTTGCAGCCGCAATCGTTCTGGGTA
AAAGAAAAGGAGAAGGACCCGCTCTGATCGCTCCCAACAATCTTACTTATACTTTAATCGGAGCCGGTTTACTTTGGTTC
GGTTGGTTCGGTTTTAACGCTGGGTCCGGACTTGCGGTAAACGGAATTACCGCCAGAGCATTTTTAGTTACTTTAATCGC
ACCTGCAGCCGCAGGAGTTGCTTGGCTTGTAATCGAATATTTCCATACTAAAAAAGCAACCGCCCTCGGAGCCGCTTCGG
GTATTGTGGCCGGTTTGGTTGTAATTACTCCCGCTTCTGGTTTTGTAGGAGTTCAAGGAGCAATCATCATGGGACTTCTT
GTAAGTCCGGTATGCTACGCAGCGATTCTTTTAAAAGGAAAACTAGGATACGATGATAGTTTGGACGCATTTGGAATTCA
CGGCGTAGGTGGTGCGTTAGGCGCGGTTCTTACAGGAGTTTTCACTCTTACACTCGGCGCAGGTGTTGCGAGTAAAGGAG
ATCAAATTTTGGTTCAACTCATTAGCGTCGCTGCAACCGGAGCTTATTCTTTTATCGTTTCTATGATTCTAGTATTTTTG
ATCGATAAAACAATCGGCTTTCGTATTTCCGAAGAAAAAGAAATTACTGGACTGGATTCGGAAATTCACGGAGAAAAAGG
TTATATCCTTTAA

Upstream 100 bases:

>100_bases
GTGGGAGCCCACCATTTTAAAGATTACTTTTGGTTTTCTGAAAATTTTTAAATTATTCTTCAATTGGTACTAATCTTGCA
AACAAAGAATAAAAATTGAT

Downstream 100 bases:

>100_bases
TTAAATATCTATTAAAGAATTTTGAATAAGGAGATTCTATGAAATTAATTGTTGCCATCATTCAACCTCATAAATTGGAA
GAAGTAAAATCGGAACTTAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGIGMIMKWTKRLLLLLTLLLPFVLFGQEASTPVADKGDTVWMIVASALVFFMIPGLALFYGGLVRSKNVLSTMMHSFVA
ILVLTLQWTIFGYSFAFSGTNPYFGNFDLAFLNGIDENTLELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIIF
ILLWSTLVYDPVAHWVWAADGWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPALIAPNNLTYTLIGAGLLWF
GWFGFNAGSGLAVNGITARAFLVTLIAPAAAGVAWLVIEYFHTKKATALGAASGIVAGLVVITPASGFVGVQGAIIMGLL
VSPVCYAAILLKGKLGYDDSLDAFGIHGVGGALGAVLTGVFTLTLGAGVASKGDQILVQLISVAATGAYSFIVSMILVFL
IDKTIGFRISEEKEITGLDSEIHGEKGYIL

Sequences:

>Translated_430_residues
MGIGMIMKWTKRLLLLLTLLLPFVLFGQEASTPVADKGDTVWMIVASALVFFMIPGLALFYGGLVRSKNVLSTMMHSFVA
ILVLTLQWTIFGYSFAFSGTNPYFGNFDLAFLNGIDENTLELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIIF
ILLWSTLVYDPVAHWVWAADGWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPALIAPNNLTYTLIGAGLLWF
GWFGFNAGSGLAVNGITARAFLVTLIAPAAAGVAWLVIEYFHTKKATALGAASGIVAGLVVITPASGFVGVQGAIIMGLL
VSPVCYAAILLKGKLGYDDSLDAFGIHGVGGALGAVLTGVFTLTLGAGVASKGDQILVQLISVAATGAYSFIVSMILVFL
IDKTIGFRISEEKEITGLDSEIHGEKGYIL
>Mature_429_residues
GIGMIMKWTKRLLLLLTLLLPFVLFGQEASTPVADKGDTVWMIVASALVFFMIPGLALFYGGLVRSKNVLSTMMHSFVAI
LVLTLQWTIFGYSFAFSGTNPYFGNFDLAFLNGIDENTLELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIIFI
LLWSTLVYDPVAHWVWAADGWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPALIAPNNLTYTLIGAGLLWFG
WFGFNAGSGLAVNGITARAFLVTLIAPAAAGVAWLVIEYFHTKKATALGAASGIVAGLVVITPASGFVGVQGAIIMGLLV
SPVCYAAILLKGKLGYDDSLDAFGIHGVGGALGAVLTGVFTLTLGAGVASKGDQILVQLISVAATGAYSFIVSMILVFLI
DKTIGFRISEEKEITGLDSEIHGEKGYIL

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=406, Percent_Identity=45.5665024630542, Blast_Score=323, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=30.1401869158879, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17550180, Length=421, Percent_Identity=28.7410926365796, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=423, Percent_Identity=27.1867612293144, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=29.0993071593533, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=35.5072463768116, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324187, Length=420, Percent_Identity=38.0952380952381, Blast_Score=256, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=33.9759036144578, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI161078314, Length=406, Percent_Identity=29.5566502463054, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45492; Mature: 45361

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIGMIMKWTKRLLLLLTLLLPFVLFGQEASTPVADKGDTVWMIVASALVFFMIPGLALF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YGGLVRSKNVLSTMMHSFVAILVLTLQWTIFGYSFAFSGTNPYFGNFDLAFLNGIDENTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEECCCCCCEE
ELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIIFILLWSTLVYDPVAHWVWAAD
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
GWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPALIAPNNLTYTLIGAGLLWF
CEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEHHHHHHHHH
GWFGFNAGSGLAVNGITARAFLVTLIAPAAAGVAWLVIEYFHTKKATALGAASGIVAGLV
HHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VITPASGFVGVQGAIIMGLLVSPVCYAAILLKGKLGYDDSLDAFGIHGVGGALGAVLTGV
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
FTLTLGAGVASKGDQILVQLISVAATGAYSFIVSMILVFLIDKTIGFRISEEKEITGLDS
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCC
EIHGEKGYIL
HHCCCCCCCC
>Mature Secondary Structure 
GIGMIMKWTKRLLLLLTLLLPFVLFGQEASTPVADKGDTVWMIVASALVFFMIPGLALF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YGGLVRSKNVLSTMMHSFVAILVLTLQWTIFGYSFAFSGTNPYFGNFDLAFLNGIDENTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEECCCCCCEE
ELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIIFILLWSTLVYDPVAHWVWAAD
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
GWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPALIAPNNLTYTLIGAGLLWF
CEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEHHHHHHHHH
GWFGFNAGSGLAVNGITARAFLVTLIAPAAAGVAWLVIEYFHTKKATALGAASGIVAGLV
HHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VITPASGFVGVQGAIIMGLLVSPVCYAAILLKGKLGYDDSLDAFGIHGVGGALGAVLTGV
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
FTLTLGAGVASKGDQILVQLISVAATGAYSFIVSMILVFLIDKTIGFRISEEKEITGLDS
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCC
EIHGEKGYIL
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]