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The map label for this gene is aspS

Identifier: 37521497

GI number: 37521497

Start: 2050434

End: 2052221

Strand: Reverse

Name: aspS

Synonym: gvip266

Alternate gene names: 37521497

Gene position: 2052221-2050434 (Counterclockwise)

Preceding gene: 37521500

Following gene: 37521493

Centisome position: 44.05

GC content: 63.98

Gene sequence:

>1788_bases
ATGCGTTCCGACTACTGCGGCACTTTGCGCTCCGAGCACATCGGCAAAACTGTCTCCCTCTACGGCTGGATCGACGGCTG
GCGCGATCACGGCGGCGTCATTTTTCTCGACCTGCGCGATTACACAGGTATCGTTCAAATTGTCGCCGACCCGCAGCGCA
CCCCCGAGAGCTACCACCTCGCCAGTTCTCTGCGCAACGAGTACGTCGTGCGCGTCGAAGGCCGGGTGAGCGCCCGCCCC
GAGCACTCCCTCAATCCGCGCCTTTCTACCGGCACAGTCGAAGTCTACGCCGATACCCTCGCAGTGCTCAACCGGGCCGA
GACCCCGCCCTTTGCGATCTCCAAGGACGAGGAAGTCGACGAGAAATTGCGCCTCAAGTTTCGCTATCTCGATTTGCGCC
GCGGGCGGATGCAAAAACTGTTGCGCCTGCGCCATCGGGTCATGCAGATTATGCGCCGCCACCTCGATGAGCGCGGGTTT
ACCGAGATCGAGACGCCTGTCCTGGTCAAATCCACCCCCGAAGGGGCGCGCGACTACCTGGTGCCCTCGCGGGTCAACCC
CGGCGATTGGTTCGCCCTGCCGCAGTCGCCGCAGCTATTCAAGCAACTGTTGATGGTCGCGGGCTTCGACCGCTACTACC
AGATCGCCCGCTGCTTCCGCGACGAAGATCTGCGCGCCGACCGCCAGCCGGAATTTACCCAGCTCGACATGGAAATGAGC
TTCCTGTCGATGGAAGAAATCATCGCCCTCAACGAAGGGTTGGTGGCCGCCATTCTCCAGGAGACGATGGGTCTGGAGCT
ATCTTTGCCGCTGCCGCGCCTTACCTACGCCGAGGCGATGGTCCGCTACGGCTCCGACAAGCCCGACACCCGCTTCGGCC
TCGAACTGGTCGAGGTCTCGGAGGTATTTCGCGAATCGAGTTTCCAGGTGCTCTCGGGGGCGGTGGCCGCCGGAGGCAAA
GTCGTCTGTCTGCCGGTACCGGGGGCCGAGGGGATCACCAATACCCGCGTCAAACCGGGGGGGGATCTTTTCGAGTTCGT
CACCCAGTTCGGGGCGCGGGGGCTGCTGTTTGCCCGCGTGCGCGAGGGCGGCCAGGTCGATACGATCGGGGCCTTCAGCA
AGAGCCTCACCCCCGAAATCGCCGCGGGCATGATCGAGAAGACCGGCGCCCAACCGGGCCATCTGCTGCTGTTTGGAGCG
GGCGATAGGACGGCGGTCCCGACCGTTCTCGATTACATGGGCCGCTTGCGCCTCAAGATGGGCGAAGAGTTGGGCCTCAT
CGACCCGAACCGTCACAACCTGCTCTGGGTGACCGACTTTCCGATGTTTGAATGGAACGCCGAGGAGAAGCGCCTCGAAG
CGCTCCACCACCCTTTTACCGCGCCGCGGCCCGAGGACGAGCACGATCTTAAGACCGCCCGCGCCCTCGCCTACGACATC
ATCTGGAACGGCGTCGAGGTGGGCGGCGGCAGCCTGCGCATCTACCGGCGCGCTCTGCAGGAACGGGTCTTTGAAACCAT
TGGCCTGACCGAGGAGGAGGCGCGCGCCAAATTCGGCTTCCTGCTCGACGCTTTTGAGTACGGCACCCCGCCCCACGGCG
GCATCGCCTACGGCTTCGACCGCTTCGTGATGCTCATTGCCGGCGAGCAATCGATCCGCGAGGTGATCGCCTTCCCGAAG
ACCCAGCGCGCCCAGGACCTGATGCTCGGCGCCCCGTCGGCCGTAGCTGAGCGCCAACTCAAGGAACTGAATGTGCGCTC
CACCCTGCCGCCCAAAACCCAGGGCTAA

Upstream 100 bases:

>100_bases
TTTCTGTCGAACGAACCGGCCGCTCCTGGGCAGCCGATGCCGAAGATAAAGGTTTGTGACTGCCCGGGTCTATCATGGAA
AAACTGCACTCGCTTCTTCC

Downstream 100 bases:

>100_bases
AGCGCTTATTCAAGTCGAAGGCTGAAAGTTCACTAGTGCATTCTTCGCTCTCATCAGACTCGGGGAGTAGTTAACAGCAT
TAGTGAGCTTCCAGTGTTGA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MRSDYCGTLRSEHIGKTVSLYGWIDGWRDHGGVIFLDLRDYTGIVQIVADPQRTPESYHLASSLRNEYVVRVEGRVSARP
EHSLNPRLSTGTVEVYADTLAVLNRAETPPFAISKDEEVDEKLRLKFRYLDLRRGRMQKLLRLRHRVMQIMRRHLDERGF
TEIETPVLVKSTPEGARDYLVPSRVNPGDWFALPQSPQLFKQLLMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDMEMS
FLSMEEIIALNEGLVAAILQETMGLELSLPLPRLTYAEAMVRYGSDKPDTRFGLELVEVSEVFRESSFQVLSGAVAAGGK
VVCLPVPGAEGITNTRVKPGGDLFEFVTQFGARGLLFARVREGGQVDTIGAFSKSLTPEIAAGMIEKTGAQPGHLLLFGA
GDRTAVPTVLDYMGRLRLKMGEELGLIDPNRHNLLWVTDFPMFEWNAEEKRLEALHHPFTAPRPEDEHDLKTARALAYDI
IWNGVEVGGGSLRIYRRALQERVFETIGLTEEEARAKFGFLLDAFEYGTPPHGGIAYGFDRFVMLIAGEQSIREVIAFPK
TQRAQDLMLGAPSAVAERQLKELNVRSTLPPKTQG

Sequences:

>Translated_595_residues
MRSDYCGTLRSEHIGKTVSLYGWIDGWRDHGGVIFLDLRDYTGIVQIVADPQRTPESYHLASSLRNEYVVRVEGRVSARP
EHSLNPRLSTGTVEVYADTLAVLNRAETPPFAISKDEEVDEKLRLKFRYLDLRRGRMQKLLRLRHRVMQIMRRHLDERGF
TEIETPVLVKSTPEGARDYLVPSRVNPGDWFALPQSPQLFKQLLMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDMEMS
FLSMEEIIALNEGLVAAILQETMGLELSLPLPRLTYAEAMVRYGSDKPDTRFGLELVEVSEVFRESSFQVLSGAVAAGGK
VVCLPVPGAEGITNTRVKPGGDLFEFVTQFGARGLLFARVREGGQVDTIGAFSKSLTPEIAAGMIEKTGAQPGHLLLFGA
GDRTAVPTVLDYMGRLRLKMGEELGLIDPNRHNLLWVTDFPMFEWNAEEKRLEALHHPFTAPRPEDEHDLKTARALAYDI
IWNGVEVGGGSLRIYRRALQERVFETIGLTEEEARAKFGFLLDAFEYGTPPHGGIAYGFDRFVMLIAGEQSIREVIAFPK
TQRAQDLMLGAPSAVAERQLKELNVRSTLPPKTQG
>Mature_595_residues
MRSDYCGTLRSEHIGKTVSLYGWIDGWRDHGGVIFLDLRDYTGIVQIVADPQRTPESYHLASSLRNEYVVRVEGRVSARP
EHSLNPRLSTGTVEVYADTLAVLNRAETPPFAISKDEEVDEKLRLKFRYLDLRRGRMQKLLRLRHRVMQIMRRHLDERGF
TEIETPVLVKSTPEGARDYLVPSRVNPGDWFALPQSPQLFKQLLMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDMEMS
FLSMEEIIALNEGLVAAILQETMGLELSLPLPRLTYAEAMVRYGSDKPDTRFGLELVEVSEVFRESSFQVLSGAVAAGGK
VVCLPVPGAEGITNTRVKPGGDLFEFVTQFGARGLLFARVREGGQVDTIGAFSKSLTPEIAAGMIEKTGAQPGHLLLFGA
GDRTAVPTVLDYMGRLRLKMGEELGLIDPNRHNLLWVTDFPMFEWNAEEKRLEALHHPFTAPRPEDEHDLKTARALAYDI
IWNGVEVGGGSLRIYRRALQERVFETIGLTEEEARAKFGFLLDAFEYGTPPHGGIAYGFDRFVMLIAGEQSIREVIAFPK
TQRAQDLMLGAPSAVAERQLKELNVRSTLPPKTQG

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=612, Percent_Identity=40.1960784313725, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI45439306, Length=291, Percent_Identity=27.8350515463918, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI5031815, Length=257, Percent_Identity=28.0155642023346, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI194272210, Length=257, Percent_Identity=28.0155642023346, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI4758762, Length=288, Percent_Identity=27.4305555555556, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI226491502, Length=255, Percent_Identity=24.3137254901961, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=48.2293423271501, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1790571, Length=297, Percent_Identity=25.9259259259259, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=25.2525252525253, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=608, Percent_Identity=34.703947368421, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI71994340, Length=146, Percent_Identity=30.1369863013699, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=146, Percent_Identity=30.1369863013699, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=146, Percent_Identity=30.1369863013699, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=250, Percent_Identity=28.8, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=302, Percent_Identity=38.0794701986755, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321807, Length=264, Percent_Identity=27.6515151515151, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=247, Percent_Identity=30.3643724696356, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=154, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=257, Percent_Identity=26.4591439688716, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24584738, Length=601, Percent_Identity=39.1014975041597, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=247, Percent_Identity=29.1497975708502, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=26.2745098039216, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=26.2745098039216, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19921528, Length=231, Percent_Identity=29.8701298701299, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_GLOVI (Q7NJA4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924874.1
- ProteinModelPortal:   Q7NJA4
- GeneID:   2600330
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1928
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- BioCyc:   GVIO251221:GLL1928-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66974; Mature: 66974

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDYCGTLRSEHIGKTVSLYGWIDGWRDHGGVIFLDLRDYTGIVQIVADPQRTPESYHL
CCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHH
ASSLRNEYVVRVEGRVSARPEHSLNPRLSTGTVEVYADTLAVLNRAETPPFAISKDEEVD
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
EKLRLKFRYLDLRRGRMQKLLRLRHRVMQIMRRHLDERGFTEIETPVLVKSTPEGARDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCC
VPSRVNPGDWFALPQSPQLFKQLLMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDMEMS
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHH
FLSMEEIIALNEGLVAAILQETMGLELSLPLPRLTYAEAMVRYGSDKPDTRFGLELVEVS
HHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EVFRESSFQVLSGAVAAGGKVVCLPVPGAEGITNTRVKPGGDLFEFVTQFGARGLLFARV
HHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEC
REGGQVDTIGAFSKSLTPEIAAGMIEKTGAQPGHLLLFGAGDRTAVPTVLDYMGRLRLKM
CCCCCEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHC
GEELGLIDPNRHNLLWVTDFPMFEWNAEEKRLEALHHPFTAPRPEDEHDLKTARALAYDI
CCCCCCCCCCCCCEEEEECCCCEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IWNGVEVGGGSLRIYRRALQERVFETIGLTEEEARAKFGFLLDAFEYGTPPHGGIAYGFD
HHCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCHH
RFVMLIAGEQSIREVIAFPKTQRAQDLMLGAPSAVAERQLKELNVRSTLPPKTQG
HEEEEEECCHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRSDYCGTLRSEHIGKTVSLYGWIDGWRDHGGVIFLDLRDYTGIVQIVADPQRTPESYHL
CCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHH
ASSLRNEYVVRVEGRVSARPEHSLNPRLSTGTVEVYADTLAVLNRAETPPFAISKDEEVD
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
EKLRLKFRYLDLRRGRMQKLLRLRHRVMQIMRRHLDERGFTEIETPVLVKSTPEGARDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCC
VPSRVNPGDWFALPQSPQLFKQLLMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDMEMS
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHH
FLSMEEIIALNEGLVAAILQETMGLELSLPLPRLTYAEAMVRYGSDKPDTRFGLELVEVS
HHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EVFRESSFQVLSGAVAAGGKVVCLPVPGAEGITNTRVKPGGDLFEFVTQFGARGLLFARV
HHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEC
REGGQVDTIGAFSKSLTPEIAAGMIEKTGAQPGHLLLFGAGDRTAVPTVLDYMGRLRLKM
CCCCCEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHC
GEELGLIDPNRHNLLWVTDFPMFEWNAEEKRLEALHHPFTAPRPEDEHDLKTARALAYDI
CCCCCCCCCCCCCEEEEECCCCEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IWNGVEVGGGSLRIYRRALQERVFETIGLTEEEARAKFGFLLDAFEYGTPPHGGIAYGFD
HHCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCHH
RFVMLIAGEQSIREVIAFPKTQRAQDLMLGAPSAVAERQLKELNVRSTLPPKTQG
HEEEEEECCHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292