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The map label for this gene is yfcG [H]

Identifier: 37521271

GI number: 37521271

Start: 1810644

End: 1811348

Strand: Reverse

Name: yfcG [H]

Synonym: gll1702

Alternate gene names: 37521271

Gene position: 1811348-1810644 (Counterclockwise)

Preceding gene: 37521272

Following gene: 37521269

Centisome position: 38.88

GC content: 61.42

Gene sequence:

>705_bases
ATGATCGACCTGTACTACTGGCCGACGCCCAACGGGCACAAGATCACCATTTTTCTGGAGGAAGCGGGCCTGGAGTACCG
GATTGTGCCGGTGAATATCGGCACCGGGGACCAATTCAAGCCCGCATTTTTGCAGATTGCACCCAACAACCGCATGCCCG
CCATCGTCGACAGCGCACCCGGCGAGGGCGGCGAAGCGGTTTCGGTGTTTGAGTCGGGGGCGATTTTGCTCTACCTGGCC
GAGAAGACCGGCCAATTTTTGCCCGCCGACCTGCGCGGGCGCAAGACGGCCCTGGAGTGGCTGTTCTGGCAGATGGGCGG
GCTCGGGCCAATGGCGGGGCAGAACCACCACTTCGGGGTCTACGCTCCTGAAAAGATTCCCTACGCCATCGAGCGCTACG
TCAAAGAAACCAACCGCCTCTACGGCGTCCTCGACCGGCACCTTGCCGGGCGTGACTTTCTGGCGGGCGACTATTCGATT
GCCGACATGGCCTGCTATCCCTGGATCGTGCCCTACGAGCGCCAGCAACAAAATCTCGAAGACTTTGCCGACCTCAAGCG
CTGGTTCGCCCGCATTGGTGAGCGCCCCGCGGTGATGCGCGCCTACGAAAAGGGCAAAGCGCTTGCCACTCAACCCACCG
TTACCGAGGAAAGCCGCAAAATCCTCTTCGGCCAGAGCGCAACCAGCGTCGCGCCACAAACTTGA

Upstream 100 bases:

>100_bases
CATCGGCCGCCCCTCACGATAAATTACCCTCCGCCCAAAAGGTCACCCAAGCCAAGGCTAGACTCGATTATGAGCAACGG
ACCGTTTCGGGGGCAAGCGC

Downstream 100 bases:

>100_bases
TTAGAGGGCAGCGAGCTGCATTTCCCAGCCGGGGAGCACCTCCGGCAGGCTCAGCACCTGGCCGAAGCCTAGGGTGATCA
CCTGGCCCTTGCGAAAAACG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MIDLYYWPTPNGHKITIFLEEAGLEYRIVPVNIGTGDQFKPAFLQIAPNNRMPAIVDSAPGEGGEAVSVFESGAILLYLA
EKTGQFLPADLRGRKTALEWLFWQMGGLGPMAGQNHHFGVYAPEKIPYAIERYVKETNRLYGVLDRHLAGRDFLAGDYSI
ADMACYPWIVPYERQQQNLEDFADLKRWFARIGERPAVMRAYEKGKALATQPTVTEESRKILFGQSATSVAPQT

Sequences:

>Translated_234_residues
MIDLYYWPTPNGHKITIFLEEAGLEYRIVPVNIGTGDQFKPAFLQIAPNNRMPAIVDSAPGEGGEAVSVFESGAILLYLA
EKTGQFLPADLRGRKTALEWLFWQMGGLGPMAGQNHHFGVYAPEKIPYAIERYVKETNRLYGVLDRHLAGRDFLAGDYSI
ADMACYPWIVPYERQQQNLEDFADLKRWFARIGERPAVMRAYEKGKALATQPTVTEESRKILFGQSATSVAPQT
>Mature_234_residues
MIDLYYWPTPNGHKITIFLEEAGLEYRIVPVNIGTGDQFKPAFLQIAPNNRMPAIVDSAPGEGGEAVSVFESGAILLYLA
EKTGQFLPADLRGRKTALEWLFWQMGGLGPMAGQNHHFGVYAPEKIPYAIERYVKETNRLYGVLDRHLAGRDFLAGDYSI
ADMACYPWIVPYERQQQNLEDFADLKRWFARIGERPAVMRAYEKGKALATQPTVTEESRKILFGQSATSVAPQT

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=207, Percent_Identity=58.9371980676328, Blast_Score=256, Evalue=6e-70,
Organism=Escherichia coli, GI87082195, Length=214, Percent_Identity=43.9252336448598, Blast_Score=162, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324100, Length=232, Percent_Identity=33.1896551724138, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17864592, Length=193, Percent_Identity=29.0155440414508, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17933730, Length=210, Percent_Identity=28.0952380952381, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI85725204, Length=194, Percent_Identity=28.3505154639175, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17737923, Length=194, Percent_Identity=28.3505154639175, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864594, Length=195, Percent_Identity=27.6923076923077, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=25.2873563218391, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549270, Length=195, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864598, Length=206, Percent_Identity=26.2135922330097, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26275; Mature: 26275

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYYWPTPNGHKITIFLEEAGLEYRIVPVNIGTGDQFKPAFLQIAPNNRMPAIVDSAP
CEEEEECCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEEECCC
GEGGEAVSVFESGAILLYLAEKTGQFLPADLRGRKTALEWLFWQMGGLGPMAGQNHHFGV
CCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
YAPEKIPYAIERYVKETNRLYGVLDRHLAGRDFLAGDYSIADMACYPWIVPYERQQQNLE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCEECCCHHHHCCHH
DFADLKRWFARIGERPAVMRAYEKGKALATQPTVTEESRKILFGQSATSVAPQT
HHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MIDLYYWPTPNGHKITIFLEEAGLEYRIVPVNIGTGDQFKPAFLQIAPNNRMPAIVDSAP
CEEEEECCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEEECCC
GEGGEAVSVFESGAILLYLAEKTGQFLPADLRGRKTALEWLFWQMGGLGPMAGQNHHFGV
CCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
YAPEKIPYAIERYVKETNRLYGVLDRHLAGRDFLAGDYSIADMACYPWIVPYERQQQNLE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCEECCCHHHHCCHH
DFADLKRWFARIGERPAVMRAYEKGKALATQPTVTEESRKILFGQSATSVAPQT
HHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]