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The map label for this gene is cspD [H]

Identifier: 34498661

GI number: 34498661

Start: 3489110

End: 3489442

Strand: Direct

Name: cspD [H]

Synonym: CV_3206

Alternate gene names: 34498661

Gene position: 3489110-3489442 (Clockwise)

Preceding gene: 34498659

Following gene: 34498662

Centisome position: 73.44

GC content: 52.55

Gene sequence:

>333_bases
ATGCCTCCCGCAGGATGCTCAAGTTGTGTGATGTGTAGTTGTCGTAGTGGCAATAGCCGTACCTCAGTCGCCATTTCCCT
TGATCTTCGTGCCTTTTATTCAACTGCTCGTTTTGAGGAATTTAACAAAATGGCAACCGGTACCGTTAAGTGGTTTAACG
ACTCCAAAGGCTTTGGCTTCATCACCCCGGACGAAGGCGGCGACGACGTATTTGCTCACTTCTCCCAAATCAACGCCAAG
GGCTTCCGCTCCCTGGCCGAAAACCAGCGCGTGAGCTTCGACATCGTCGAAGGCCCGAAGGGCAAGCAAGCCTCCAACAT
CCAGCCCATCTAA

Upstream 100 bases:

>100_bases
TTCCACCCAATGTAAATATCCTGTCCAAACGTATTAAAGATTCGTAAAAACAGACGATTCCTGAAGAAAACGCTTGCCAT
AGCGTGCAACTAGAGGCACT

Downstream 100 bases:

>100_bases
TCTCTTTCGAGAGATCATCTGGCAGTGATGACAAAACCCGGACCTGTCCGGGTTTTTTGCATTCTGATCGAGCCGATCCG
CGCCAGCCCTACAGGAGCCT

Product: cold shock transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MPPAGCSSCVMCSCRSGNSRTSVAISLDLRAFYSTARFEEFNKMATGTVKWFNDSKGFGFITPDEGGDDVFAHFSQINAK
GFRSLAENQRVSFDIVEGPKGKQASNIQPI

Sequences:

>Translated_110_residues
MPPAGCSSCVMCSCRSGNSRTSVAISLDLRAFYSTARFEEFNKMATGTVKWFNDSKGFGFITPDEGGDDVFAHFSQINAK
GFRSLAENQRVSFDIVEGPKGKQASNIQPI
>Mature_109_residues
PPAGCSSCVMCSCRSGNSRTSVAISLDLRAFYSTARFEEFNKMATGTVKWFNDSKGFGFITPDEGGDDVFAHFSQINAKG
FRSLAENQRVSFDIVEGPKGKQASNIQPI

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=56.7164179104478, Blast_Score=89, Evalue=8e-20,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=62.2950819672131, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=59.0909090909091, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=59.375, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=58.4615384615385, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=59.0909090909091, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1787839, Length=66, Percent_Identity=56.0606060606061, Blast_Score=75, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983225, Length=56, Percent_Identity=53.5714285714286, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983217, Length=56, Percent_Identity=53.5714285714286, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24658520, Length=69, Percent_Identity=43.4782608695652, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 11969; Mature: 11837

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAGCSSCVMCSCRSGNSRTSVAISLDLRAFYSTARFEEFNKMATGTVKWFNDSKGFGF
CCCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE
ITPDEGGDDVFAHFSQINAKGFRSLAENQRVSFDIVEGPKGKQASNIQPI
ECCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PPAGCSSCVMCSCRSGNSRTSVAISLDLRAFYSTARFEEFNKMATGTVKWFNDSKGFGF
CCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE
ITPDEGGDDVFAHFSQINAKGFRSLAENQRVSFDIVEGPKGKQASNIQPI
ECCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA