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The map label for this gene is livK [C]

Identifier: 34498277

GI number: 34498277

Start: 3072649

End: 3073797

Strand: Reverse

Name: livK [C]

Synonym: CV_2822

Alternate gene names: 34498277

Gene position: 3073797-3072649 (Counterclockwise)

Preceding gene: 34498278

Following gene: 34498276

Centisome position: 64.7

GC content: 66.41

Gene sequence:

>1149_bases
ATGGCTTGGAACAAACTGTTTGCGGAAATGAACTGGGTGGTGGCGCTGGGCGGCATGATGGCGCCGTTCGCCATGGCGGC
GGCCCATGCCGATACCGTGGTCAAGATAGGCTTCGCCTCGCCGCTGTCCGGCCCGCAGGCCCACTATGGCAAGGACAATG
AAAACGCCGCCAAAATGGCGGTGGACGATGTCAACGCCAAGGGCCTGGTGGTGGGCGGGCAGAAGATCAAGTTCGAACTG
GTGTCCGAGGACGACCAGGCCGACCCGCGCATCGGCACCCAGGCGGCGCAGCGGCTGGTGGACGCCGGCGTGAAAGCCGT
CATCGGCCACTTCAACTCCGGCGTGTCCATCCCGGCCTCGCGCCTGTATTCCGACGTCGGCATTCCGCAGCTGTCGGTGG
CCACCAACCCGGCCTATACCCAGCAAGGCTACAAAACCGCCTTCCGCCTGGTGGGCAGCGACAGCCAGATAGGCGGCGCG
CTGGGGCAGTTCGCCGTCAAAATGATGAAGGCCAAGAGCATCGTCGCCATCGACGACCGCACCGCCTACGGCCAGGGCAT
TGCCGACGAGTTCGTCAAGGCGGTGGCCGCCAACGGCGGCAAGGTGACGCGCCGCGAATTCACCACCGACAAGGCCACCG
ACTTCACCGCCATTCTCACCTCGCTCAAGGCCGTCAATCCGGACGTGGTGTTCTACGGCGGCGCCGACGCCCAGGCCGCG
CCCATGGTCAAGCAGATGAAGCGGTTGGGGCTCAAGGCCAAGCTGATGGGCGGCGACATGCTCAACACCCCCACCTTCAT
CCAACTGGCCGGCGCGGACGCGGCCGGCCACTACTCGGCGGTGGCCGGCGGCGTGCTGAGCGCCCGCCCGGCGGGCAAAG
AGTTCGAGCGCCGCTACAAGGCACGTTTCCATCAGGACGTGGTGCTGCTGGGGCCGCAATTCTACGACGGCGTGATGCTG
GTGGCCGCGGCCATGAAGCAGGCGGGCTCGGTGGAGCCGGCCAAATACCTGCCCAAGCTGGCCGCCATCCGCTACAGCGG
CGTGACGGCGGACTTCGCCTTCGCGCCCAACGGCAATCTGTTGAAAGCGCCGGTGACGCTGTCCACGGTGAAGAACAACG
CCTGGGCCGTGGAGACGGTGGTCCGCTGA

Upstream 100 bases:

>100_bases
ACCCGCGGCAGGCCGGCGCAGACCGGCCGCTGCGGGGGAGCGGTAGCAGGGCGGGCCGGGTGGCTTGCCCTGGTCAATGC
AGCAATAAAGGAGACATCTC

Downstream 100 bases:

>100_bases
GCGCGGCCAGGCAAAACGACATTTCCCCCGGCGTACTCCTCCGCCTTCGGGCCGGCTTTCTTCGTGCAGAGGCCGGCTTC
TTTTTTTATCGTTTGTCGGC

Product: amino acid ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MAWNKLFAEMNWVVALGGMMAPFAMAAAHADTVVKIGFASPLSGPQAHYGKDNENAAKMAVDDVNAKGLVVGGQKIKFEL
VSEDDQADPRIGTQAAQRLVDAGVKAVIGHFNSGVSIPASRLYSDVGIPQLSVATNPAYTQQGYKTAFRLVGSDSQIGGA
LGQFAVKMMKAKSIVAIDDRTAYGQGIADEFVKAVAANGGKVTRREFTTDKATDFTAILTSLKAVNPDVVFYGGADAQAA
PMVKQMKRLGLKAKLMGGDMLNTPTFIQLAGADAAGHYSAVAGGVLSARPAGKEFERRYKARFHQDVVLLGPQFYDGVML
VAAAMKQAGSVEPAKYLPKLAAIRYSGVTADFAFAPNGNLLKAPVTLSTVKNNAWAVETVVR

Sequences:

>Translated_382_residues
MAWNKLFAEMNWVVALGGMMAPFAMAAAHADTVVKIGFASPLSGPQAHYGKDNENAAKMAVDDVNAKGLVVGGQKIKFEL
VSEDDQADPRIGTQAAQRLVDAGVKAVIGHFNSGVSIPASRLYSDVGIPQLSVATNPAYTQQGYKTAFRLVGSDSQIGGA
LGQFAVKMMKAKSIVAIDDRTAYGQGIADEFVKAVAANGGKVTRREFTTDKATDFTAILTSLKAVNPDVVFYGGADAQAA
PMVKQMKRLGLKAKLMGGDMLNTPTFIQLAGADAAGHYSAVAGGVLSARPAGKEFERRYKARFHQDVVLLGPQFYDGVML
VAAAMKQAGSVEPAKYLPKLAAIRYSGVTADFAFAPNGNLLKAPVTLSTVKNNAWAVETVVR
>Mature_381_residues
AWNKLFAEMNWVVALGGMMAPFAMAAAHADTVVKIGFASPLSGPQAHYGKDNENAAKMAVDDVNAKGLVVGGQKIKFELV
SEDDQADPRIGTQAAQRLVDAGVKAVIGHFNSGVSIPASRLYSDVGIPQLSVATNPAYTQQGYKTAFRLVGSDSQIGGAL
GQFAVKMMKAKSIVAIDDRTAYGQGIADEFVKAVAANGGKVTRREFTTDKATDFTAILTSLKAVNPDVVFYGGADAQAAP
MVKQMKRLGLKAKLMGGDMLNTPTFIQLAGADAAGHYSAVAGGVLSARPAGKEFERRYKARFHQDVVLLGPQFYDGVMLV
AAAMKQAGSVEPAKYLPKLAAIRYSGVTADFAFAPNGNLLKAPVTLSTVKNNAWAVETVVR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=349, Percent_Identity=31.2320916905444, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI48994941, Length=244, Percent_Identity=31.9672131147541, Blast_Score=131, Evalue=6e-32,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40266; Mature: 40134

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWNKLFAEMNWVVALGGMMAPFAMAAAHADTVVKIGFASPLSGPQAHYGKDNENAAKMA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHEE
VDDVNAKGLVVGGQKIKFELVSEDDQADPRIGTQAAQRLVDAGVKAVIGHFNSGVSIPAS
EECCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RLYSDVGIPQLSVATNPAYTQQGYKTAFRLVGSDSQIGGALGQFAVKMMKAKSIVAIDDR
HHHHHCCCCEEEECCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCEEEECCC
TAYGQGIADEFVKAVAANGGKVTRREFTTDKATDFTAILTSLKAVNPDVVFYGGADAQAA
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHH
PMVKQMKRLGLKAKLMGGDMLNTPTFIQLAGADAAGHYSAVAGGVLSARPAGKEFERRYK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
ARFHQDVVLLGPQFYDGVMLVAAAMKQAGSVEPAKYLPKLAAIRYSGVTADFAFAPNGNL
HHHHCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCCEEEEEECCCCCE
LKAPVTLSTVKNNAWAVETVVR
EECCEEEEEECCCCEEEEEECC
>Mature Secondary Structure 
AWNKLFAEMNWVVALGGMMAPFAMAAAHADTVVKIGFASPLSGPQAHYGKDNENAAKMA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHEE
VDDVNAKGLVVGGQKIKFELVSEDDQADPRIGTQAAQRLVDAGVKAVIGHFNSGVSIPAS
EECCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RLYSDVGIPQLSVATNPAYTQQGYKTAFRLVGSDSQIGGALGQFAVKMMKAKSIVAIDDR
HHHHHCCCCEEEECCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCEEEECCC
TAYGQGIADEFVKAVAANGGKVTRREFTTDKATDFTAILTSLKAVNPDVVFYGGADAQAA
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHH
PMVKQMKRLGLKAKLMGGDMLNTPTFIQLAGADAAGHYSAVAGGVLSARPAGKEFERRYK
HHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
ARFHQDVVLLGPQFYDGVMLVAAAMKQAGSVEPAKYLPKLAAIRYSGVTADFAFAPNGNL
HHHHCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCCEEEEEECCCCCE
LKAPVTLSTVKNNAWAVETVVR
EECCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA