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The map label for this gene is dadA1 [H]

Identifier: 34498278

GI number: 34498278

Start: 3073898

End: 3075154

Strand: Reverse

Name: dadA1 [H]

Synonym: CV_2823

Alternate gene names: 34498278

Gene position: 3075154-3073898 (Counterclockwise)

Preceding gene: 34498279

Following gene: 34498277

Centisome position: 64.73

GC content: 72.08

Gene sequence:

>1257_bases
ATGGACGCTCTTGCCCCCGGCGCGGCCGTCATCGTGGTGGGCGCCGGCGTGGTCGGCATCGCCGCGGCCTTGCAGCTGCG
CCTGGCCGGTTTCGAGGTGGCGCTGCTGGACCGCGGCGAGCCGGCGATGGAAACCAGCTACGGCAATGCCGGCGCCTTCG
CCGTCAGCGACGTGATTCCGCTGGCCGAACCCGGCGTGCTGCGCAAGGTGCCGGGCTGGATGCTGGATCCGCTGGGGCCG
CTGGCCCTGCGCTGGCGCTACCTGCCCACGCTGGCGCCCTGGCTGCTGCGTTTCCTGGCCGCCAGCCGGCCGCAGCGGGT
GGCCGAGCTGACGCAGGCGCTGGCCGCGCTGCTGGGCCGGGTCAACGACGACTACGCGGCGCTGATAGAACGGGCCGGCT
TGGGCCATCTGTGGCGGCGGCACGGCAGCCTGACGCTGTACCGCAGCCGGGAGGAGTTCGACGCCGCCGCGCCGGCCTGG
GACGACAAGCGCCGCCATGGCGTGCGCTGGCTGGCGCTGGGCCGCGACGCGCTCGGCGAAGGCGAGCCGGTCTTGCGCGA
GGAATGGCAGTACGCGGTCCAGGTGCCGGCTTGGTCCCATGTGGACGACCCCTATGTTTTCAGCCGCGGCCTGTTCGACG
CCTTCCTCCAAGAAGGCGGCCGCTTTGTGCGGGACGAGGCGCTGGCCACGGCGATGGAAGCCGGCCGCGTCACTGGGGTA
GAGACGGCCGGCGGCGGCCGCCTGCGCGCCGACGCGGTGGTGATCGCCTGCGGCGTGTGGAGCGACCGCTTCGTCAAACA
GCACCGATACCGCGTGCCGCTGGAAAGCGAGCGCGGCTACCACGTCAACCTGCCCAAGGCCGGCGTGGCGCTGCGCCACT
TCATCCAGTGCGCCAGCGAGAGCTTCGTCATCCTGCCCATGGCCCATGGCGGCCTGCGCCTGGCCGGCACGGTGGAGCTG
GCGCACCGCGACGCGCCGCCGGACTGGCGCCGCGCCCACATCCTGCTGGACAAGGCCAGGCGCATCGTCGGCGATTTCTC
CACCGAAGACATGACGGTGTGGATGGGCAACCGCCCGTCGCTGCCTGACACCCTGCCCATCATCGGCCCTGCGCCGCTGC
CAGGGTTATGGTTCGCCACCGGCCACGGCCATTTGGGCCTGACCCTGGCAGCCACCACCGGCGCGCTGCTGCGCGACATG
CTGCAGGGCAGGGCGCCGGCGCTGGACATGCGGCCGTATCGGCTATCTCGTTTTTGA

Upstream 100 bases:

>100_bases
CTACCAGGACTTCCCGGACGCGCTGCTGCCGCCGGCATTGCGGCAGGCCAACCCGCTGGCGCTGCGCCGCCTGTTGGACG
GCCGGCGGGAGGGCTGAGTC

Downstream 100 bases:

>100_bases
ACCCGCGGCAGGCCGGCGCAGACCGGCCGCTGCGGGGGAGCGGTAGCAGGGCGGGCCGGGTGGCTTGCCCTGGTCAATGC
AGCAATAAAGGAGACATCTC

Product: D-amino-acid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MDALAPGAAVIVVGAGVVGIAAALQLRLAGFEVALLDRGEPAMETSYGNAGAFAVSDVIPLAEPGVLRKVPGWMLDPLGP
LALRWRYLPTLAPWLLRFLAASRPQRVAELTQALAALLGRVNDDYAALIERAGLGHLWRRHGSLTLYRSREEFDAAAPAW
DDKRRHGVRWLALGRDALGEGEPVLREEWQYAVQVPAWSHVDDPYVFSRGLFDAFLQEGGRFVRDEALATAMEAGRVTGV
ETAGGGRLRADAVVIACGVWSDRFVKQHRYRVPLESERGYHVNLPKAGVALRHFIQCASESFVILPMAHGGLRLAGTVEL
AHRDAPPDWRRAHILLDKARRIVGDFSTEDMTVWMGNRPSLPDTLPIIGPAPLPGLWFATGHGHLGLTLAATTGALLRDM
LQGRAPALDMRPYRLSRF

Sequences:

>Translated_418_residues
MDALAPGAAVIVVGAGVVGIAAALQLRLAGFEVALLDRGEPAMETSYGNAGAFAVSDVIPLAEPGVLRKVPGWMLDPLGP
LALRWRYLPTLAPWLLRFLAASRPQRVAELTQALAALLGRVNDDYAALIERAGLGHLWRRHGSLTLYRSREEFDAAAPAW
DDKRRHGVRWLALGRDALGEGEPVLREEWQYAVQVPAWSHVDDPYVFSRGLFDAFLQEGGRFVRDEALATAMEAGRVTGV
ETAGGGRLRADAVVIACGVWSDRFVKQHRYRVPLESERGYHVNLPKAGVALRHFIQCASESFVILPMAHGGLRLAGTVEL
AHRDAPPDWRRAHILLDKARRIVGDFSTEDMTVWMGNRPSLPDTLPIIGPAPLPGLWFATGHGHLGLTLAATTGALLRDM
LQGRAPALDMRPYRLSRF
>Mature_418_residues
MDALAPGAAVIVVGAGVVGIAAALQLRLAGFEVALLDRGEPAMETSYGNAGAFAVSDVIPLAEPGVLRKVPGWMLDPLGP
LALRWRYLPTLAPWLLRFLAASRPQRVAELTQALAALLGRVNDDYAALIERAGLGHLWRRHGSLTLYRSREEFDAAAPAW
DDKRRHGVRWLALGRDALGEGEPVLREEWQYAVQVPAWSHVDDPYVFSRGLFDAFLQEGGRFVRDEALATAMEAGRVTGV
ETAGGGRLRADAVVIACGVWSDRFVKQHRYRVPLESERGYHVNLPKAGVALRHFIQCASESFVILPMAHGGLRLAGTVEL
AHRDAPPDWRRAHILLDKARRIVGDFSTEDMTVWMGNRPSLPDTLPIIGPAPLPGLWFATGHGHLGLTLAATTGALLRDM
LQGRAPALDMRPYRLSRF

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=421, Percent_Identity=28.978622327791, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076
- InterPro:   IPR006036 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45687; Mature: 45687

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALAPGAAVIVVGAGVVGIAAALQLRLAGFEVALLDRGEPAMETSYGNAGAFAVSDVIP
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCCCCHHHCCCC
LAEPGVLRKVPGWMLDPLGPLALRWRYLPTLAPWLLRFLAASRPQRVAELTQALAALLGR
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VNDDYAALIERAGLGHLWRRHGSLTLYRSREEFDAAAPAWDDKRRHGVRWLALGRDALGE
CCCHHHHHHHHCCHHHHHHHCCCEEEEECHHHHCCCCCCCCCHHHCCCEEEEECCHHCCC
GEPVLREEWQYAVQVPAWSHVDDPYVFSRGLFDAFLQEGGRFVRDEALATAMEAGRVTGV
CCCHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECE
ETAGGGRLRADAVVIACGVWSDRFVKQHRYRVPLESERGYHVNLPKAGVALRHFIQCASE
EECCCCEEEECEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCC
SFVILPMAHGGLRLAGTVELAHRDAPPDWRRAHILLDKARRIVGDFSTEDMTVWMGNRPS
CEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
LPDTLPIIGPAPLPGLWFATGHGHLGLTLAATTGALLRDMLQGRAPALDMRPYRLSRF
CCCCCCCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MDALAPGAAVIVVGAGVVGIAAALQLRLAGFEVALLDRGEPAMETSYGNAGAFAVSDVIP
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCCCCHHHCCCC
LAEPGVLRKVPGWMLDPLGPLALRWRYLPTLAPWLLRFLAASRPQRVAELTQALAALLGR
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VNDDYAALIERAGLGHLWRRHGSLTLYRSREEFDAAAPAWDDKRRHGVRWLALGRDALGE
CCCHHHHHHHHCCHHHHHHHCCCEEEEECHHHHCCCCCCCCCHHHCCCEEEEECCHHCCC
GEPVLREEWQYAVQVPAWSHVDDPYVFSRGLFDAFLQEGGRFVRDEALATAMEAGRVTGV
CCCHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECE
ETAGGGRLRADAVVIACGVWSDRFVKQHRYRVPLESERGYHVNLPKAGVALRHFIQCASE
EECCCCEEEECEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCC
SFVILPMAHGGLRLAGTVELAHRDAPPDWRRAHILLDKARRIVGDFSTEDMTVWMGNRPS
CEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
LPDTLPIIGPAPLPGLWFATGHGHLGLTLAATTGALLRDMLQGRAPALDMRPYRLSRF
CCCCCCCCCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]