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The map label for this gene is chaA [C]

Identifier: 34498117

GI number: 34498117

Start: 2875693

End: 2876772

Strand: Reverse

Name: chaA [C]

Synonym: CV_2662

Alternate gene names: 34498117

Gene position: 2876772-2875693 (Counterclockwise)

Preceding gene: 34498122

Following gene: 34498116

Centisome position: 60.55

GC content: 63.61

Gene sequence:

>1080_bases
TTGATTCATAACACGATTCCGCGCTGGACCATTGTCACCCCTGTATTGGCCTGGGCCGCCATCGCCGCGTCTTCGGCGGC
GGGCGGGCATGCGCTCTATCTGGCGCTGCTGGCGGCGTTGCTGGCGGGTACGGTATTCGCCGCCGTGCATCATGCAGAGG
TGGTGGCGCATAAGGTGGGCGAGCCTTTCGGCACCCTGATCCTGGCGCTGGCGGTGACGGTGATCGAGGTGGCGCTGATC
ATTTCCTTCATGCTGGGCGGGGGCGAGGAGAAGCTGGCGCTGGCTCGGGACACCATTTTTTCCGCCATCATGATCACGTG
CAACGGCATTTTGGGCATTTGCCTGTTGCTGGGCGGACTGCGCCATCGCGAGCAGAACTTCCAGCTCAGCGGCGCCAAGG
CGGCGTTGACCGTGCTGGCGGCGATTTCGGTGCTGGCGCTGGTGCTGCCCAACTACGGCACCAGCCAGCCGGGCCCTTTG
CTGTCGTCGTCGCAGCTGGCGTTCACCGGCGTGGTGTCGCTGGTGCTGTACGGCGCTTTCGTGTTTGTGCAAACCATTCG
CCACCGCGATTATTTCTTGGTGGAAGGCAGCCATGACGAAGACGAGCACGCGCCGCCGCCTTCCAACAAGGTGGCTATGT
TGAGCGTGGGGGCGCTGCTGGTTTGCCTGGTGGCGGTGGTCACCCTGGCCAAGCTGCTGTCGCCGTCCATCGAGCGTTTC
GTGGTGGATGCCGGCGCGCCGGCGGCGGTGGTGGGCATCATCATCGCGTCCCTGGTGCTGCTGCCGGAGGGGCTGGCCGC
CGCCAACGCGGCGCGGGCGGATAAGGTGCAGACCAGCCTGAATCTGGCTTTGGGTTCGGCGCTGGCATCCATTGGCCTGA
CCATTCCCACGGTGGCGATGATTTTCGTGTTCATGGGCGAGCCGCTGATCCTGGGTTTGGAAAACAAGGAAACGATCTTT
CTGCTGCTGACGCTGGTGGCGTGCTCCTTGTCGCTGTCGGTCGGGCGCACCACCATTTTGCAGGGCATCGTGCACCTGGT
GATCTTCGCCTCTTTCGTATTCTTCGCGATCAGCCCCTGA

Upstream 100 bases:

>100_bases
TGGTTTCTCGATCGCTCTCCCAGAACGGTTGTCCCCTTGTTCCGGCCAAGCCTTTGCCCGGCGAGGCAGGCGGCTGTTCG
TCAACAATAAGGAAAATCCA

Downstream 100 bases:

>100_bases
GCGAATCACGGAGAGATAAAGCAAAAGCCGCCAGCATTGCGCTTGGCGGCTTTTCTGTTCCTGGCCTGCCGCTGGGCCCT
AAGCCCGGCGTTCAGCGTGA

Product: calcium/proton antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIHNTIPRWTIVTPVLAWAAIAASSAAGGHALYLALLAALLAGTVFAAVHHAEVVAHKVGEPFGTLILALAVTVIEVALI
ISFMLGGGEEKLALARDTIFSAIMITCNGILGICLLLGGLRHREQNFQLSGAKAALTVLAAISVLALVLPNYGTSQPGPL
LSSSQLAFTGVVSLVLYGAFVFVQTIRHRDYFLVEGSHDEDEHAPPPSNKVAMLSVGALLVCLVAVVTLAKLLSPSIERF
VVDAGAPAAVVGIIIASLVLLPEGLAAANAARADKVQTSLNLALGSALASIGLTIPTVAMIFVFMGEPLILGLENKETIF
LLLTLVACSLSLSVGRTTILQGIVHLVIFASFVFFAISP

Sequences:

>Translated_359_residues
MIHNTIPRWTIVTPVLAWAAIAASSAAGGHALYLALLAALLAGTVFAAVHHAEVVAHKVGEPFGTLILALAVTVIEVALI
ISFMLGGGEEKLALARDTIFSAIMITCNGILGICLLLGGLRHREQNFQLSGAKAALTVLAAISVLALVLPNYGTSQPGPL
LSSSQLAFTGVVSLVLYGAFVFVQTIRHRDYFLVEGSHDEDEHAPPPSNKVAMLSVGALLVCLVAVVTLAKLLSPSIERF
VVDAGAPAAVVGIIIASLVLLPEGLAAANAARADKVQTSLNLALGSALASIGLTIPTVAMIFVFMGEPLILGLENKETIF
LLLTLVACSLSLSVGRTTILQGIVHLVIFASFVFFAISP
>Mature_359_residues
MIHNTIPRWTIVTPVLAWAAIAASSAAGGHALYLALLAALLAGTVFAAVHHAEVVAHKVGEPFGTLILALAVTVIEVALI
ISFMLGGGEEKLALARDTIFSAIMITCNGILGICLLLGGLRHREQNFQLSGAKAALTVLAAISVLALVLPNYGTSQPGPL
LSSSQLAFTGVVSLVLYGAFVFVQTIRHRDYFLVEGSHDEDEHAPPPSNKVAMLSVGALLVCLVAVVTLAKLLSPSIERF
VVDAGAPAAVVGIIIASLVLLPEGLAAANAARADKVQTSLNLALGSALASIGLTIPTVAMIFVFMGEPLILGLENKETIF
LLLTLVACSLSLSVGRTTILQGIVHLVIFASFVFFAISP

Specific function: Possible cation transporter [H]

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=313, Percent_Identity=36.741214057508, Blast_Score=194, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 37247; Mature: 37247

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHNTIPRWTIVTPVLAWAAIAASSAAGGHALYLALLAALLAGTVFAAVHHAEVVAHKVG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPFGTLILALAVTVIEVALIISFMLGGGEEKLALARDTIFSAIMITCNGILGICLLLGGL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHREQNFQLSGAKAALTVLAAISVLALVLPNYGTSQPGPLLSSSQLAFTGVVSLVLYGAF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VFVQTIRHRDYFLVEGSHDEDEHAPPPSNKVAMLSVGALLVCLVAVVTLAKLLSPSIERF
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVDAGAPAAVVGIIIASLVLLPEGLAAANAARADKVQTSLNLALGSALASIGLTIPTVAM
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IFVFMGEPLILGLENKETIFLLLTLVACSLSLSVGRTTILQGIVHLVIFASFVFFAISP
HHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIHNTIPRWTIVTPVLAWAAIAASSAAGGHALYLALLAALLAGTVFAAVHHAEVVAHKVG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPFGTLILALAVTVIEVALIISFMLGGGEEKLALARDTIFSAIMITCNGILGICLLLGGL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHREQNFQLSGAKAALTVLAAISVLALVLPNYGTSQPGPLLSSSQLAFTGVVSLVLYGAF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VFVQTIRHRDYFLVEGSHDEDEHAPPPSNKVAMLSVGALLVCLVAVVTLAKLLSPSIERF
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVDAGAPAAVVGIIIASLVLLPEGLAAANAARADKVQTSLNLALGSALASIGLTIPTVAM
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IFVFMGEPLILGLENKETIFLLLTLVACSLSLSVGRTTILQGIVHLVIFASFVFFAISP
HHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]