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The map label for this gene is tig

Identifier: 34498014

GI number: 34498014

Start: 2753667

End: 2754974

Strand: Reverse

Name: tig

Synonym: CV_2559

Alternate gene names: 34498014

Gene position: 2754974-2753667 (Counterclockwise)

Preceding gene: 34498016

Following gene: 34498013

Centisome position: 57.99

GC content: 62.08

Gene sequence:

>1308_bases
ATGCAAGTACAGCTGGAAACTCTGAGCAATCTTGAGCGCCGCATGAACATCGCCCTGCCGATGGCAGCCATTGAAACCCA
AGTCGCCGAGCGTCTGAAGCGCGTGGCGCGCGGCGCCAAGATCCAAGGTTTCCGCCCGGGCAAGGCTCCGCTGAAGATCG
TGGAGATGAACTACGGCGCCCAGGTGCGTGAAGAAGTGATGGGTGAGCAGGTTCAGCAAGGCTTCTACAAGGCCGTGACC
GAGCAAAGCCTGCGCGTGGCCGGCTACCCGCGTTTCGAGCCGGTTGCCGCCGGCGACGACAAGGAAAGCTTCAAGTTCGC
CGCCACTTTCGAAATCTACCCGGAAGTGAAGGTCGGCGAGCTGGCAGGCAAGGAAATCGAAAAGCCGAATACCGTGGTCG
GCGACGCCGAGATCGAGAAGACCATCGACATCCTGCGCAAGCAGCGCACCCGCTACAACCGCGTTGAGCGCGAAGCTGCC
GAGGGCGACCGCGTGATCATCGACTTCAAGGGCGCCATCGACGGCGTGGCCTTCGAGGGCGGCTCTTCCGAGAACTTCCC
CTTCGTGCTGGGCCAGGGCCAGATGCTGGCCGAGTTCGAGGCCGGCATCGTCGGCGCCAAGGAAGGCGACATCAAGACCG
TGGAAGTGAACTTCCCCGAGGACTATCACGGCAAGGACGTGGCCGGCAAAACCGCCGTGTTCGAAATCCTGGTGAAGAAC
GTGGCTGAAGCCACCCTGCCGGAAGTGAACGAAGAGTTCGCCAAGGCGCTGGGCATCTCCGACGGCGACGTGGAAAAGAT
GCGCGGCGAAATCCGCAAGAACGTCGAGCGCGAAGTGAAGCGCCGCCTGCAGGCCCGCATCAAGGAAAACGTGATGCAGG
CGCTGATTGACGCCACCGAGCTGGAACTGCCGAAGGCCCTGGTGAGCCTGGAAATCGGCCGCCTGGTCGAGCAAGCCCGC
CGCGACATGCAGCAACGCGGCATGAACGTGAAGGACATGCCCTTCCCGCCGGAACTGTTCGCCGCCCAGGCCGAACGCCG
CGTGAAGCTGGGTCTGATCCTGTCCGAAATCGTGGAAGCCAACAAGCTGGAAGCCAAGCCGGAGCAGGTGCGCGCGATGA
TCACCGAGTTCGCCGACAGCTACGAGCAGCCGGAAGACGTGCTGGCCTGGTACTACGAGAGCGCCGACCGCCTCGAAGGC
CCGACTTCCATGGTCCTGGAAGACAATGTGGTTGAATTTGTGCTGTCTCAGGCCAATGTTGTAGCCAAGGACCTGTCCTT
CGACGAACTGATGGGCAGCCAAGCCTGA

Upstream 100 bases:

>100_bases
CGATTCCATGCCGTCCGGCGGGCGCCGGAAGCCTGGCGGGAATGGGCGTCGTACCGTCCCAGATGACTAAAAAACACCTG
TCACAGTTGAAGGAATCAAC

Downstream 100 bases:

>100_bases
TAACAACTTTCGGAGTGGCCGATGAAATCTATGATGGAACCGCAAGGTCTGGGCCTGGTGCCCATGGTGGTGGAGCAGAG
TGGCCGCGGCGAACGCGCCT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQVQLETLSNLERRMNIALPMAAIETQVAERLKRVARGAKIQGFRPGKAPLKIVEMNYGAQVREEVMGEQVQQGFYKAVT
EQSLRVAGYPRFEPVAAGDDKESFKFAATFEIYPEVKVGELAGKEIEKPNTVVGDAEIEKTIDILRKQRTRYNRVEREAA
EGDRVIIDFKGAIDGVAFEGGSSENFPFVLGQGQMLAEFEAGIVGAKEGDIKTVEVNFPEDYHGKDVAGKTAVFEILVKN
VAEATLPEVNEEFAKALGISDGDVEKMRGEIRKNVEREVKRRLQARIKENVMQALIDATELELPKALVSLEIGRLVEQAR
RDMQQRGMNVKDMPFPPELFAAQAERRVKLGLILSEIVEANKLEAKPEQVRAMITEFADSYEQPEDVLAWYYESADRLEG
PTSMVLEDNVVEFVLSQANVVAKDLSFDELMGSQA

Sequences:

>Translated_435_residues
MQVQLETLSNLERRMNIALPMAAIETQVAERLKRVARGAKIQGFRPGKAPLKIVEMNYGAQVREEVMGEQVQQGFYKAVT
EQSLRVAGYPRFEPVAAGDDKESFKFAATFEIYPEVKVGELAGKEIEKPNTVVGDAEIEKTIDILRKQRTRYNRVEREAA
EGDRVIIDFKGAIDGVAFEGGSSENFPFVLGQGQMLAEFEAGIVGAKEGDIKTVEVNFPEDYHGKDVAGKTAVFEILVKN
VAEATLPEVNEEFAKALGISDGDVEKMRGEIRKNVEREVKRRLQARIKENVMQALIDATELELPKALVSLEIGRLVEQAR
RDMQQRGMNVKDMPFPPELFAAQAERRVKLGLILSEIVEANKLEAKPEQVRAMITEFADSYEQPEDVLAWYYESADRLEG
PTSMVLEDNVVEFVLSQANVVAKDLSFDELMGSQA
>Mature_435_residues
MQVQLETLSNLERRMNIALPMAAIETQVAERLKRVARGAKIQGFRPGKAPLKIVEMNYGAQVREEVMGEQVQQGFYKAVT
EQSLRVAGYPRFEPVAAGDDKESFKFAATFEIYPEVKVGELAGKEIEKPNTVVGDAEIEKTIDILRKQRTRYNRVEREAA
EGDRVIIDFKGAIDGVAFEGGSSENFPFVLGQGQMLAEFEAGIVGAKEGDIKTVEVNFPEDYHGKDVAGKTAVFEILVKN
VAEATLPEVNEEFAKALGISDGDVEKMRGEIRKNVEREVKRRLQARIKENVMQALIDATELELPKALVSLEIGRLVEQAR
RDMQQRGMNVKDMPFPPELFAAQAERRVKLGLILSEIVEANKLEAKPEQVRAMITEFADSYEQPEDVLAWYYESADRLEG
PTSMVLEDNVVEFVLSQANVVAKDLSFDELMGSQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=438, Percent_Identity=40.6392694063927, Blast_Score=328, Evalue=5e-91,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CHRVO (Q7NUY8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902229.1
- ProteinModelPortal:   Q7NUY8
- SMR:   Q7NUY8
- GeneID:   2547231
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2559
- NMPDR:   fig|243365.1.peg.2559
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q7NUY8
- ProtClustDB:   PRK01490
- BioCyc:   CVIO243365:CV_2559-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48577; Mature: 48577

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVQLETLSNLERRMNIALPMAAIETQVAERLKRVARGAKIQGFRPGKAPLKIVEMNYGA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCH
QVREEVMGEQVQQGFYKAVTEQSLRVAGYPRFEPVAAGDDKESFKFAATFEIYPEVKVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEHH
LAGKEIEKPNTVVGDAEIEKTIDILRKQRTRYNRVEREAAEGDRVIIDFKGAIDGVAFEG
HCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECC
GSSENFPFVLGQGQMLAEFEAGIVGAKEGDIKTVEVNFPEDYHGKDVAGKTAVFEILVKN
CCCCCCCEEECCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
VAEATLPEVNEEFAKALGISDGDVEKMRGEIRKNVEREVKRRLQARIKENVMQALIDATE
HHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELPKALVSLEIGRLVEQARRDMQQRGMNVKDMPFPPELFAAQAERRVKLGLILSEIVEA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKLEAKPEQVRAMITEFADSYEQPEDVLAWYYESADRLEGPTSMVLEDNVVEFVLSQANV
HCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VAKDLSFDELMGSQA
HHHCCCHHHHCCCCC
>Mature Secondary Structure
MQVQLETLSNLERRMNIALPMAAIETQVAERLKRVARGAKIQGFRPGKAPLKIVEMNYGA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCH
QVREEVMGEQVQQGFYKAVTEQSLRVAGYPRFEPVAAGDDKESFKFAATFEIYPEVKVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEHH
LAGKEIEKPNTVVGDAEIEKTIDILRKQRTRYNRVEREAAEGDRVIIDFKGAIDGVAFEG
HCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECC
GSSENFPFVLGQGQMLAEFEAGIVGAKEGDIKTVEVNFPEDYHGKDVAGKTAVFEILVKN
CCCCCCCEEECCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
VAEATLPEVNEEFAKALGISDGDVEKMRGEIRKNVEREVKRRLQARIKENVMQALIDATE
HHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELPKALVSLEIGRLVEQARRDMQQRGMNVKDMPFPPELFAAQAERRVKLGLILSEIVEA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKLEAKPEQVRAMITEFADSYEQPEDVLAWYYESADRLEGPTSMVLEDNVVEFVLSQANV
HCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VAKDLSFDELMGSQA
HHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782