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The map label for this gene is narK1 [H]

Identifier: 34498000

GI number: 34498000

Start: 2738328

End: 2739611

Strand: Reverse

Name: narK1 [H]

Synonym: CV_2545

Alternate gene names: 34498000

Gene position: 2739611-2738328 (Counterclockwise)

Preceding gene: 34498001

Following gene: 34497999

Centisome position: 57.66

GC content: 66.51

Gene sequence:

>1284_bases
ATGACTTCCCCGACATTCAAACGCTACAGCGTGCTGGCCAGCAGCACGCTGGCTTTCACCCTCTGCTTCATGGTCTGGAT
GATGTTCGCGGTGCTGGGCATCCCGATCAAGCAGCAGCTGGGCCTCAATGAAACCGAGTTCGGCATCCTGGCCGCCACGC
CGGTGCTGACCGGTTCGCTGATCCGCGTGCCGCTGGGCATCTGGACCGACCGCTACGGCGGCCGCCCGGTGCTGTTCGCG
CTGATGCTCAGCTGCGTGCTGCCCATCTACCTGATGCAGTTCGCCAGCGCCTACTGGCAGTTCCTGCTGGCCGGGCTATT
CGTCGGCCTGGCCGGCGGTTCGTTCTCGGTCGGCACGCCCTATGTGGCGCGCTGGTTCCGCAAGGAGCAGCAGGGCCTGG
CCATGGGCATTTTCGGCGCCGGCAACGCCGGCTCGGCGCTGACCAAGCTGGTGGCCCCGGCGCTGATCCTGGCCTGGGGC
TGGCAGAGCGTGCCCACCGCCTACGCGCTGATCATGCTGGCCGCCGCCATCCTGTTCTGGCTGTTCTCCTTCAGCGATCC
GGCCCACAAGGCGCCGTCCACGGTGACGCTGCGCCAGCAGCTGGCGCTGATGAAGCATCCGGCGGTGCTGCGCTATTGCC
AGTACTACTCGGTGGTGTTCGGCGGCTACGTGGCGCTGGCGCTGTGGATGACCAAGTACTACGTCGGCGAGTACGGGCTG
GACCTGAAGCACGCGGCGTTGCTGGCGGCCTGTTTCTCCTTGCCGGGCGGCGTGCTGCGCGCGGTGGGGGGCTATCTGTC
TGACCGCTTCGGCCCCTACGTGGTCACTTGGGCGGTGATGTGGGTGTGCTGGGTGGCCTTCTTCCTGCTGTCCTATCCGC
AGACCGAGATGCGCGTGGCGACCACCGGCGGGCCGCTCAGCCTGCACATCGGCCTCAACGTCTACCTGTTCACCGGCCTG
CTGTTCCTGGTGGGCGTGGCGATGGCGATAGGCAAGGCCTCGGTGTTCAAGTTCATCTCCGACGAATTTCCCGACTCCAT
CGGCGCGGTGTCCGGCGTGGTCGGCCTGTCCGGCGGCCTGGGCGGCTTCCTGCTGCCCATCCTGTTCGGCGTCTTGCTCG
ACCTGACCGGCGTGCGCAGCAGCGCCTTCATGCTGCTGTACGGCACGGTGTGCGTCAGCCTGGTGTGGATGCATTTTTCC
TTCAAATCGGCGGCAAGAGCCAAGGCGCGGGCGCGCGCGGCCGAGCCCGTCAATCCCATTCCCTTGACCAACGAACGCGG
CTGA

Upstream 100 bases:

>100_bases
CTCGTCCGTTGGCAGACTGACAGTCCTGCGACAGACGAATATCGGCGCGCGCCGCCGTCGCTTATCGTGGTAGCCATGAC
CACTCCATGGATATGGCGCC

Downstream 100 bases:

>100_bases
GCGCGGCGCGGGAGACACTGATGACTTATCTGATCAAACACTGGGAGCCGGAATCGGCTTCGTTCTGGCAGCAGCAGGGA
CGCTCGGTGGCGCGGCGCAA

Product: nitrite extrusion protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTSPTFKRYSVLASSTLAFTLCFMVWMMFAVLGIPIKQQLGLNETEFGILAATPVLTGSLIRVPLGIWTDRYGGRPVLFA
LMLSCVLPIYLMQFASAYWQFLLAGLFVGLAGGSFSVGTPYVARWFRKEQQGLAMGIFGAGNAGSALTKLVAPALILAWG
WQSVPTAYALIMLAAAILFWLFSFSDPAHKAPSTVTLRQQLALMKHPAVLRYCQYYSVVFGGYVALALWMTKYYVGEYGL
DLKHAALLAACFSLPGGVLRAVGGYLSDRFGPYVVTWAVMWVCWVAFFLLSYPQTEMRVATTGGPLSLHIGLNVYLFTGL
LFLVGVAMAIGKASVFKFISDEFPDSIGAVSGVVGLSGGLGGFLLPILFGVLLDLTGVRSSAFMLLYGTVCVSLVWMHFS
FKSAARAKARARAAEPVNPIPLTNERG

Sequences:

>Translated_427_residues
MTSPTFKRYSVLASSTLAFTLCFMVWMMFAVLGIPIKQQLGLNETEFGILAATPVLTGSLIRVPLGIWTDRYGGRPVLFA
LMLSCVLPIYLMQFASAYWQFLLAGLFVGLAGGSFSVGTPYVARWFRKEQQGLAMGIFGAGNAGSALTKLVAPALILAWG
WQSVPTAYALIMLAAAILFWLFSFSDPAHKAPSTVTLRQQLALMKHPAVLRYCQYYSVVFGGYVALALWMTKYYVGEYGL
DLKHAALLAACFSLPGGVLRAVGGYLSDRFGPYVVTWAVMWVCWVAFFLLSYPQTEMRVATTGGPLSLHIGLNVYLFTGL
LFLVGVAMAIGKASVFKFISDEFPDSIGAVSGVVGLSGGLGGFLLPILFGVLLDLTGVRSSAFMLLYGTVCVSLVWMHFS
FKSAARAKARARAAEPVNPIPLTNERG
>Mature_426_residues
TSPTFKRYSVLASSTLAFTLCFMVWMMFAVLGIPIKQQLGLNETEFGILAATPVLTGSLIRVPLGIWTDRYGGRPVLFAL
MLSCVLPIYLMQFASAYWQFLLAGLFVGLAGGSFSVGTPYVARWFRKEQQGLAMGIFGAGNAGSALTKLVAPALILAWGW
QSVPTAYALIMLAAAILFWLFSFSDPAHKAPSTVTLRQQLALMKHPAVLRYCQYYSVVFGGYVALALWMTKYYVGEYGLD
LKHAALLAACFSLPGGVLRAVGGYLSDRFGPYVVTWAVMWVCWVAFFLLSYPQTEMRVATTGGPLSLHIGLNVYLFTGLL
FLVGVAMAIGKASVFKFISDEFPDSIGAVSGVVGLSGGLGGFLLPILFGVLLDLTGVRSSAFMLLYGTVCVSLVWMHFSF
KSAARAKARARAAEPVNPIPLTNERG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=427, Percent_Identity=25.9953161592506, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787475, Length=430, Percent_Identity=25.5813953488372, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786686, Length=364, Percent_Identity=25, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI2367379, Length=259, Percent_Identity=25.0965250965251, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46208; Mature: 46077

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCC
>Mature Secondary Structure 
TSPTFKRYSVLASSTLAFTLCFMVWMMFAVLGIPIKQQLGLNETEFGILAATPVLTGSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECHHHHCHH
IRVPLGIWTDRYGGRPVLFALMLSCVLPIYLMQFASAYWQFLLAGLFVGLAGGSFSVGTP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
YVARWFRKEQQGLAMGIFGAGNAGSALTKLVAPALILAWGWQSVPTAYALIMLAAAILFW
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LFSFSDPAHKAPSTVTLRQQLALMKHPAVLRYCQYYSVVFGGYVALALWMTKYYVGEYGL
HHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLKHAALLAACFSLPGGVLRAVGGYLSDRFGPYVVTWAVMWVCWVAFFLLSYPQTEMRVA
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TTGGPLSLHIGLNVYLFTGLLFLVGVAMAIGKASVFKFISDEFPDSIGAVSGVVGLSGGL
ECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
GGFLLPILFGVLLDLTGVRSSAFMLLYGTVCVSLVWMHFSFKSAARAKARARAAEPVNPI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLTNERG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]