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The map label for this gene is folC [H]

Identifier: 34497973

GI number: 34497973

Start: 2710447

End: 2711712

Strand: Reverse

Name: folC [H]

Synonym: CV_2518

Alternate gene names: 34497973

Gene position: 2711712-2710447 (Counterclockwise)

Preceding gene: 34497974

Following gene: 34497972

Centisome position: 57.08

GC content: 66.67

Gene sequence:

>1266_bases
ATGACGAGAAAGACACTGGACGACTGGCTGAGCTGGCAGGAAAGCTTGCACACGGCGGCGATAGACATGGGCCTGGCCCG
GGTCGAACGCGTGCGCGGCGCGATGGGCCTCAAGCCCGCCTGTCCGGTGATCATGGTGGCCGGCACCAATGGCAAGGGCT
CCACCTGCGCGATGCTGTCCAGCATCTTCCGCGCCGCGGGCTTCAAGGTCGGCGCCTACACCTCGCCGCACATCCTGCGC
TACAACGAGCGCATCGCCATCGATCTCAAGCCGGCGTCCGACGAGCGCATCATCGCCAGCTTCGAGGCGATAGACGCCGC
GCGCGGCGACACCACGCTGACTTATTTCGAATTCGGCACCCTGGCCGCGGTGCACAGCTTCGTCGCCGAGAAGGTGGATG
TGATCATCCTGGAAGTGGGCCTGGGCGGACGGCTGGACGCGGTCAACATCTTCGAGCCGGATGTGTCCGTCGTCGTCAGC
GTGGATTTGGATCACCAGGCGATTCTGGGCGACAACCGCGAGGACATCGGTTTCGAGAAGGCCGGCGTCTACCGCGCCGG
CAAGCCGGCGATCTGCGTCGATCCCGAGCCGCCGCAGCGGCTGCTGGACCACGCGGCGGCCATCGGCGCGGATCTCAAAC
TGTACGGCCGCGACTTCGGCTACACCCGGATGGACAATCAGTGGTCGTTCCGCTGCGGCGACCACGCCCGCCACGCGCTG
CCCATCCCGGCATTGCGCGGCGCTTACCAGATGATCAACGCCTGCGGCGCGCTGGCGGTTCTGGACGCGATCCGCGACAA
GCTGCCGGTTGGCATCGGCGCGATCAAGCAGGGCCTGGTGGAGGTGGAGTGGCCGGGCCGGTTCCAGGTGCTGCCGGGCC
GTCCGGCGGTGGTGCTGGATGTCGGCCACAATCCGCACGCGGTCAAGGCGATGGCGGCGGCGCTCAAGCAGCTGCCGTTC
GCGGAAAACCGCTACGCGGTGTTCTCCATGCTGGCGGACAAGGACATGATGGCGGTGGTCGAGCTGGCCAAGGGCGAGTT
CGACCACTGGCTGGTCGCCGGGCTGGACATGCCGCGCGGGCAGAGCGGCGAGGCCATTGCCTCTGCGCTGAAGAATGCTG
GCATCGCGTCGGTGTCCGCCTTCGATACCGTCGCCCAGGCGTGGTCGGCCGCCTTATCGCGGGCCGGCGACAATGATAGA
ATCGTTGTTTTCGGATCATTCCACACCGTAGCGGAAGTGGAAGCGGTCCGGCGTCACCCGGTTTGA

Upstream 100 bases:

>100_bases
CGGATTCGGCGGATGCCGACGCGCGGCGGCGACTCAGGGTTTACCTGCTGTGGTTCCATCCGCCGCAGCCTACGCAAGAA
GATACGGAAATCACCCGATG

Downstream 100 bases:

>100_bases
GGAATGCATGGCACTGTCCAGCCACGATGAACTGATATTGCTGAGAAAGCGCGCCCGCCGCCGCTTGGTGGGAGCCGTGG
TGCTGGTTTCCATCGCCACC

Product: folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTRKTLDDWLSWQESLHTAAIDMGLARVERVRGAMGLKPACPVIMVAGTNGKGSTCAMLSSIFRAAGFKVGAYTSPHILR
YNERIAIDLKPASDERIIASFEAIDAARGDTTLTYFEFGTLAAVHSFVAEKVDVIILEVGLGGRLDAVNIFEPDVSVVVS
VDLDHQAILGDNREDIGFEKAGVYRAGKPAICVDPEPPQRLLDHAAAIGADLKLYGRDFGYTRMDNQWSFRCGDHARHAL
PIPALRGAYQMINACGALAVLDAIRDKLPVGIGAIKQGLVEVEWPGRFQVLPGRPAVVLDVGHNPHAVKAMAAALKQLPF
AENRYAVFSMLADKDMMAVVELAKGEFDHWLVAGLDMPRGQSGEAIASALKNAGIASVSAFDTVAQAWSAALSRAGDNDR
IVVFGSFHTVAEVEAVRRHPV

Sequences:

>Translated_421_residues
MTRKTLDDWLSWQESLHTAAIDMGLARVERVRGAMGLKPACPVIMVAGTNGKGSTCAMLSSIFRAAGFKVGAYTSPHILR
YNERIAIDLKPASDERIIASFEAIDAARGDTTLTYFEFGTLAAVHSFVAEKVDVIILEVGLGGRLDAVNIFEPDVSVVVS
VDLDHQAILGDNREDIGFEKAGVYRAGKPAICVDPEPPQRLLDHAAAIGADLKLYGRDFGYTRMDNQWSFRCGDHARHAL
PIPALRGAYQMINACGALAVLDAIRDKLPVGIGAIKQGLVEVEWPGRFQVLPGRPAVVLDVGHNPHAVKAMAAALKQLPF
AENRYAVFSMLADKDMMAVVELAKGEFDHWLVAGLDMPRGQSGEAIASALKNAGIASVSAFDTVAQAWSAALSRAGDNDR
IVVFGSFHTVAEVEAVRRHPV
>Mature_420_residues
TRKTLDDWLSWQESLHTAAIDMGLARVERVRGAMGLKPACPVIMVAGTNGKGSTCAMLSSIFRAAGFKVGAYTSPHILRY
NERIAIDLKPASDERIIASFEAIDAARGDTTLTYFEFGTLAAVHSFVAEKVDVIILEVGLGGRLDAVNIFEPDVSVVVSV
DLDHQAILGDNREDIGFEKAGVYRAGKPAICVDPEPPQRLLDHAAAIGADLKLYGRDFGYTRMDNQWSFRCGDHARHALP
IPALRGAYQMINACGALAVLDAIRDKLPVGIGAIKQGLVEVEWPGRFQVLPGRPAVVLDVGHNPHAVKAMAAALKQLPFA
ENRYAVFSMLADKDMMAVVELAKGEFDHWLVAGLDMPRGQSGEAIASALKNAGIASVSAFDTVAQAWSAALSRAGDNDRI
VVFGSFHTVAEVEAVRRHPV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=338, Percent_Identity=32.8402366863905, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI66932990, Length=338, Percent_Identity=32.8402366863905, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788654, Length=412, Percent_Identity=44.4174757281553, Blast_Score=325, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17553150, Length=292, Percent_Identity=32.8767123287671, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=292, Percent_Identity=32.8767123287671, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=292, Percent_Identity=32.8767123287671, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=26.1792452830189, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324815, Length=179, Percent_Identity=37.9888268156425, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24641571, Length=272, Percent_Identity=32.3529411764706, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=36.8715083798883, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45308; Mature: 45177

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKTLDDWLSWQESLHTAAIDMGLARVERVRGAMGLKPACPVIMVAGTNGKGSTCAMLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHH
SIFRAAGFKVGAYTSPHILRYNERIAIDLKPASDERIIASFEAIDAARGDTTLTYFEFGT
HHHHHCCCEECCCCCCEEEEECCEEEEEECCCCCCCEEEHHHHHHCCCCCCEEEEEEHHH
LAAVHSFVAEKVDVIILEVGLGGRLDAVNIFEPDVSVVVSVDLDHQAILGDNREDIGFEK
HHHHHHHHHHCEEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCEECCCCCHHCCCCC
AGVYRAGKPAICVDPEPPQRLLDHAAAIGADLKLYGRDFGYTRMDNQWSFRCGDHARHAL
CCCEECCCCCEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCCCCCCC
PIPALRGAYQMINACGALAVLDAIRDKLPVGIGAIKQGLVEVEWPGRFQVLPGRPAVVLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCEEEECCCCCEEEEE
VGHNPHAVKAMAAALKQLPFAENRYAVFSMLADKDMMAVVELAKGEFDHWLVAGLDMPRG
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCC
QSGEAIASALKNAGIASVSAFDTVAQAWSAALSRAGDNDRIVVFGSFHTVAEVEAVRRHP
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCC
V
C
>Mature Secondary Structure 
TRKTLDDWLSWQESLHTAAIDMGLARVERVRGAMGLKPACPVIMVAGTNGKGSTCAMLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHH
SIFRAAGFKVGAYTSPHILRYNERIAIDLKPASDERIIASFEAIDAARGDTTLTYFEFGT
HHHHHCCCEECCCCCCEEEEECCEEEEEECCCCCCCEEEHHHHHHCCCCCCEEEEEEHHH
LAAVHSFVAEKVDVIILEVGLGGRLDAVNIFEPDVSVVVSVDLDHQAILGDNREDIGFEK
HHHHHHHHHHCEEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCEECCCCCHHCCCCC
AGVYRAGKPAICVDPEPPQRLLDHAAAIGADLKLYGRDFGYTRMDNQWSFRCGDHARHAL
CCCEECCCCCEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCCCCCCC
PIPALRGAYQMINACGALAVLDAIRDKLPVGIGAIKQGLVEVEWPGRFQVLPGRPAVVLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCEEEECCCCCEEEEE
VGHNPHAVKAMAAALKQLPFAENRYAVFSMLADKDMMAVVELAKGEFDHWLVAGLDMPRG
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCC
QSGEAIASALKNAGIASVSAFDTVAQAWSAALSRAGDNDRIVVFGSFHTVAEVEAVRRHP
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]