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The map label for this gene is rpfG [H]

Identifier: 34497952

GI number: 34497952

Start: 2687742

End: 2688878

Strand: Reverse

Name: rpfG [H]

Synonym: CV_2497

Alternate gene names: 34497952

Gene position: 2688878-2687742 (Counterclockwise)

Preceding gene: 34497953

Following gene: 34497947

Centisome position: 56.6

GC content: 63.41

Gene sequence:

>1137_bases
ATGAGCGATGCGAACGCAGCCGGGCGTCAGACCGTGCTGCTGGTGGACGACAGCCCAGAGAGCCTGACCCAGATGTACGG
TTTGCTGAAGGCGGACTATCGGACGCTGATCGCCAATGGCGGAGAACGCGCCTTGCAGATCGTCGAAGGCGATGCGCTGC
CCGACCTGGTGCTGCTGGATGTGATGATGCCGGGGCTCGGCGGGCTGGAGGTTTGCCGCCGGCTGAAGGCGGATCCGCGC
ACCGAGTCGATTCCAGTGATTTTCCTGACGGCGATGAGCGAGCTTGAGGACGAGCAGGCCGGCTTCGATGCCGGCGCCGC
GGACTACATCGTCAAGCCGGTGTCGCCACCCATCATGATGGCCAGGGTGCGCACCCATCTGCGAGCCAAGGCGGTAGCCG
ACTTTCTGGAGGACAAAGCGGCCTTCCTGCAAACCGAAGTGGAGCGACGCACGCGGGAAGTGCAGGTGATACAGGATGTC
ACCATCATGGCGCTGGCCTCGCTGGCGGAGACGCGCGACCACGAGACCGGCAACCATCTGCGCCGCACGCAGAATTATGT
CCGCGCGCTGGCCCTTGAGCTGCGCAACCATCCGAACTACCGGCCCCAGCTCAGCGACGAAACCGTGCAGCTGCTGTACA
AATCGGCGCCGTTGCACGATATAGGCAAGGTGGGGATTCCGGACCACATTCTGCTGAAGCCGGGGCCGCTGACGCCCGAA
GAGTTCGAGGTGATGAAAACGCATACCGTGCTGGGGCGCGATACCATCGCCTCAGCGGAGCGAATGCTGGACGCGCCCAG
CAGCTTTCTGCGACTGGCGCGCGAAATTGCCTATTGCCATCAGGAAAAATGGGATGGCAGCGGCTATCCCCAGGGGCTGG
CGGGGGAAGCCATCCCCTTGTCCGCCAGGCTGATGGCCGTCGCCGACGTGTACGACGCGCTGATTTCACGGCGGGTATAC
AAGCCGCCGTTTTCGCATGAAAAGGCGGTGGGGATCATACGCGACGACAGCGGCCGGCATTTTGATCCCGCGGTGGTGAA
GGCTTTCCTGCAGGTTGCCGAGCAATTCCGGGAAATCGCCCGGCTTTACTCTGATGCGGATGTTGTCGACGCCAGTGTGG
CGGCCGAGCGCGGCTGA

Upstream 100 bases:

>100_bases
ATTTCGAGCAAGCGCTGAACCGTTTCGATTTCGTTTCCGCGTTGGGCGCGCTGCGCGAGCTGGCGAGAGCTTGCGGCAAA
AAGCTGCCGGAGGACACGAG

Downstream 100 bases:

>100_bases
ACGCCGCGCGGGTTCAGGAGCGTAGCGATAAATGGGAAAAGCCGGATTGCGAGCCTAGCGGTGGTATTCGCCGGCGGCTT
CGGGCTGGTAGGTGATTTCC

Product: two component system, transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSDANAAGRQTVLLVDDSPESLTQMYGLLKADYRTLIANGGERALQIVEGDALPDLVLLDVMMPGLGGLEVCRRLKADPR
TESIPVIFLTAMSELEDEQAGFDAGAADYIVKPVSPPIMMARVRTHLRAKAVADFLEDKAAFLQTEVERRTREVQVIQDV
TIMALASLAETRDHETGNHLRRTQNYVRALALELRNHPNYRPQLSDETVQLLYKSAPLHDIGKVGIPDHILLKPGPLTPE
EFEVMKTHTVLGRDTIASAERMLDAPSSFLRLAREIAYCHQEKWDGSGYPQGLAGEAIPLSARLMAVADVYDALISRRVY
KPPFSHEKAVGIIRDDSGRHFDPAVVKAFLQVAEQFREIARLYSDADVVDASVAAERG

Sequences:

>Translated_378_residues
MSDANAAGRQTVLLVDDSPESLTQMYGLLKADYRTLIANGGERALQIVEGDALPDLVLLDVMMPGLGGLEVCRRLKADPR
TESIPVIFLTAMSELEDEQAGFDAGAADYIVKPVSPPIMMARVRTHLRAKAVADFLEDKAAFLQTEVERRTREVQVIQDV
TIMALASLAETRDHETGNHLRRTQNYVRALALELRNHPNYRPQLSDETVQLLYKSAPLHDIGKVGIPDHILLKPGPLTPE
EFEVMKTHTVLGRDTIASAERMLDAPSSFLRLAREIAYCHQEKWDGSGYPQGLAGEAIPLSARLMAVADVYDALISRRVY
KPPFSHEKAVGIIRDDSGRHFDPAVVKAFLQVAEQFREIARLYSDADVVDASVAAERG
>Mature_377_residues
SDANAAGRQTVLLVDDSPESLTQMYGLLKADYRTLIANGGERALQIVEGDALPDLVLLDVMMPGLGGLEVCRRLKADPRT
ESIPVIFLTAMSELEDEQAGFDAGAADYIVKPVSPPIMMARVRTHLRAKAVADFLEDKAAFLQTEVERRTREVQVIQDVT
IMALASLAETRDHETGNHLRRTQNYVRALALELRNHPNYRPQLSDETVQLLYKSAPLHDIGKVGIPDHILLKPGPLTPEE
FEVMKTHTVLGRDTIASAERMLDAPSSFLRLAREIAYCHQEKWDGSGYPQGLAGEAIPLSARLMAVADVYDALISRRVYK
PPFSHEKAVGIIRDDSGRHFDPAVVKAFLQVAEQFREIARLYSDADVVDASVAAERG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=118, Percent_Identity=38.9830508474576, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=34.1666666666667, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790860, Length=122, Percent_Identity=38.5245901639344, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=120, Percent_Identity=37.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=76, Percent_Identity=43.421052631579, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=124, Percent_Identity=34.6774193548387, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41776; Mature: 41645

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDANAAGRQTVLLVDDSPESLTQMYGLLKADYRTLIANGGERALQIVEGDALPDLVLLD
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCHHHHHH
VMMPGLGGLEVCRRLKADPRTESIPVIFLTAMSELEDEQAGFDAGAADYIVKPVSPPIMM
HHHCCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCHHHEECCCCCHHHH
ARVRTHLRAKAVADFLEDKAAFLQTEVERRTREVQVIQDVTIMALASLAETRDHETGNHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRTQNYVRALALELRNHPNYRPQLSDETVQLLYKSAPLHDIGKVGIPDHILLKPGPLTPE
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCHH
EFEVMKTHTVLGRDTIASAERMLDAPSSFLRLAREIAYCHQEKWDGSGYPQGLAGEAIPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SARLMAVADVYDALISRRVYKPPFSHEKAVGIIRDDSGRHFDPAVVKAFLQVAEQFREIA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RLYSDADVVDASVAAERG
HHHCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SDANAAGRQTVLLVDDSPESLTQMYGLLKADYRTLIANGGERALQIVEGDALPDLVLLD
CCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCHHHHHH
VMMPGLGGLEVCRRLKADPRTESIPVIFLTAMSELEDEQAGFDAGAADYIVKPVSPPIMM
HHHCCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCHHHEECCCCCHHHH
ARVRTHLRAKAVADFLEDKAAFLQTEVERRTREVQVIQDVTIMALASLAETRDHETGNHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRTQNYVRALALELRNHPNYRPQLSDETVQLLYKSAPLHDIGKVGIPDHILLKPGPLTPE
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCHH
EFEVMKTHTVLGRDTIASAERMLDAPSSFLRLAREIAYCHQEKWDGSGYPQGLAGEAIPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SARLMAVADVYDALISRRVYKPPFSHEKAVGIIRDDSGRHFDPAVVKAFLQVAEQFREIA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
RLYSDADVVDASVAAERG
HHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]