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The map label for this gene is ddl

Identifier: 34497927

GI number: 34497927

Start: 2659229

End: 2660329

Strand: Reverse

Name: ddl

Synonym: CV_2472

Alternate gene names: 34497927

Gene position: 2660329-2659229 (Counterclockwise)

Preceding gene: 34497928

Following gene: 34497925

Centisome position: 55.99

GC content: 63.22

Gene sequence:

>1101_bases
ATGGGCAAAATCCGGGTGGGTCTGATTTTCGGCGGACAGTCGTCCGAGCACGAGGTTTCCTTGCAATCTGCCAGGAATAT
CCTGCAAGCGATAGACGGCGAGCGTTTCGAAGTTTCGCTGATCGGTGTGGACAAGCAAGGCCGCTGGCATGCCAGCCAGG
CGTCCAATTTTCTGCTGAACGCCGACGATCCCGGCCGCATCGCCTTGCGCGAGTCCGGAGAAAACCTGGCCCTGGTGCCG
GGCGAATGTTCGGGCCAGCTGCAGACGGCGGCGAATGCGCATCCGCTGGCGCAGATCGACGTGGCCTTCCCCATCGTCCA
CGGCACGCTGGGCGAGGACGGCTCGCTGCAAGGCCTGCTGCGAATGGCCAACATCCCCTTCGTCGGCGCGGGCGTGCTGG
GTTCGGCGGTTTGCATGGACAAGGACGTGGCCAAGCGCCTGTTGCGCGATGCGGGCCTCAAAGTCGCTCCTTTCGTCAGC
CTGACTCGATCCAAGGCCGCCGGCGCCGATCTCTCCGCCATCGTCGAGCAGCTGGGATTGCCGCTGTTCGTCAAGCCGGC
CAATCAGGGCTCATCCGTAGGGGTGAGCAAAGTGAAGCGGGAGGCGGATTTGCGCGCGGCTCTGGACGAAGCTTTCCGTT
ACGATCACAAGGTCCTGGTGGAGCAGGCGGTGATAGGCCGCGAAATCGAATGCGCGGTGCTGGGCAACGAGCGCCCGCGG
GCCAGCGGCTGCGGCGAGATCGTGTTGAGCGATGAGTTCTACGCCTACGACACCAAGTATCTGAATGAGGACGGCGCCCG
CGTCGCAGTGCCGGCCGACATCCCGGACGAGGCCTGCCAGCGCATCCGCGGCATCGCCATTGAGGCTTTCCAGGCGCTGG
AGTGCAGCGGCATGGCGCGCGTGGATGTGTTCCTGACGCCGGATGGCGAGGTGGTGATCAATGAACTGAACACGCTGCCG
GGTTTCACCAATATCAGCATGTACCCCAAGCTGTGGCAGGCGGCTGGGATGTCGTATCGGGAATTGATCACCGCGCTGAT
AGAGTTGGCGCTGGAGAAAGGCCGGATGGATGAAGCGTTGAGCCGCAGTTGCCAGTACTAG

Upstream 100 bases:

>100_bases
ACGCCGACGAGGCGCCTGGCGAAGAGTAGGCGCAGGCCGAGTGGCGGCATCGAAAATACATTATCATGCCGCGTTCCTGT
TTTTCATATTGAAGGCAGTC

Downstream 100 bases:

>100_bases
CCGCGCCCTTTGCGCGCTGGTGCGGTCTGCGTCGGCGCGCCTTGCCTCAGCCGTCAAACTGAATTTCGTTAGCCACTCTC
AACGCCTGACAGGCCCGCCT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MGKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLNADDPGRIALRESGENLALVP
GECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLLRMANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVS
LTRSKAAGADLSAIVEQLGLPLFVKPANQGSSVGVSKVKREADLRAALDEAFRYDHKVLVEQAVIGREIECAVLGNERPR
ASGCGEIVLSDEFYAYDTKYLNEDGARVAVPADIPDEACQRIRGIAIEAFQALECSGMARVDVFLTPDGEVVINELNTLP
GFTNISMYPKLWQAAGMSYRELITALIELALEKGRMDEALSRSCQY

Sequences:

>Translated_366_residues
MGKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLNADDPGRIALRESGENLALVP
GECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLLRMANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVS
LTRSKAAGADLSAIVEQLGLPLFVKPANQGSSVGVSKVKREADLRAALDEAFRYDHKVLVEQAVIGREIECAVLGNERPR
ASGCGEIVLSDEFYAYDTKYLNEDGARVAVPADIPDEACQRIRGIAIEAFQALECSGMARVDVFLTPDGEVVINELNTLP
GFTNISMYPKLWQAAGMSYRELITALIELALEKGRMDEALSRSCQY
>Mature_365_residues
GKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLNADDPGRIALRESGENLALVPG
ECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLLRMANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVSL
TRSKAAGADLSAIVEQLGLPLFVKPANQGSSVGVSKVKREADLRAALDEAFRYDHKVLVEQAVIGREIECAVLGNERPRA
SGCGEIVLSDEFYAYDTKYLNEDGARVAVPADIPDEACQRIRGIAIEAFQALECSGMARVDVFLTPDGEVVINELNTLPG
FTNISMYPKLWQAAGMSYRELITALIELALEKGRMDEALSRSCQY

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=363, Percent_Identity=66.3911845730028, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1786280, Length=256, Percent_Identity=40.234375, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLA_CHRVO (Q7NV72)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902142.1
- ProteinModelPortal:   Q7NV72
- SMR:   Q7NV72
- GeneID:   2548937
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2472
- NMPDR:   fig|243365.1.peg.2472
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- PhylomeDB:   Q7NV72
- ProtClustDB:   PRK01966
- BioCyc:   CVIO243365:CV_2472-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39309; Mature: 39178

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCEEEEEEEEECCCCCEECCCCCCEEEE
ADDPGRIALRESGENLALVPGECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLL
CCCCCEEEEEECCCEEEEECCCCCCCEECCCCCCCCEEEEEEEEEEECCCCCCCCHHHHH
RMANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVSLTRSKAAGADLSAIVEQLGL
HHHCCCEEECHHHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCHHHHHHHHCC
PLFVKPANQGSSVGVSKVKREADLRAALDEAFRYDHKVLVEQAVIGREIECAVLGNERPR
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
ASGCGEIVLSDEFYAYDTKYLNEDGARVAVPADIPDEACQRIRGIAIEAFQALECSGMAR
CCCCCEEEECCCEEEECHHEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VDVFLTPDGEVVINELNTLPGFTNISMYPKLWQAAGMSYRELITALIELALEKGRMDEAL
EEEEECCCCCEEEEHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHH
SRSCQY
HHHCCC
>Mature Secondary Structure 
GKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLN
CCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCEEEEEEEEECCCCCEECCCCCCEEEE
ADDPGRIALRESGENLALVPGECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLL
CCCCCEEEEEECCCEEEEECCCCCCCEECCCCCCCCEEEEEEEEEEECCCCCCCCHHHHH
RMANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVSLTRSKAAGADLSAIVEQLGL
HHHCCCEEECHHHHHHHHCCHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCHHHHHHHHCC
PLFVKPANQGSSVGVSKVKREADLRAALDEAFRYDHKVLVEQAVIGREIECAVLGNERPR
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
ASGCGEIVLSDEFYAYDTKYLNEDGARVAVPADIPDEACQRIRGIAIEAFQALECSGMAR
CCCCCEEEECCCEEEECHHEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VDVFLTPDGEVVINELNTLPGFTNISMYPKLWQAAGMSYRELITALIELALEKGRMDEAL
EEEEECCCCCEEEEHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHH
SRSCQY
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782