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The map label for this gene is gstA [H]

Identifier: 34497879

GI number: 34497879

Start: 2607863

End: 2608564

Strand: Reverse

Name: gstA [H]

Synonym: CV_2424

Alternate gene names: 34497879

Gene position: 2608564-2607863 (Counterclockwise)

Preceding gene: 34497882

Following gene: 34497878

Centisome position: 54.9

GC content: 65.53

Gene sequence:

>702_bases
ATGATAGACCTTCATTACTGGACCACGCCCAACGGCCACAAGATCACCCTGTTCCTCGAGGAGGCCGGCCTGCCTTACCG
CATCGTGCCGGTGAACATCTCCGCCGGCGATCAGTTCCAGCCCGATTTCCTGCGCATCGCGCCCAATAATCGCATTCCGG
CCATCGTCGACCATGCGCCGCAAGACGGCGGCGAACCGATCAGCCTGTTCGAATCCGGAGCCATCTTGCTGTATCTGGCC
GACAAGACCGGCCGCTTCATTCCGCAAGACCTGCGCGGCCGCAACGAGGCGCTGCAGTGGCTGTTCTGGCAGATGGGCGG
CCTGGGGCCGATGGCGGGCCAGAACCACCATTTCTCCCAGTACGCGCCGGAAAAGCTGCCATACGCGATCGACCGCTACG
TGCGCGAGACCGCCCGCCTCTACCGCGTGCTGGACACGCATTTGTCCGACGGCCGCGACTTCATCGCCGGCGATTACTCG
ATCGCCGACATGGCCTGCTACCCGTGGATCGTGCCGCACCAGCGCCAGCAGCAGAACCTGGACGACTTCCCTTCGCTCAA
GCGCTGGTTCGAGCGCATCGCCGAGCGTCCCGCGGTGAAGCGCGCCTATGCGCTGGCGGAAAGCGTCAATACCGCGCCGT
CGGTCACCGAGGCCTCCCGCGCCATCCTGTTCGGCCAGGACGGCAAGCCGCGCGGCGGCTGA

Upstream 100 bases:

>100_bases
CTTGATTGGGTTTCGCGCAATCATACCGCGCGCGGATGCTCTTTCAAAACGCGGGCATGCCCCCATGTCATTCTTTCCAT
TCCCATCCAGGAGGCGAACG

Downstream 100 bases:

>100_bases
TCCCGCTCCTTTCGCTTCCTTCCATGCGCGGCGGCCATCGGCCGCCGCTCTTTCAAATCTGATCGCTCCGCCGGATTTTG
ATCGGCTTTTCATCAGGAAC

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MIDLHYWTTPNGHKITLFLEEAGLPYRIVPVNISAGDQFQPDFLRIAPNNRIPAIVDHAPQDGGEPISLFESGAILLYLA
DKTGRFIPQDLRGRNEALQWLFWQMGGLGPMAGQNHHFSQYAPEKLPYAIDRYVRETARLYRVLDTHLSDGRDFIAGDYS
IADMACYPWIVPHQRQQQNLDDFPSLKRWFERIAERPAVKRAYALAESVNTAPSVTEASRAILFGQDGKPRGG

Sequences:

>Translated_233_residues
MIDLHYWTTPNGHKITLFLEEAGLPYRIVPVNISAGDQFQPDFLRIAPNNRIPAIVDHAPQDGGEPISLFESGAILLYLA
DKTGRFIPQDLRGRNEALQWLFWQMGGLGPMAGQNHHFSQYAPEKLPYAIDRYVRETARLYRVLDTHLSDGRDFIAGDYS
IADMACYPWIVPHQRQQQNLDDFPSLKRWFERIAERPAVKRAYALAESVNTAPSVTEASRAILFGQDGKPRGG
>Mature_233_residues
MIDLHYWTTPNGHKITLFLEEAGLPYRIVPVNISAGDQFQPDFLRIAPNNRIPAIVDHAPQDGGEPISLFESGAILLYLA
DKTGRFIPQDLRGRNEALQWLFWQMGGLGPMAGQNHHFSQYAPEKLPYAIDRYVRETARLYRVLDTHLSDGRDFIAGDYS
IADMACYPWIVPHQRQQQNLDDFPSLKRWFERIAERPAVKRAYALAESVNTAPSVTEASRAILFGQDGKPRGG

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=207, Percent_Identity=59.4202898550725, Blast_Score=260, Evalue=6e-71,
Organism=Escherichia coli, GI87082195, Length=215, Percent_Identity=42.3255813953488, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI87081789, Length=206, Percent_Identity=29.126213592233, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571670, Length=178, Percent_Identity=27.5280898876405, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864592, Length=197, Percent_Identity=24.8730964467005, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26377; Mature: 26377

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLHYWTTPNGHKITLFLEEAGLPYRIVPVNISAGDQFQPDFLRIAPNNRIPAIVDHAP
CEEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
QDGGEPISLFESGAILLYLADKTGRFIPQDLRGRNEALQWLFWQMGGLGPMAGQNHHFSQ
CCCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKLPYAIDRYVRETARLYRVLDTHLSDGRDFIAGDYSIADMACYPWIVPHQRQQQNL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCHHHCCC
DDFPSLKRWFERIAERPAVKRAYALAESVNTAPSVTEASRAILFGQDGKPRGG
CCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHCEEEEECCCCCCCCC
>Mature Secondary Structure
MIDLHYWTTPNGHKITLFLEEAGLPYRIVPVNISAGDQFQPDFLRIAPNNRIPAIVDHAP
CEEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
QDGGEPISLFESGAILLYLADKTGRFIPQDLRGRNEALQWLFWQMGGLGPMAGQNHHFSQ
CCCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKLPYAIDRYVRETARLYRVLDTHLSDGRDFIAGDYSIADMACYPWIVPHQRQQQNL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCHHHCCC
DDFPSLKRWFERIAERPAVKRAYALAESVNTAPSVTEASRAILFGQDGKPRGG
CCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]