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The map label for this gene is priA [H]

Identifier: 33863539

GI number: 33863539

Start: 1357251

End: 1359557

Strand: Reverse

Name: priA [H]

Synonym: PMT1271

Alternate gene names: 33863539

Gene position: 1359557-1357251 (Counterclockwise)

Preceding gene: 33863542

Following gene: 33863534

Centisome position: 56.39

GC content: 57.0

Gene sequence:

>2307_bases
GTGACGCCTGCAAGTGCTGATCCGGCTGAACTGCCCCACTGCCCTTCCGAACGCTGCGCAGAGGTGGACGTCTGGGTGGA
GGCAGGCCGTGAAGGGCGGTGTTTCACCTATGTGGACAGCAGACGGTTAGGGGTGGATTTAGGGGATCTCGTCGTCGTTC
GCTTGCGGGGACGAAGGATGCACGGCCTCGTGATGGATCGGCGGATCTCGTCTCCTGTTGATCGTGGCCAGGACTCTGGG
AGTGAAGCACCGCCGCGTCACTTAGAGGCCATCGAGGCTCTTGTGCAGTCTGCGGCGGTAGATCCTCTCTGGTTTGGCTG
GATTGAAGCGATGGCTGTTCATTGCCATATCAGTTCATTTCGCATGCTTAAAGCAGCTTTGCCGCCTGGTTGGCTCGGTC
AGCGGCAGTCTCATCAGGCGGAGCCCCGTCGACTCTGGTGGATTCAACTCGAATCTTCTGCGATCAATCCCCAGAATCTT
CCTCAGCGTCAGGCTGATTTGCAGGCGGCCTTGGCTGCAGGGGGTGGTGGTGCATGGCAGAGGGATCTTCAGGCTGCTGG
CTTCGGGTCTGGTCTTGTCAATGGCCTCATCAAGCGAGGTCTCATTCGTAGGGAAAAGCGTCAACCTACTGATGCCTCCA
ACGGGCTTTCCTGCTCAGATGCTTGTGATCAGGATTTGGAGGTGCCTCAGTCTCTGACTGTTGAACAGCAGGAGGTTGTG
GAGGCTTTCCAATCCCAGCCTCTTGGCACTGGGATGCTGCTTTGGGGGGTGACAGGCTCGGGCAAGACAGAGGTTTACTT
GCAGCTTGCGGCAAGAGAACTGCAAGCCGGTCGTCATTGCTTGATTCTCACGCCGGAGATTGGTTTGATCCCTCAGTTGG
TCGATCGTTTCCGTCGCCGTTTCGGAACCAAGGTTCTGGAATATCACAGTGGCTGTTCCGATCGTGAACGGGTGAGCACC
TGGAGGCAGGGGCTTACTGCTGCCACTCCATTGGTGGTGGTTGGTACTCGTTCAGCAGTCTTTTTGCCATTGGCACCATT
GGGTTTGATCGTGCTTGATGAGGAACACGACAGTTCTTACAAGCAGGAGTCACCCATGCCTTGCTACCACGCCCGTGATA
TGGCCATGGATAGGGCCAGGCGAACTGGCGCCAGGGTGGTGCTGGGAAGCGCTACGCCTTCGCTTGTCAGCTGGAAGAAC
CTCGCTCCTCAAGGCCAGCTTGCCTTGGCTCGACTGACCCGTCGCATTTCCGATCAGCCCTTACCGCCTGTGCATGTGGT
TGATATGCGACAGGAATTGGCCGATGGCCATCGTCGCTTGATCAGCCGTCCATTGATGGAACGTCTTTCGGCATTGCCTG
AGGCCGGTGAACAGGCGGTGGTGTTGGTGCCACGACGCGGCTACAGCAGCTTTTTGAGTTGTCGTAGCTGTGGAGAGGTC
GTGCAGTGTCCTAACTGCGACGTGGCGCTTACGGTGCATCGCAGTCGTCAAGGGCACCAGTGGTTGCGTTGCCATTGGTG
TGACCATCGTGCTGAGGTTATGTCCAGTTGCCATAAATGTGGATCGAAGGCTTTCAAACCCTTTGGTGCAGGCACGCAGC
GCGTGATGGAGCATCTCGTTGAGGAGCTCCAGGGTCTGAGGTTGTTGCGTTTCGATCGTGACAGCACCGCTGGGCGTGAT
GGGCATCGCCGTTTACTGGAGCAATTTGCAGCAGGGGAGGCAGATGTGCTGGTTGGCACCCAGATGTTGGCCAAGGGTAT
GGATTTGCCTCGGGTCACTCTTGCGGCGGTGTTGGCTGCAGATGGTTTGCTGCATCGCCCTGATTTACAAGCTGGAGAGC
AGAGCTTGCAACTGTTGATGCAATTGGCTGGTCGTGCAGGACGTGGCGAGCGGCCAGGTCATGTGCTTGTTCAGACCTAT
TGCCCTGATCATCCTGTGATTCACCATTTGGTGGATGGTCGCTATGGCGAGTTTCTGAAAGAAGAGGCCTGTCTTCGTCA
TGAGGCTGGTTTGGTGCCTTATAGCCGGGCCTGCTTGTTGCGTTTGTCAGGTGATTCAGCTGCCGTGACAGCCACAGCTG
CTGCGGTCCTCGCTGAACAGATCAAACCCCTTTGTGAGGCTCAGGGTTGGTGTCTTGTAGGACCAGCGCCAGCTCCGATT
GCACGGGTCGCTGGTCGAAGTCGTTGGCAACTGCTATTGCATGGTCCGGAGGAGAGTCGTTTGCCGCTGCCATCAGGATC
GACTCTTTGGAATGGATTGCCCCGTGGGGTTTCCTTAGCGGTGGACCCAGATCCAATTCAGCTTTGA

Upstream 100 bases:

>100_bases
TATAGAGGCTCGAATTTCGTGTCTTCCCACTGGACGGAACTCTGCTTGGTTCCGACGACAGCCTTTGGACTGTCCAACAT
TGACATTCTAAAAAAAACTT

Downstream 100 bases:

>100_bases
TTAGGCCATCATCTTCAATGGTTGATGTTGTTGGAGGTCCGTTCGAGGGGCATTTTGAGCACGCCGAAGCCGAGTCTGAG
CTGAACGTTTTGCAGGATCA

Product: primosomal protein N' (replication factor Y)

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 768; Mature: 767

Protein sequence:

>768_residues
MTPASADPAELPHCPSERCAEVDVWVEAGREGRCFTYVDSRRLGVDLGDLVVVRLRGRRMHGLVMDRRISSPVDRGQDSG
SEAPPRHLEAIEALVQSAAVDPLWFGWIEAMAVHCHISSFRMLKAALPPGWLGQRQSHQAEPRRLWWIQLESSAINPQNL
PQRQADLQAALAAGGGGAWQRDLQAAGFGSGLVNGLIKRGLIRREKRQPTDASNGLSCSDACDQDLEVPQSLTVEQQEVV
EAFQSQPLGTGMLLWGVTGSGKTEVYLQLAARELQAGRHCLILTPEIGLIPQLVDRFRRRFGTKVLEYHSGCSDRERVST
WRQGLTAATPLVVVGTRSAVFLPLAPLGLIVLDEEHDSSYKQESPMPCYHARDMAMDRARRTGARVVLGSATPSLVSWKN
LAPQGQLALARLTRRISDQPLPPVHVVDMRQELADGHRRLISRPLMERLSALPEAGEQAVVLVPRRGYSSFLSCRSCGEV
VQCPNCDVALTVHRSRQGHQWLRCHWCDHRAEVMSSCHKCGSKAFKPFGAGTQRVMEHLVEELQGLRLLRFDRDSTAGRD
GHRRLLEQFAAGEADVLVGTQMLAKGMDLPRVTLAAVLAADGLLHRPDLQAGEQSLQLLMQLAGRAGRGERPGHVLVQTY
CPDHPVIHHLVDGRYGEFLKEEACLRHEAGLVPYSRACLLRLSGDSAAVTATAAAVLAEQIKPLCEAQGWCLVGPAPAPI
ARVAGRSRWQLLLHGPEESRLPLPSGSTLWNGLPRGVSLAVDPDPIQL

Sequences:

>Translated_768_residues
MTPASADPAELPHCPSERCAEVDVWVEAGREGRCFTYVDSRRLGVDLGDLVVVRLRGRRMHGLVMDRRISSPVDRGQDSG
SEAPPRHLEAIEALVQSAAVDPLWFGWIEAMAVHCHISSFRMLKAALPPGWLGQRQSHQAEPRRLWWIQLESSAINPQNL
PQRQADLQAALAAGGGGAWQRDLQAAGFGSGLVNGLIKRGLIRREKRQPTDASNGLSCSDACDQDLEVPQSLTVEQQEVV
EAFQSQPLGTGMLLWGVTGSGKTEVYLQLAARELQAGRHCLILTPEIGLIPQLVDRFRRRFGTKVLEYHSGCSDRERVST
WRQGLTAATPLVVVGTRSAVFLPLAPLGLIVLDEEHDSSYKQESPMPCYHARDMAMDRARRTGARVVLGSATPSLVSWKN
LAPQGQLALARLTRRISDQPLPPVHVVDMRQELADGHRRLISRPLMERLSALPEAGEQAVVLVPRRGYSSFLSCRSCGEV
VQCPNCDVALTVHRSRQGHQWLRCHWCDHRAEVMSSCHKCGSKAFKPFGAGTQRVMEHLVEELQGLRLLRFDRDSTAGRD
GHRRLLEQFAAGEADVLVGTQMLAKGMDLPRVTLAAVLAADGLLHRPDLQAGEQSLQLLMQLAGRAGRGERPGHVLVQTY
CPDHPVIHHLVDGRYGEFLKEEACLRHEAGLVPYSRACLLRLSGDSAAVTATAAAVLAEQIKPLCEAQGWCLVGPAPAPI
ARVAGRSRWQLLLHGPEESRLPLPSGSTLWNGLPRGVSLAVDPDPIQL
>Mature_767_residues
TPASADPAELPHCPSERCAEVDVWVEAGREGRCFTYVDSRRLGVDLGDLVVVRLRGRRMHGLVMDRRISSPVDRGQDSGS
EAPPRHLEAIEALVQSAAVDPLWFGWIEAMAVHCHISSFRMLKAALPPGWLGQRQSHQAEPRRLWWIQLESSAINPQNLP
QRQADLQAALAAGGGGAWQRDLQAAGFGSGLVNGLIKRGLIRREKRQPTDASNGLSCSDACDQDLEVPQSLTVEQQEVVE
AFQSQPLGTGMLLWGVTGSGKTEVYLQLAARELQAGRHCLILTPEIGLIPQLVDRFRRRFGTKVLEYHSGCSDRERVSTW
RQGLTAATPLVVVGTRSAVFLPLAPLGLIVLDEEHDSSYKQESPMPCYHARDMAMDRARRTGARVVLGSATPSLVSWKNL
APQGQLALARLTRRISDQPLPPVHVVDMRQELADGHRRLISRPLMERLSALPEAGEQAVVLVPRRGYSSFLSCRSCGEVV
QCPNCDVALTVHRSRQGHQWLRCHWCDHRAEVMSSCHKCGSKAFKPFGAGTQRVMEHLVEELQGLRLLRFDRDSTAGRDG
HRRLLEQFAAGEADVLVGTQMLAKGMDLPRVTLAAVLAADGLLHRPDLQAGEQSLQLLMQLAGRAGRGERPGHVLVQTYC
PDHPVIHHLVDGRYGEFLKEEACLRHEAGLVPYSRACLLRLSGDSAAVTATAAAVLAEQIKPLCEAQGWCLVGPAPAPIA
RVAGRSRWQLLLHGPEESRLPLPSGSTLWNGLPRGVSLAVDPDPIQL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=702, Percent_Identity=34.3304843304843, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84204; Mature: 84072

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00028 ZINC_FINGER_C2H2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASADPAELPHCPSERCAEVDVWVEAGREGRCFTYVDSRRLGVDLGDLVVVRLRGRRM
CCCCCCCCCCCCCCCHHHHCEEHHHEECCCCCCEEEEECCCCCCCCHHHEEEEEECCCCH
HGLVMDRRISSPVDRGQDSGSEAPPRHLEAIEALVQSAAVDPLWFGWIEAMAVHCHISSF
HHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RMLKAALPPGWLGQRQSHQAEPRRLWWIQLESSAINPQNLPQRQADLQAALAAGGGGAWQ
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHH
RDLQAAGFGSGLVNGLIKRGLIRREKRQPTDASNGLSCSDACDQDLEVPQSLTVEQQEVV
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHH
EAFQSQPLGTGMLLWGVTGSGKTEVYLQLAARELQAGRHCLILTPEIGLIPQLVDRFRRR
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
FGTKVLEYHSGCSDRERVSTWRQGLTAATPLVVVGTRSAVFLPLAPLGLIVLDEEHDSSY
HCHHHHHHHCCCCHHHHHHHHHHCCHHCCCEEEEECCCEEEEEECCCCEEEEECCCCCCC
KQESPMPCYHARDMAMDRARRTGARVVLGSATPSLVSWKNLAPQGQLALARLTRRISDQP
CCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCC
LPPVHVVDMRQELADGHRRLISRPLMERLSALPEAGEQAVVLVPRRGYSSFLSCRSCGEV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCHH
VQCPNCDVALTVHRSRQGHQWLRCHWCDHRAEVMSSCHKCGSKAFKPFGAGTQRVMEHLV
HCCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EELQGLRLLRFDRDSTAGRDGHRRLLEQFAAGEADVLVGTQMLAKGMDLPRVTLAAVLAA
HHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
DGLLHRPDLQAGEQSLQLLMQLAGRAGRGERPGHVLVQTYCPDHPVIHHLVDGRYGEFLK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHH
EEACLRHEAGLVPYSRACLLRLSGDSAAVTATAAAVLAEQIKPLCEAQGWCLVGPAPAPI
HHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
ARVAGRSRWQLLLHGPEESRLPLPSGSTLWNGLPRGVSLAVDPDPIQL
HHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TPASADPAELPHCPSERCAEVDVWVEAGREGRCFTYVDSRRLGVDLGDLVVVRLRGRRM
CCCCCCCCCCCCCCHHHHCEEHHHEECCCCCCEEEEECCCCCCCCHHHEEEEEECCCCH
HGLVMDRRISSPVDRGQDSGSEAPPRHLEAIEALVQSAAVDPLWFGWIEAMAVHCHISSF
HHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RMLKAALPPGWLGQRQSHQAEPRRLWWIQLESSAINPQNLPQRQADLQAALAAGGGGAWQ
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHH
RDLQAAGFGSGLVNGLIKRGLIRREKRQPTDASNGLSCSDACDQDLEVPQSLTVEQQEVV
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHH
EAFQSQPLGTGMLLWGVTGSGKTEVYLQLAARELQAGRHCLILTPEIGLIPQLVDRFRRR
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
FGTKVLEYHSGCSDRERVSTWRQGLTAATPLVVVGTRSAVFLPLAPLGLIVLDEEHDSSY
HCHHHHHHHCCCCHHHHHHHHHHCCHHCCCEEEEECCCEEEEEECCCCEEEEECCCCCCC
KQESPMPCYHARDMAMDRARRTGARVVLGSATPSLVSWKNLAPQGQLALARLTRRISDQP
CCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCC
LPPVHVVDMRQELADGHRRLISRPLMERLSALPEAGEQAVVLVPRRGYSSFLSCRSCGEV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCHH
VQCPNCDVALTVHRSRQGHQWLRCHWCDHRAEVMSSCHKCGSKAFKPFGAGTQRVMEHLV
HCCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EELQGLRLLRFDRDSTAGRDGHRRLLEQFAAGEADVLVGTQMLAKGMDLPRVTLAAVLAA
HHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
DGLLHRPDLQAGEQSLQLLMQLAGRAGRGERPGHVLVQTYCPDHPVIHHLVDGRYGEFLK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHH
EEACLRHEAGLVPYSRACLLRLSGDSAAVTATAAAVLAEQIKPLCEAQGWCLVGPAPAPI
HHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
ARVAGRSRWQLLLHGPEESRLPLPSGSTLWNGLPRGVSLAVDPDPIQL
HHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]