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The map label for this gene is leuA [H]

Identifier: 33863392

GI number: 33863392

Start: 1211182

End: 1212804

Strand: Reverse

Name: leuA [H]

Synonym: PMT1121

Alternate gene names: 33863392

Gene position: 1212804-1211182 (Counterclockwise)

Preceding gene: 33863394

Following gene: 33863391

Centisome position: 50.31

GC content: 53.05

Gene sequence:

>1623_bases
ATGGCCAAGGATCCAGGCCGCGTTTTGATTTTTGACACCACCCTCAGGGATGGAGAGCAGTCCCCTGGGGCAAGTCTCAA
TCTTGAGGAAAAGCTGGCTATTGCCCAGCAACTCGCTCGTTTGGGTGTGGATGTGATAGAAGCTGGCTTTCCTTTTGCCA
GCCAGGGCGATTTTGCTGCCGTGCAACGTATTGCCCAGCAGGTTGGTGGGGATGAAGGGCCAATCATTTGTGGCTTGGCT
AGGGCTTCGCGTGCTGATATCAAGGCTTGTGCTGATGCCGTTGCCCCAGCACCTCGACGACGAATTCACACATTCATTGC
CACGAGTGATATTCACTTGGAGCACAAGCTGCGTAAATCTCGGGCGGAGGTTCTGGCGATTGTTCCAGAGATGGTTGCCT
ACGCCCGCTCACTTGTTGATGATGTTGAGTTCTCTTGTGAGGACGCCGCTAGAAGCGATCCTGAGTTTCTTTATGAGGTG
ATCGAGGCTGCGATTGCTGCGGGGGCGGGCACCATCAATATCCCCGACACAGTTGGTTTTACAACTCCCTCTGAGTTCGG
GGCGTTGATTGCTGGTATCGATTGCCATGTTCCAAACATGAATGAGGCGGTTATTTCTGTTCATGGTCACAACGACCTCG
GACTGGCTGTAGCCAATTTTTTAGAAGCTGTGAAGAGTGGAGCCCGGCAGTTTGAATGCACAATCAACGGCATTGGAGAG
CGAGCAGGGAATGCTGCGCTTGAGGAGTTGGTGATGGCTTTGTATGTGAGGCGGCGTTATTTCAATCCCTTCTTTGGGCG
TGAACCTGATAGCCCTACTCCTCTAACAGCCGTTCGCACGGAGGAAATCACCAAGACCTCTCGATTGGTCTCAAATCTCA
CCGGCATGGTGGTGCAGCCCAATAAGGCGATTGTAGGCAGCAATGCTTTCGCCCATGAGTCTGGTATTCATCAAGATGGT
GTGCTTAAAAATCGTCTGACTTATGAAATTGTTGATGCCCGTACGGTTGGGCTGAGCGACAATCGGATCTCGCTTGGCAA
GCTAAGTGGCCGCAGTGCCGTCCGAGCTCGCCTTGAGGAGCTCGGTTACGACTTGACTCGGGAGGATCTTGACGAGGCCT
TTGCACGATTTAAGGATCTGGCCGATCGCAAGCGCGATATAACCGATCGAGATCTTGAGGCCATTGTCAGCGAACAGGTT
CAGCAGTCTGAAGCTCGTTTTCAGTTACGACTGGTGCAAGTCAGTTGCGGTAGCAGTCTTAGGCCTACGGCTACTGTCAT
TCTTGCGCAAGAGGATGGACAGGAGCAGACTGCCGCTGCAGTGGGCACCGGTCCAGTAGATGCTGTCTGTCGTGCATTGA
ATGCTCTAGCTGGCGAACCTAATGAGCTGATCGAGTTCTCGGTGAAGTCGGTAACGGAAGGGATTGATGCGATGGGTGAA
GTCACGATTCGGCTACGTCGGGATGGGCAGCTTTTCTCAGGTCATTCCGCTGACACTGATGTGGTTGTTGCTGCAGCTCA
AGCATTTGTGAATGCGCTTAATCGGCTGGTGGCAGGTTGCGGTCGCCAGTCTCTTCATCCCCAACATGATGCTGTGCTAG
CTGATCTACGGTCAGGCATCTGA

Upstream 100 bases:

>100_bases
CCGACCTTGGTTTGCTGAGTTGTCGCTTCGCAGTAGGCAGTCCCCCCTTAGACTTGTTTAACTCATAGTCATTTCGACTA
ACTCCCTGAAGGGTGCTGTC

Downstream 100 bases:

>100_bases
ACAATGGGGCGTTTTCCCTATGTGGAGATGAGGCGTAAGAGCTTGGCTTTGCCACTGCTGAAAGGTCTTCAGTTAGGGGT
CCTTATCGTGTTGGCGCTTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFAAVQRIAQQVGGDEGPIICGLA
RASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKSRAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEV
IEAAIAAGAGTINIPDTVGFTTPSEFGALIAGIDCHVPNMNEAVISVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
RAGNAALEELVMALYVRRRYFNPFFGREPDSPTPLTAVRTEEITKTSRLVSNLTGMVVQPNKAIVGSNAFAHESGIHQDG
VLKNRLTYEIVDARTVGLSDNRISLGKLSGRSAVRARLEELGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQV
QQSEARFQLRLVQVSCGSSLRPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADLRSGI

Sequences:

>Translated_540_residues
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFAAVQRIAQQVGGDEGPIICGLA
RASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKSRAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEV
IEAAIAAGAGTINIPDTVGFTTPSEFGALIAGIDCHVPNMNEAVISVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
RAGNAALEELVMALYVRRRYFNPFFGREPDSPTPLTAVRTEEITKTSRLVSNLTGMVVQPNKAIVGSNAFAHESGIHQDG
VLKNRLTYEIVDARTVGLSDNRISLGKLSGRSAVRARLEELGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQV
QQSEARFQLRLVQVSCGSSLRPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADLRSGI
>Mature_539_residues
AKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFAAVQRIAQQVGGDEGPIICGLAR
ASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKSRAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVI
EAAIAAGAGTINIPDTVGFTTPSEFGALIAGIDCHVPNMNEAVISVHGHNDLGLAVANFLEAVKSGARQFECTINGIGER
AGNAALEELVMALYVRRRYFNPFFGREPDSPTPLTAVRTEEITKTSRLVSNLTGMVVQPNKAIVGSNAFAHESGIHQDGV
LKNRLTYEIVDARTVGLSDNRISLGKLSGRSAVRARLEELGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQ
QSEARFQLRLVQVSCGSSLRPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGEV
TIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADLRSGI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=512, Percent_Identity=48.828125, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320019, Length=389, Percent_Identity=28.7917737789203, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=393, Percent_Identity=29.0076335877863, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=501, Percent_Identity=26.7465069860279, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=500, Percent_Identity=25.2, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57971; Mature: 57840

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFAA
CCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VQRIAQQVGGDEGPIICGLARASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVIEAAIAAGAGTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCC
TTPSEFGALIAGIDCHVPNMNEAVISVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
CCHHHHHHHHCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
RAGNAALEELVMALYVRRRYFNPFFGREPDSPTPLTAVRTEEITKTSRLVSNLTGMVVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEECC
NKAIVGSNAFAHESGIHQDGVLKNRLTYEIVDARTVGLSDNRISLGKLSGRSAVRARLEE
CCEEECCCCHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQQSEARFQLRLVQVSCGSSL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCC
RPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
CCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADLRSGI
EEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASQGDFAA
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VQRIAQQVGGDEGPIICGLARASRADIKACADAVAPAPRRRIHTFIATSDIHLEHKLRKS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RAEVLAIVPEMVAYARSLVDDVEFSCEDAARSDPEFLYEVIEAAIAAGAGTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCC
TTPSEFGALIAGIDCHVPNMNEAVISVHGHNDLGLAVANFLEAVKSGARQFECTINGIGE
CCHHHHHHHHCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
RAGNAALEELVMALYVRRRYFNPFFGREPDSPTPLTAVRTEEITKTSRLVSNLTGMVVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEECC
NKAIVGSNAFAHESGIHQDGVLKNRLTYEIVDARTVGLSDNRISLGKLSGRSAVRARLEE
CCEEECCCCHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYDLTREDLDEAFARFKDLADRKRDITDRDLEAIVSEQVQQSEARFQLRLVQVSCGSSL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCC
RPTATVILAQEDGQEQTAAAVGTGPVDAVCRALNALAGEPNELIEFSVKSVTEGIDAMGE
CCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCE
VTIRLRRDGQLFSGHSADTDVVVAAAQAFVNALNRLVAGCGRQSLHPQHDAVLADLRSGI
EEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA