Click here to switch to the map view.

The map label for this gene is phnE [H]

Identifier: 33863054

GI number: 33863054

Start: 856738

End: 858303

Strand: Direct

Name: phnE [H]

Synonym: PMT0782

Alternate gene names: 33863054

Gene position: 856738-858303 (Clockwise)

Preceding gene: 33863053

Following gene: 33863055

Centisome position: 35.54

GC content: 47.25

Gene sequence:

>1566_bases
ATGAGTCGCCTCAGTCCAACTCCTCCAATCCTGAGCCTTTTGCCAGCACTGGCTTTATTGCCTGTACTTATTTCTATACT
CATAAGTTTCCACAATGGTGGAGTCACTCTCCTATGGGAGTTTATTTGCGCTGCCGTTCATCCATCTATTCAAGCAGAGG
TCGTTCAAAGTGCATTTAGAGGAATACAGATCACTTTGGCCACAGCTCTTCTTAGCTGGTCAATAAGTTCAATAGTTGGA
ATTTGTCTTGGCATAGCCAGCGCCAGGGTGGTATGGGAGACCTTTTTAGGGACAAACTGGCCAGCGATTGTGATTCGCAG
ACTTTTGGCAATCCCGCGCGCAATTCATGAACTCCTCTGGGGTTTGTTGTTACTTCAAATGGTTGGACTTAGCCCTTGGG
TAGCAATTGTTGCAATCGTCATTCCTTACTCCTCACTTGTTGCTCGAGTCGTAAGTGATCAACTTGACACCCAGGATCGT
CGAGCCCTAAGGGGCTTAAAACAGGCTGGGGCGGGCTCCTCTTCCGCCTTAATTACAGCTCTTGGCCCTGCGATGGGGCC
TGTCTTATTGAGTTATGTCGGATATCGACTTGAATGTGCATTAAGAGGTGCAACCGTGTTGGGGGTTTTTGGATTGGGTG
GTATTGGCACTGACCTTCAACTGGCACTTCAATCTCTTCAATTCCATGACGTATGGACAGCTTTATGGTTACTAGCAGCA
GTAATGGTTAGTCTTGAGCAGATATTAAGTTTGACACGTAGATATTTCAAATTATCGAAGAATGGGATTTGGCAAGTTGC
CAGTATATTGATTGTCTTATTGATTCTATGCGCACTAAGTATTCCCTGGCTAAATGCAGTTGATCTTGATCTAGGAGAAC
CTCTTCACTGGAGCCCCCTTCCATTACCAACTGTTGATGAGCTAATAATTGCTATTCACACTCTCCCATGGATCCAATTA
ATCAGCAGCACGATGTTGCTAACAGGGTTAGCAGCAGGAATCGCTATCGGAACACCGCCACTAGCAATGATGCTTTGGCC
AAGCCGACTGGGAATAGCAATACAAAGAATCATCTGGGGTCTGCTAAGACTGATGCCAACCCCTCTTACTGCTCTATTTT
TGCTGCTCTTCAGCAAACCGAGTGTGGCTGTTGCTGCTCTTGCACTTGGAGCCCACAACATTGGAGTGATGGGTCGCCTC
TTAAAAGAAGGGCTTGACCAACAAAGCGACAACAACTTGAAAGCCTTAAGCGCGACTGGCGCTGGAGAACGATCGGCTTG
GCTCTATGGATGCTTTAGCGGCCAAAGCCGTAGCTATTTAGCCTATGCCGCTTACAGAACGGACGTGATTTTGCGTGAAA
CCGTGGTAGTTGGCATGGTTGGAGGCACTGGACTGGGTTGGCAGTTAATCGAATCTCTTGGTTCGTTCAACTGGGCACAG
GTCAGTGTCCTAATCGCGGTGTTCACAGCTCTGACCTTGATCGGAGAATCGATCACTGATCGCTTGCGATATCACTGGAC
TGGTTCAACTAAAAAGCTACCTATGGTTGTAGACCTACAAAGCTGA

Upstream 100 bases:

>100_bases
ACTTTGACAGGGTAATCGCACTTAAGTCTGGGAGAGTTGTCTTTGATCAACCTGCTGAATGTCTTGATGAAGAAGCTTTA
GCCTTGATCTACGGATCTGC

Downstream 100 bases:

>100_bases
TCAGGTTCCACTTCTCGATGTGATGGCCAATACATACAAGAGACTCGTTGTCATAGGTGACAGTGGTGTTTATGGATGGG
GAGATCGAGAGGGTGGCGGA

Product: phosphonate ABC transporter

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSAFRGIQITLATALLSWSISSIVG
ICLGIASARVVWETFLGTNWPAIVIRRLLAIPRAIHELLWGLLLLQMVGLSPWVAIVAIVIPYSSLVARVVSDQLDTQDR
RALRGLKQAGAGSSSALITALGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTDLQLALQSLQFHDVWTALWLLAA
VMVSLEQILSLTRRYFKLSKNGIWQVASILIVLLILCALSIPWLNAVDLDLGEPLHWSPLPLPTVDELIIAIHTLPWIQL
ISSTMLLTGLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWGLLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRL
LKEGLDQQSDNNLKALSATGAGERSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS

Sequences:

>Translated_521_residues
MSRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSAFRGIQITLATALLSWSISSIVG
ICLGIASARVVWETFLGTNWPAIVIRRLLAIPRAIHELLWGLLLLQMVGLSPWVAIVAIVIPYSSLVARVVSDQLDTQDR
RALRGLKQAGAGSSSALITALGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTDLQLALQSLQFHDVWTALWLLAA
VMVSLEQILSLTRRYFKLSKNGIWQVASILIVLLILCALSIPWLNAVDLDLGEPLHWSPLPLPTVDELIIAIHTLPWIQL
ISSTMLLTGLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWGLLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRL
LKEGLDQQSDNNLKALSATGAGERSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS
>Mature_520_residues
SRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSAFRGIQITLATALLSWSISSIVGI
CLGIASARVVWETFLGTNWPAIVIRRLLAIPRAIHELLWGLLLLQMVGLSPWVAIVAIVIPYSSLVARVVSDQLDTQDRR
ALRGLKQAGAGSSSALITALGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTDLQLALQSLQFHDVWTALWLLAAV
MVSLEQILSLTRRYFKLSKNGIWQVASILIVLLILCALSIPWLNAVDLDLGEPLHWSPLPLPTVDELIIAIHTLPWIQLI
SSTMLLTGLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWGLLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRLL
KEGLDQQSDNNLKALSATGAGERSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQV
SVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56090; Mature: 55959

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50928 ABC_TM1 ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSAFR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
GIQITLATALLSWSISSIVGICLGIASARVVWETFLGTNWPAIVIRRLLAIPRAIHELLW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLLLLQMVGLSPWVAIVAIVIPYSSLVARVVSDQLDTQDRRALRGLKQAGAGSSSALITA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
LGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTDLQLALQSLQFHDVWTALWLLAA
HHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VMVSLEQILSLTRRYFKLSKNGIWQVASILIVLLILCALSIPWLNAVDLDLGEPLHWSPL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCC
PLPTVDELIIAIHTLPWIQLISSTMLLTGLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHH
LLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRLLKEGLDQQSDNNLKALSATG
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCC
AGERSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
CCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHH
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECC
>Mature Secondary Structure 
SRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSAFR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
GIQITLATALLSWSISSIVGICLGIASARVVWETFLGTNWPAIVIRRLLAIPRAIHELLW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLLLLQMVGLSPWVAIVAIVIPYSSLVARVVSDQLDTQDRRALRGLKQAGAGSSSALITA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
LGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTDLQLALQSLQFHDVWTALWLLAA
HHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VMVSLEQILSLTRRYFKLSKNGIWQVASILIVLLILCALSIPWLNAVDLDLGEPLHWSPL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCC
PLPTVDELIIAIHTLPWIQLISSTMLLTGLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHH
LLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRLLKEGLDQQSDNNLKALSATG
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCC
AGERSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
CCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHH
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]