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The map label for this gene is dapF

Identifier: 33862988

GI number: 33862988

Start: 775463

End: 776374

Strand: Direct

Name: dapF

Synonym: PMT0716

Alternate gene names: 33862988

Gene position: 775463-776374 (Clockwise)

Preceding gene: 33862986

Following gene: 33862989

Centisome position: 32.17

GC content: 47.48

Gene sequence:

>912_bases
ATGCTGGTTATGTTGCAGTTCAGCAAATATCAGGGACTCGGCAACGACTTCCTCCTGATTGATGGGCGTGAGGATCAGCT
GACTCAACAAGTGATCAATCCTGATCCAGCCTGGGTACGAAAAATCTGTGATCGACACTTCGGGATTGGAGCTGATGGGC
TGATTCTTGCTTTACCACCTAGGGCTGATGGTGATCTGAGAATGCAAATTTTCAATGCTGATGGCAGCCTGGCCGAGATG
TGTGGCAATGGCATCCGTTGCTTAACACGTTTCTTAGCTGATATCGAAGGAGATTTGTGTGTTCAGCGGTGGAACATAGA
AACCCTAGCAGGAATAATTTGTCCAGTTTTGCAGGAAGATGGACAAATTTGCGTCGATATGGGAACTCCATTCTTAGATC
CAGAATCGATACCTACCACTCTGACAATCGGTTCTGCTGGATTGCCCCAGGGCGAGTGCCACTTGGGTAGTACATCATTG
CATGTTGCAGCAGTAGGAATGGGTAATCCCCACTTGATCGTGCCTGTTGAAGATCTTGAAAATATCCCCTTTGAAAATTG
GGGACAAAGACTTGAAAAACATCACGCCTTCCCAGCCAAGACAAATGTTCATTTCTTGAAGATACACAGCCCGAATCAAC
TTGAAATTCGCGTTTGGGAGCGTGGGTCGGGACCAACCCTGGCTTGTGGTACAGGAGCCTGTGCATCTCTTGTCGCAACT
TGTTTGCTAGGTCTTTCTGATGACCATGCGGAGGTTCTTTTACCAGGTGGTGTATTACAGATCAGTTGGCCAGGACGTAG
GGGTTCTGTTTTTATGACGGGGCCCGCTGAACCTATCTTTGACGGGGTTTTAACACCTCTTCTCTCACCAAGTCATGCTG
AGGTCTTGCCTCAAGATGATCAGATTATTTGA

Upstream 100 bases:

>100_bases
AGACTGAGAGGGATCTCGTCCCTAACCCAACTCTGAATCAGACGAATCCCTGGAAGGCTGGGATTGAGAGGGGGGGGCTC
CATAGGATTGGGATCGTTAC

Downstream 100 bases:

>100_bases
AGACATCAAATAGTCTACAAACATTTATAGCTCTTGAATTTATAGAGACTCCTATCTTGGATAAGATAACAACCCTGAGG
TTGATTCCCTTAAAATGGTG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLVMLQFSKYQGLGNDFLLIDGREDQLTQQVINPDPAWVRKICDRHFGIGADGLILALPPRADGDLRMQIFNADGSLAEM
CGNGIRCLTRFLADIEGDLCVQRWNIETLAGIICPVLQEDGQICVDMGTPFLDPESIPTTLTIGSAGLPQGECHLGSTSL
HVAAVGMGNPHLIVPVEDLENIPFENWGQRLEKHHAFPAKTNVHFLKIHSPNQLEIRVWERGSGPTLACGTGACASLVAT
CLLGLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPIFDGVLTPLLSPSHAEVLPQDDQII

Sequences:

>Translated_303_residues
MLVMLQFSKYQGLGNDFLLIDGREDQLTQQVINPDPAWVRKICDRHFGIGADGLILALPPRADGDLRMQIFNADGSLAEM
CGNGIRCLTRFLADIEGDLCVQRWNIETLAGIICPVLQEDGQICVDMGTPFLDPESIPTTLTIGSAGLPQGECHLGSTSL
HVAAVGMGNPHLIVPVEDLENIPFENWGQRLEKHHAFPAKTNVHFLKIHSPNQLEIRVWERGSGPTLACGTGACASLVAT
CLLGLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPIFDGVLTPLLSPSHAEVLPQDDQII
>Mature_303_residues
MLVMLQFSKYQGLGNDFLLIDGREDQLTQQVINPDPAWVRKICDRHFGIGADGLILALPPRADGDLRMQIFNADGSLAEM
CGNGIRCLTRFLADIEGDLCVQRWNIETLAGIICPVLQEDGQICVDMGTPFLDPESIPTTLTIGSAGLPQGECHLGSTSL
HVAAVGMGNPHLIVPVEDLENIPFENWGQRLEKHHAFPAKTNVHFLKIHSPNQLEIRVWERGSGPTLACGTGACASLVAT
CLLGLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPIFDGVLTPLLSPSHAEVLPQDDQII

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=35.5871886120996, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PROMM (Q7V7M5)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894548.1
- HSSP:   P44859
- ProteinModelPortal:   Q7V7M5
- SMR:   Q7V7M5
- STRING:   Q7V7M5
- GeneID:   1728549
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0716
- NMPDR:   fig|74547.1.peg.715
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   NACGTAM
- ProtClustDB:   PRK00450
- BioCyc:   PMAR74547:PMT0716-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 32747; Mature: 32747

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 81-81 ACT_SITE 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVMLQFSKYQGLGNDFLLIDGREDQLTQQVINPDPAWVRKICDRHFGIGADGLILALPP
CEEEEEEHHCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECC
RADGDLRMQIFNADGSLAEMCGNGIRCLTRFLADIEGDLCVQRWNIETLAGIICPVLQED
CCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCC
GQICVDMGTPFLDPESIPTTLTIGSAGLPQGECHLGSTSLHVAAVGMGNPHLIVPVEDLE
CCEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEECCCEEEEEEEECCCCEEEEEHHHHC
NIPFENWGQRLEKHHAFPAKTNVHFLKIHSPNQLEIRVWERGSGPTLACGTGACASLVAT
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHHH
CLLGLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPIFDGVLTPLLSPSHAEVLPQDD
HHHCCCCCCCEEEECCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
QII
CCC
>Mature Secondary Structure
MLVMLQFSKYQGLGNDFLLIDGREDQLTQQVINPDPAWVRKICDRHFGIGADGLILALPP
CEEEEEEHHCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECC
RADGDLRMQIFNADGSLAEMCGNGIRCLTRFLADIEGDLCVQRWNIETLAGIICPVLQED
CCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCC
GQICVDMGTPFLDPESIPTTLTIGSAGLPQGECHLGSTSLHVAAVGMGNPHLIVPVEDLE
CCEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEECCCEEEEEEEECCCCEEEEEHHHHC
NIPFENWGQRLEKHHAFPAKTNVHFLKIHSPNQLEIRVWERGSGPTLACGTGACASLVAT
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHHH
CLLGLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPIFDGVLTPLLSPSHAEVLPQDD
HHHCCCCCCCEEEECCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
QII
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642