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The map label for this gene is nifS [H]

Identifier: 33862989

GI number: 33862989

Start: 776566

End: 777765

Strand: Direct

Name: nifS [H]

Synonym: PMT0717

Alternate gene names: 33862989

Gene position: 776566-777765 (Clockwise)

Preceding gene: 33862988

Following gene: 33862990

Centisome position: 32.21

GC content: 50.67

Gene sequence:

>1200_bases
ATGGACAGTTCTAGTTCGTGCCCATCTGAAATATATCTGGATGCTTGTGCAACGACTCCGCCTCGACCAGAGGTGATTCA
ACGCATCTTGGAAGTTCAGGAGAGTGCCTGGGGTAACCCCTCGAGTCTTCATGGCCCAGGTCTGATCGCTGCTGAATTAC
TTGAACGCAGTCGTCAGAAGATTGCCTCATGCCTAATGGCTAGCTCAACCGAGCTGGTCTTGACCTCGGGAGCTACAGAA
TCTATTCATTTAGGCCTTTTGGGCATGGCTCGTTCCATGCAGTCGGGCCGTCTAGTGATTTCAGCGGTAGAACATCCTGC
TGTTCTTGCTGCAGCTCAGGCCCTCAAGCGTGAGGGATGGGAGGTGTGTTACTGGCCTGTTGATCCACTAGGTCGCGTCA
CACTTGAGGATCTAGATCTGATGCTTGCTGCGCCAACACGATTGGTCTCAATCATCTGGGGGCAAAGTGAGGTCGGTACC
ATTCAACCGATTGAGACGATTGGCTTAGCTTGTAGGGAAAGGGGCATTGTTTTTCACACTGATGCCACTCAAGTTCTTAG
TCAGGGACTACTGCATTGGGCTGATCTGCCAATAGACTTACTTAGCGCATCAGCACACAAGTTTCAGGGTCCTAAGGGAA
TTGGTTTGCTTCTATTAAGACCTGAATTGATAGATGTTTTACAACCTTTGCAGGGTGGTGGTGGCCAAGAACAGGGGCTT
CGGGCTGGTACTGAATCTGTCGCCTTAGCAGCAGGGATGGCGATGGCCCTCGAACAATTGAAACTATCCAGGTCAGAGTG
CGCTGATGTTGAGCTGGCCGATCAGGTGAAAGTTCAACGAATGCGTGACTCGTTAAGAATCAACCTGCAGAATTTTCCTG
GACTTCACTTCACCGGCGATCCAATCAATCGTTTGCCTCACCATATATCCATGCTTGTGGGATCAGGAGATGACCAGCCC
ATCTCTGGTAGAGCTGTTGTGCGAGAACTTTCACGACTTGGCGTGGCAACAAGTAGCGGTAGTGCCTGTATGGCAGGGCA
ACCTAAAAACAGTGCTGTCCTGCAGGCCATGCAGATCAAACCAGAGTGGCTTCAGTCAGGATTACGTTTCTCTCTGGGTT
CATGGTTAGACGAAACACAGTTGGATCAGATCCCTGATCTTCTGCAGCAGGCCATGATTGCAGCAGCATCGCCAGAATGA

Upstream 100 bases:

>100_bases
AAAATGGTGAATGATTGTTTAACTAGTATCTTGTTAGAGAGCTTGATCGCTCCTGCTTTTGATGCATTTGGGCATCACAC
TCGCATCGGTCTAGGAAAGG

Downstream 100 bases:

>100_bases
GCCTTACTTCTAATCCTTAAATCATCCACCCCAGCCTGTGATAAAAACTCTTCCCGCTGACCTCCTCGAAGCCGAGAACA
TGGTTTTGGAGGCTCTACAA

Product: class-V aminotransferase family cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MDSSSSCPSEIYLDACATTPPRPEVIQRILEVQESAWGNPSSLHGPGLIAAELLERSRQKIASCLMASSTELVLTSGATE
SIHLGLLGMARSMQSGRLVISAVEHPAVLAAAQALKREGWEVCYWPVDPLGRVTLEDLDLMLAAPTRLVSIIWGQSEVGT
IQPIETIGLACRERGIVFHTDATQVLSQGLLHWADLPIDLLSASAHKFQGPKGIGLLLLRPELIDVLQPLQGGGGQEQGL
RAGTESVALAAGMAMALEQLKLSRSECADVELADQVKVQRMRDSLRINLQNFPGLHFTGDPINRLPHHISMLVGSGDDQP
ISGRAVVRELSRLGVATSSGSACMAGQPKNSAVLQAMQIKPEWLQSGLRFSLGSWLDETQLDQIPDLLQQAMIAAASPE

Sequences:

>Translated_399_residues
MDSSSSCPSEIYLDACATTPPRPEVIQRILEVQESAWGNPSSLHGPGLIAAELLERSRQKIASCLMASSTELVLTSGATE
SIHLGLLGMARSMQSGRLVISAVEHPAVLAAAQALKREGWEVCYWPVDPLGRVTLEDLDLMLAAPTRLVSIIWGQSEVGT
IQPIETIGLACRERGIVFHTDATQVLSQGLLHWADLPIDLLSASAHKFQGPKGIGLLLLRPELIDVLQPLQGGGGQEQGL
RAGTESVALAAGMAMALEQLKLSRSECADVELADQVKVQRMRDSLRINLQNFPGLHFTGDPINRLPHHISMLVGSGDDQP
ISGRAVVRELSRLGVATSSGSACMAGQPKNSAVLQAMQIKPEWLQSGLRFSLGSWLDETQLDQIPDLLQQAMIAAASPE
>Mature_399_residues
MDSSSSCPSEIYLDACATTPPRPEVIQRILEVQESAWGNPSSLHGPGLIAAELLERSRQKIASCLMASSTELVLTSGATE
SIHLGLLGMARSMQSGRLVISAVEHPAVLAAAQALKREGWEVCYWPVDPLGRVTLEDLDLMLAAPTRLVSIIWGQSEVGT
IQPIETIGLACRERGIVFHTDATQVLSQGLLHWADLPIDLLSASAHKFQGPKGIGLLLLRPELIDVLQPLQGGGGQEQGL
RAGTESVALAAGMAMALEQLKLSRSECADVELADQVKVQRMRDSLRINLQNFPGLHFTGDPINRLPHHISMLVGSGDDQP
ISGRAVVRELSRLGVATSSGSACMAGQPKNSAVLQAMQIKPEWLQSGLRFSLGSWLDETQLDQIPDLLQQAMIAAASPE

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=377, Percent_Identity=34.2175066312997, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI156713448, Length=430, Percent_Identity=27.906976744186, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=33.5917312661499, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787970, Length=353, Percent_Identity=26.0623229461756, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789175, Length=282, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=34.7150259067358, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=26.7477203647416, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319831, Length=377, Percent_Identity=35.0132625994695, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI20129463, Length=379, Percent_Identity=34.5646437994723, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42768; Mature: 42768

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSSSCPSEIYLDACATTPPRPEVIQRILEVQESAWGNPSSLHGPGLIAAELLERSRQK
CCCCCCCCHHHEEHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
IASCLMASSTELVLTSGATESIHLGLLGMARSMQSGRLVISAVEHPAVLAAAQALKREGW
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCC
EVCYWPVDPLGRVTLEDLDLMLAAPTRLVSIIWGQSEVGTIQPIETIGLACRERGIVFHT
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEC
DATQVLSQGLLHWADLPIDLLSASAHKFQGPKGIGLLLLRPELIDVLQPLQGGGGQEQGL
CHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCEEEEECHHHHHHHHHHCCCCCCCHHH
RAGTESVALAAGMAMALEQLKLSRSECADVELADQVKVQRMRDSLRINLQNFPGLHFTGD
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
PINRLPHHISMLVGSGDDQPISGRAVVRELSRLGVATSSGSACMAGQPKNSAVLQAMQIK
HHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHCC
PEWLQSGLRFSLGSWLDETQLDQIPDLLQQAMIAAASPE
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSSSSCPSEIYLDACATTPPRPEVIQRILEVQESAWGNPSSLHGPGLIAAELLERSRQK
CCCCCCCCHHHEEHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
IASCLMASSTELVLTSGATESIHLGLLGMARSMQSGRLVISAVEHPAVLAAAQALKREGW
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCC
EVCYWPVDPLGRVTLEDLDLMLAAPTRLVSIIWGQSEVGTIQPIETIGLACRERGIVFHT
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEC
DATQVLSQGLLHWADLPIDLLSASAHKFQGPKGIGLLLLRPELIDVLQPLQGGGGQEQGL
CHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCEEEEECHHHHHHHHHHCCCCCCCHHH
RAGTESVALAAGMAMALEQLKLSRSECADVELADQVKVQRMRDSLRINLQNFPGLHFTGD
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
PINRLPHHISMLVGSGDDQPISGRAVVRELSRLGVATSSGSACMAGQPKNSAVLQAMQIK
HHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHCC
PEWLQSGLRFSLGSWLDETQLDQIPDLLQQAMIAAASPE
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA