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The map label for this gene is leuB

Identifier: 33862804

GI number: 33862804

Start: 579084

End: 580157

Strand: Direct

Name: leuB

Synonym: PMT0531

Alternate gene names: 33862804

Gene position: 579084-580157 (Clockwise)

Preceding gene: 33862803

Following gene: 33862805

Centisome position: 24.02

GC content: 55.21

Gene sequence:

>1074_bases
ATGCGCCAGCACCGCATCGTTCTGCTGCCTGGTGATGGTATCGGTCCTGAAATCACTGCTGTAGCAAAGCTTTTACTTGA
TGCATTAGGCCACCAACACGGTTTCAAGCTGAATTTCGAGCAACACCCCATCGGTGGGGTTGCCATAGACGCAAGCGGAT
CGCCCTTGCCCGCCAGCACCCTTGAGGCATGTCAAGCCAGTGATGCGGTGCTCCTTGCAGCAATTGGTAGCCCTCGCTTT
GACGCCCTGCCCAGGGAGCAACGGCCGGAAACAGGCTTGCTATCCCTTCGGGCAGGACTGAAACTATTTGCGAACCTCAG
GCCGGTGACGATCCTGCCGGCCCTGATCGATGCCAGCAGCCTGAAAGCAAATGTGATCAAAGGCGTTGATCTAATGGTGG
TGCGTGAGCTCACAGGTGGGATTTATTTCGGCCAACCGAAAGGGCGGATTGAGGCCGATGGGGATGAGCGCGCTTTCAAC
ACAATGACCTACTCCAGAACTGAGGTGGATCGAATCGCCAAGGTCGCTTTCGAGCTCGCCCGCGATCGCAGCGGAAAGCT
TTGTTCAGTAGATAAGGCAAACGTGCTTGATGTCAGTCAACTGTGGCGTGATCGTGTCGATGCTTTGGCTGCCAACTATG
GAGATGTAGAGCTCAGCCACATGTATGTGGACAATGCCGCGATGCAATTGGTGCGCAACCCCAGGCAATTTGACGTGCTG
CTTACCGGCAACCTATTCGGAGACATCCTTAGTGACGAAGCAGCGATGCTTACCGGTTCGATTGGCATGCTGCCCTCAGC
TTCGCTCGGCAGCGAAGGACCAGGACTGTTTGAACCGGTGCATGGCTCCGCACCCGATATTGCAGGACAAGACCTGGCCA
ATCCAATGGCAATGGTGCTTTGCGCAGCAATGATGCTGAGAATTGGCTTAAAGGAAAACGATGCCGCCAGGGCTCTTGAA
GGATCAGTAGAGCGTGTTCTCGCGGCAGGTTTCCGTACAGGTGACCTCATGAGTGAGGGTTGTACCCAACTGGGCTGCGC
AGAGATGGGTGAGCAATTGCTGCAAGCGCTTTAA

Upstream 100 bases:

>100_bases
TTCAGCAAATTACCGGAGATGGCCAAAATGTTGAGGAAGCTGACAAGAGATACACCTCAGTAACCTCACAGGAGGCACAA
GCCATTTTGGAGTTCTCCAC

Downstream 100 bases:

>100_bases
AGGATCGAGAACTCTGATAAGCAAATGCTGATGTTGCCAAATTGAGCTGTTTGCTCCATGACCTGCCAAAATCTCTGGGC
CTGTCCTGTCTGCGTCGATG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRQHRIVLLPGDGIGPEITAVAKLLLDALGHQHGFKLNFEQHPIGGVAIDASGSPLPASTLEACQASDAVLLAAIGSPRF
DALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASSLKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFN
TMTYSRTEVDRIAKVAFELARDRSGKLCSVDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVLCAAMMLRIGLKENDAARALE
GSVERVLAAGFRTGDLMSEGCTQLGCAEMGEQLLQAL

Sequences:

>Translated_357_residues
MRQHRIVLLPGDGIGPEITAVAKLLLDALGHQHGFKLNFEQHPIGGVAIDASGSPLPASTLEACQASDAVLLAAIGSPRF
DALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASSLKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFN
TMTYSRTEVDRIAKVAFELARDRSGKLCSVDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVLCAAMMLRIGLKENDAARALE
GSVERVLAAGFRTGDLMSEGCTQLGCAEMGEQLLQAL
>Mature_357_residues
MRQHRIVLLPGDGIGPEITAVAKLLLDALGHQHGFKLNFEQHPIGGVAIDASGSPLPASTLEACQASDAVLLAAIGSPRF
DALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASSLKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFN
TMTYSRTEVDRIAKVAFELARDRSGKLCSVDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVLCAAMMLRIGLKENDAARALE
GSVERVLAAGFRTGDLMSEGCTQLGCAEMGEQLLQAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=331, Percent_Identity=32.9305135951662, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI28178821, Length=308, Percent_Identity=30.1948051948052, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=30.6451612903226, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4758582, Length=307, Percent_Identity=28.0130293159609, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178838, Length=307, Percent_Identity=28.0130293159609, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI28178819, Length=145, Percent_Identity=37.2413793103448, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=51.9886363636364, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788101, Length=352, Percent_Identity=36.6477272727273, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1787381, Length=404, Percent_Identity=25, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=351, Percent_Identity=33.9031339031339, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=319, Percent_Identity=30.4075235109718, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=31.6568047337278, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=307, Percent_Identity=29.6416938110749, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=46.1971830985916, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=34.7457627118644, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=349, Percent_Identity=30.3724928366762, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=31.547619047619, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=34.0236686390533, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643270, Length=338, Percent_Identity=34.0236686390533, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24661184, Length=369, Percent_Identity=31.9783197831978, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=28.5714285714286, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=28.5714285714286, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=26.6081871345029, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=26.6081871345029, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=26.6081871345029, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=26.6081871345029, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=26.7062314540059, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_PROMM (Q7V842)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894364.1
- ProteinModelPortal:   Q7V842
- SMR:   Q7V842
- STRING:   Q7V842
- GeneID:   1729525
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0531
- NMPDR:   fig|74547.1.peg.531
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   DERAFNT
- ProtClustDB:   PRK00772
- BioCyc:   PMAR74547:PMT0531-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 37956; Mature: 37956

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 97-97 BINDING 107-107 BINDING 135-135 BINDING 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHRIVLLPGDGIGPEITAVAKLLLDALGHQHGFKLNFEQHPIGGVAIDASGSPLPAST
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCCCCCHHH
LEACQASDAVLLAAIGSPRFDALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASS
HHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFNTMTYSRTEVDRIAKVAFELA
HHHHHHCCCHHHHHHHHHCCEEECCCCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHHHH
RDRSGKLCSVDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
HCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCCCEEEE
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVL
EECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
CAAMMLRIGLKENDAARALEGSVERVLAAGFRTGDLMSEGCTQLGCAEMGEQLLQAL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRQHRIVLLPGDGIGPEITAVAKLLLDALGHQHGFKLNFEQHPIGGVAIDASGSPLPAST
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCCCCCHHH
LEACQASDAVLLAAIGSPRFDALPREQRPETGLLSLRAGLKLFANLRPVTILPALIDASS
HHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKANVIKGVDLMVVRELTGGIYFGQPKGRIEADGDERAFNTMTYSRTEVDRIAKVAFELA
HHHHHHCCCHHHHHHHHHCCEEECCCCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHHHH
RDRSGKLCSVDKANVLDVSQLWRDRVDALAANYGDVELSHMYVDNAAMQLVRNPRQFDVL
HCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCCCEEEE
LTGNLFGDILSDEAAMLTGSIGMLPSASLGSEGPGLFEPVHGSAPDIAGQDLANPMAMVL
EECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
CAAMMLRIGLKENDAARALEGSVERVLAAGFRTGDLMSEGCTQLGCAEMGEQLLQAL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642