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The map label for this gene is sigB [H]

Identifier: 33862729

GI number: 33862729

Start: 512667

End: 513593

Strand: Direct

Name: sigB [H]

Synonym: PMT0456

Alternate gene names: 33862729

Gene position: 512667-513593 (Clockwise)

Preceding gene: 33862726

Following gene: 33862730

Centisome position: 21.26

GC content: 50.27

Gene sequence:

>927_bases
ATGGTGAGTTCTTTAAGTGCCTATCTTGGGGAGATCGGGCGACATCAACTTTTGACACCTGAACAGGAACTGACAATGGG
TCGGAAGGTTCAGGCCATGGTTGCGCTTACTAACCGATGCCATCTTGCTGGTGGAGCAGGTCCCGAATGCGAATACTCAG
ATGATCAGCGCCTCACCATTCGTCGTGGTGAAAAAGCCAAGAATCAGATGATCACTGCCAATTTAAGACTTGTAGTGAAT
CTTGCCAAGCGCTACCAGGGCAAGGGGCTCGATCTTCTCGATCTGATTCAAGAAGGCACGCTTGGACTTACCAGAGCAGT
GGAGAAATATGACCCAACCCGTGGCCATAGATTTTCGACTTATGCCTATTGGTGGATTCGCCAGGGGCTTAACCGGGCAC
TATCGACTCAGAGCAGGACGATACGAATTCCCGTCAATGTGAATGAGAAGCTAACCAAATTAAGGGCAGCAAAGTCAAGA
CTGATGCAGAACAACGGCTTGCCTCCCACTGCTGAGCAATTGGCAAAAACCATGCGACTGCCGATGGCTGAAGTGGAAGA
TCTGCTCGCTTGTGAATTACGCAGTGTGACAGTGAGCCTTCAAGGAGTTGTGAAGTCAAAATCCGATCCTTCAGAACTTG
CGGATGTCCTTCCCAGCGAAGAGATCCCTCCTATGGAGCGTGCCGAAATGTCGGAAAGAACTGCATCGGTATGGACTTTG
CTCAATCGTGCGAACCTCACTCCTAAAGAACGGATGGTGGTCACACTTCGTTTTGGCCTCGACGGATCTCATGAATGGCG
CACTCTCGCGGAAGTTGCACGACATATGAGTTGCAGTCGCGAATACTGCAGGCAGGTATTACAAAGGGCACTGCGAAAAC
TGCGCAAAGCAGGAATGCAGAGTGGTTTGGTAGAAAGCACCCTCTAA

Upstream 100 bases:

>100_bases
AACTGTTTAATAACGCATAAAAAGTTAATTCCGTACTCTTCCTTACGCAGTCAGGATGGAAACACTTTGGCTCTAGGTTC
AATTAAGAGTTGAGTTCCAG

Downstream 100 bases:

>100_bases
GCGATAAACCTCACTAAAAAGTGGGAAATCCGCCCCAAGAAGGGGCGGAGCTCATCTTATGAATCCCCATAAATCAGGTA
AAAATGGAATGACATCGGCG

Product: RNA polymerase sigma-70 factor family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTNRCHLAGGAGPECEYSDDQRLTIRRGEKAKNQMITANLRLVVN
LAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSR
LMQNNGLPPTAEQLAKTMRLPMAEVEDLLACELRSVTVSLQGVVKSKSDPSELADVLPSEEIPPMERAEMSERTASVWTL
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVLQRALRKLRKAGMQSGLVESTL

Sequences:

>Translated_308_residues
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTNRCHLAGGAGPECEYSDDQRLTIRRGEKAKNQMITANLRLVVN
LAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSR
LMQNNGLPPTAEQLAKTMRLPMAEVEDLLACELRSVTVSLQGVVKSKSDPSELADVLPSEEIPPMERAEMSERTASVWTL
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVLQRALRKLRKAGMQSGLVESTL
>Mature_308_residues
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTNRCHLAGGAGPECEYSDDQRLTIRRGEKAKNQMITANLRLVVN
LAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSR
LMQNNGLPPTAEQLAKTMRLPMAEVEDLLACELRSVTVSLQGVVKSKSDPSELADVLPSEEIPPMERAEMSERTASVWTL
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVLQRALRKLRKAGMQSGLVESTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=39.1489361702128, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789098, Length=300, Percent_Identity=37.6666666666667, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.5123966942149, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34671; Mature: 34671

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTNRCHLAGGAGPECEYSDDQRLTI
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEE
RRGEKAKNQMITANLRLVVNLAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFST
ECCCHHHHHEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCHH
YAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSRLMQNNGLPPTAEQLAKTMRL
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PMAEVEDLLACELRSVTVSLQGVVKSKSDPSELADVLPSEEIPPMERAEMSERTASVWTL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVLQRALRKLRKAGMQ
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVESTL
CCCHHHCC
>Mature Secondary Structure
MVSSLSAYLGEIGRHQLLTPEQELTMGRKVQAMVALTNRCHLAGGAGPECEYSDDQRLTI
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEE
RRGEKAKNQMITANLRLVVNLAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPTRGHRFST
ECCCHHHHHEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCHH
YAYWWIRQGLNRALSTQSRTIRIPVNVNEKLTKLRAAKSRLMQNNGLPPTAEQLAKTMRL
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PMAEVEDLLACELRSVTVSLQGVVKSKSDPSELADVLPSEEIPPMERAEMSERTASVWTL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LNRANLTPKERMVVTLRFGLDGSHEWRTLAEVARHMSCSREYCRQVLQRALRKLRKAGMQ
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLVESTL
CCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA