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The map label for this gene is hisI

Identifier: 33862726

GI number: 33862726

Start: 510604

End: 511272

Strand: Direct

Name: hisI

Synonym: PMT0453

Alternate gene names: 33862726

Gene position: 510604-511272 (Clockwise)

Preceding gene: 33862725

Following gene: 33862729

Centisome position: 21.18

GC content: 49.78

Gene sequence:

>669_bases
ATGCAACCCCTCAGCCCCGCCTTCATCGACCAATTGTGTTTTGACGATATTGGTTTGATTCCAGCGATCTCCCAAGACTG
GCTTGATGGAGCCGTTCTGATGATGGCCTGGATGAATCGCACTGCCTTGGAGCAGACATTAAAGAGTGGCCAAGTTCATT
ACTGGAGCCGGTCGCGTCAGGAGCTTTGGCATAAAGGTGCTACGAGCGGTCATACGCAGATCCTTAAGGGAATTCGTTAT
GACTGCGATGCTGATGTGCTTTTACTCAGTATCGAACAAACCGGTTTGGTCAGTTGTCATACCGGAGCCCGAAGCTGTTT
CTTCGCAGAGGTAAACCAGCATTCGCAGGGAGACTCACTCACTCTTCCACCTCCCATGGATGCATGCAGTGAACTTTTTC
GGGTGATTGATCAACGCCACACCACACCCGAAGCCAACAGTTATACAAACAAGCTTTTAGAAGGTGGAGACAATCGCATC
CTCAAAAAAATTGGTGAGGAGAGTGCGGAATTTGTGATGGCTTGCAAAGACGACGATGAGAAGGCAATTGCTAATGAAGC
GGCTGATCTTCTCTTTCATCTGCAAGTCGCTCTTGCCCACCATGGTGTGAACTGGAGAGATGTGCTTGAGGTGCTCGCTA
ATAGACGTGGAGCACCGCGTCGAAACTGA

Upstream 100 bases:

>100_bases
GCCTTAGGAGGGGCGGATCATGCGAGAGCGAAGCACAGTTGATGACTAGTTTGGTGCTGAGGGATGATTGCACGGCCGCT
CCTGCGCTCCTTGAGGACGA

Downstream 100 bases:

>100_bases
ATGCTGAACTGTGGCTGATTGAGTGATTCTTTTTAGTGAAATTCCTATCAACGTGGTTGCCCAAAGTTGGTGGATTGTGA
ACGGATCCACTCAAGTGTGA

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MQPLSPAFIDQLCFDDIGLIPAISQDWLDGAVLMMAWMNRTALEQTLKSGQVHYWSRSRQELWHKGATSGHTQILKGIRY
DCDADVLLLSIEQTGLVSCHTGARSCFFAEVNQHSQGDSLTLPPPMDACSELFRVIDQRHTTPEANSYTNKLLEGGDNRI
LKKIGEESAEFVMACKDDDEKAIANEAADLLFHLQVALAHHGVNWRDVLEVLANRRGAPRRN

Sequences:

>Translated_222_residues
MQPLSPAFIDQLCFDDIGLIPAISQDWLDGAVLMMAWMNRTALEQTLKSGQVHYWSRSRQELWHKGATSGHTQILKGIRY
DCDADVLLLSIEQTGLVSCHTGARSCFFAEVNQHSQGDSLTLPPPMDACSELFRVIDQRHTTPEANSYTNKLLEGGDNRI
LKKIGEESAEFVMACKDDDEKAIANEAADLLFHLQVALAHHGVNWRDVLEVLANRRGAPRRN
>Mature_222_residues
MQPLSPAFIDQLCFDDIGLIPAISQDWLDGAVLMMAWMNRTALEQTLKSGQVHYWSRSRQELWHKGATSGHTQILKGIRY
DCDADVLLLSIEQTGLVSCHTGARSCFFAEVNQHSQGDSLTLPPPMDACSELFRVIDQRHTTPEANSYTNKLLEGGDNRI
LKKIGEESAEFVMACKDDDEKAIANEAADLLFHLQVALAHHGVNWRDVLEVLANRRGAPRRN

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=198, Percent_Identity=37.3737373737374, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319819, Length=177, Percent_Identity=31.638418079096, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_PROMM (Q7TV28)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894286.1
- ProteinModelPortal:   Q7TV28
- STRING:   Q7TV28
- GeneID:   1729694
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0453
- NMPDR:   fig|74547.1.peg.453
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BioCyc:   PMAR74547:PMT0453-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 24827; Mature: 24827

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLSPAFIDQLCFDDIGLIPAISQDWLDGAVLMMAWMNRTALEQTLKSGQVHYWSRSRQ
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
ELWHKGATSGHTQILKGIRYDCDADVLLLSIEQTGLVSCHTGARSCFFAEVNQHSQGDSL
HHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCC
TLPPPMDACSELFRVIDQRHTTPEANSYTNKLLEGGDNRILKKIGEESAEFVMACKDDDE
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHEEEECCCCCH
KAIANEAADLLFHLQVALAHHGVNWRDVLEVLANRRGAPRRN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQPLSPAFIDQLCFDDIGLIPAISQDWLDGAVLMMAWMNRTALEQTLKSGQVHYWSRSRQ
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
ELWHKGATSGHTQILKGIRYDCDADVLLLSIEQTGLVSCHTGARSCFFAEVNQHSQGDSL
HHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCC
TLPPPMDACSELFRVIDQRHTTPEANSYTNKLLEGGDNRILKKIGEESAEFVMACKDDDE
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHCCHHHHEEEECCCCCH
KAIANEAADLLFHLQVALAHHGVNWRDVLEVLANRRGAPRRN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642