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The map label for this gene is insF1 [C]

Identifier: 30065506

GI number: 30065506

Start: 4455218

End: 4456036

Strand: Direct

Name: insF1 [C]

Synonym: S4551

Alternate gene names: 30065506

Gene position: 4455218-4456036 (Clockwise)

Preceding gene: 30065503

Following gene: 30065508

Centisome position: 96.87

GC content: 51.53

Gene sequence:

>819_bases
ATGTGTCAGGTATTTGGTGTATCCAGGAGCGGTTATTACGACCGGGTACAGCATGCCCCCTCAGACAGAAAACAAAGTGA
TGAGCGGCTAAAACTGGAGATTAAGGTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGCGGCTCCAGACGGAGC
TGGCAGAGAATGGCATCATCGTTGGTCGTGACCGACTGGCACGTCTTCGTAAGGAGCTAAGGCTACGCTGTAAGCAGAAA
CGCAAGTTCAGAGCGACTACGAACCCGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTAC
AGCACCAAATCAGGTCTGGGTGGCGGACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACCTCGCTGGCATCAAAG
ATGTTTATACGTGCGAAATTGTCGGCTACGCCATGGGAGAGCGCATGACAAAAGAGCTGACAGGTAAAGCCCTGTTTATG
GCGCTCAGGAGCCAGCGCCCACCTGCCGGGCTAATCCACCACTCTGATCGAGGTTCACAGTACTGCGCATACGATTACCG
GGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTAAAGGTAACTGTTACGACAACGCTCCGATGGAAAGCT
TCTGGGGAACGCTGAAAAATGAGAGTCTGAGCCACTATCGTTTTAATAACCGGGATGAAGCCATCTCAGTAATACGGGAA
TACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCTGGGGAATATCTCCCCGGCAGCCTTCAGGGAAAAATA
TCATCAGATGGCTGCTTAA

Upstream 100 bases:

>100_bases
CAGTAGCGGAGCAGGAAGGACATCTGATGGAAATGGAAGCCACGCAAGCACCTTAAAATCACCATCATACACTAAATCAG
TAAGTTGGCAGCATTACCCG

Downstream 100 bases:

>100_bases
AAAAAGAACAAATGGTAGTGTCCGCTATTGCCAGTACACCTCACAGGTCAGATGCTTTACCACCGGCAGAGTTCATCAGA
GTGATGAGGATCATCAGGGC

Product: IS600 orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MCQVFGVSRSGYYDRVQHAPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Sequences:

>Translated_272_residues
MCQVFGVSRSGYYDRVQHAPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
>Mature_272_residues
MCQVFGVSRSGYYDRVQHAPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQK
RKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIRE
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=244, Percent_Identity=31.5573770491803, Blast_Score=127, Evalue=8e-30,
Organism=Escherichia coli, GI1788405, Length=268, Percent_Identity=40.6716417910448, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1787263, Length=268, Percent_Identity=40.6716417910448, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1786753, Length=268, Percent_Identity=40.6716417910448, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1786570, Length=268, Percent_Identity=40.6716417910448, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1786490, Length=268, Percent_Identity=40.6716417910448, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1789981, Length=264, Percent_Identity=32.5757575757576, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31557; Mature: 31557

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQVFGVSRSGYYDRVQHAPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
CCEEEECCCCCCHHHHHCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCEE
VGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
EEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQ
EECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
YCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIRE
EEEHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MCQVFGVSRSGYYDRVQHAPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGII
CCEEEECCCCCCHHHHHCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCEE
VGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
EEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEE
ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQ
EECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
YCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIRE
EEEHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
YIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]