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The map label for this gene is insA [H]

Identifier: 30065508

GI number: 30065508

Start: 4457304

End: 4457579

Strand: Direct

Name: insA [H]

Synonym: S4553

Alternate gene names: 30065508

Gene position: 4457304-4457579 (Clockwise)

Preceding gene: 30065506

Following gene: 30065509

Centisome position: 96.91

GC content: 52.54

Gene sequence:

>276_bases
GTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACA
TCAGCGCTATCTCTGCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACC
AGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTT
TTACGTCACTTAAAAAACTCAGGCCGCAGTCGGTAA

Upstream 100 bases:

>100_bases
TGGCGATAAGATTACTCACGAAAAAAGGATTAATCCTAAAGATTAGGTAATGCTGCCAACTTACTGATTTAGTGTATGAT
GGTGATTTTAAGGTGCTTGC

Downstream 100 bases:

>100_bases
CCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACATTGGGGCTACGTCGGTGCTAAATCA
CGTCAGCGCTGGCTGTTTTA

Product: IS1 orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTV
LRHLKNSGRSR

Sequences:

>Translated_91_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTV
LRHLKNSGRSR
>Mature_90_residues
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVL
RHLKNSGRSR

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1790747, Length=91, Percent_Identity=98.9010989010989, Blast_Score=186, Evalue=3e-49,
Organism=Escherichia coli, GI1789852, Length=91, Percent_Identity=93.4065934065934, Blast_Score=182, Evalue=5e-48,
Organism=Escherichia coli, GI1788204, Length=91, Percent_Identity=93.4065934065934, Blast_Score=182, Evalue=5e-48,
Organism=Escherichia coli, GI1786204, Length=91, Percent_Identity=93.4065934065934, Blast_Score=182, Evalue=5e-48,
Organism=Escherichia coli, GI87081814, Length=91, Percent_Identity=92.3076923076923, Blast_Score=181, Evalue=1e-47,
Organism=Escherichia coli, GI1786471, Length=91, Percent_Identity=92.3076923076923, Blast_Score=181, Evalue=1e-47,
Organism=Escherichia coli, GI1786460, Length=91, Percent_Identity=92.3076923076923, Blast_Score=181, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 9965; Mature: 9833

Theoretical pI: Translated: 11.24; Mature: 11.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGKHQKIIDMA
CCCEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
MNGVGCRASARIMGVGLNTVLRHLKNSGRSR
HCCCCCCCCCEEEEECHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGKHQKIIDMA
CCEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
MNGVGCRASARIMGVGLNTVLRHLKNSGRSR
HCCCCCCCCCEEEEECHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]