Click here to switch to the map view.

The map label for this gene is pflC

Identifier: 30064761

GI number: 30064761

Start: 3611015

End: 3611893

Strand: Reverse

Name: pflC

Synonym: S3717

Alternate gene names: 30064761

Gene position: 3611893-3611015 (Counterclockwise)

Preceding gene: 30064762

Following gene: 30064760

Centisome position: 78.53

GC content: 54.84

Gene sequence:

>879_bases
ATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACAT
TCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGT
GTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTG
CGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGT
GATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGT
TTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTCTCATGCGCCATTGAAACTGCCGGAGACGCGCCAGCAAGC
AAGCTGTTACCGCTGGCGAAATTGTGCGATGAAGTGCTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGT
GGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGTGTCAACGTGATCCCGCGTTTAC
CGTTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAAAATCAGGCAG
ATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCC
TGCGCCGTCGTCAGCCGATGTGACAACGATGCGCGAAATGGCAGAACGGGCCGGATTTCAGGTTACCGTGGGAGGTTAA

Upstream 100 bases:

>100_bases
TTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGA
ACTGTCGAAGGAGATCCAGG

Downstream 100 bases:

>100_bases
AATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGT
TGTGCCAGTTAGAGAAGTGG

Product: pyruvate formate lyase II activase

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCL
RDADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQ
IHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG

Sequences:

>Translated_292_residues
MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCL
RDADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQ
IHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG
>Mature_291_residues
TSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLR
DADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPASK
LLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQI
HLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=292, Percent_Identity=98.972602739726, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=34.4481605351171, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787130, Length=274, Percent_Identity=25.5474452554745, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790839, Length=254, Percent_Identity=23.2283464566929, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 32508; Mature: 32377

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESI
CCCCCCCEEEEEEECCCHHHHHHHHHEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHC
SGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCC
VTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVLFDLKIMD
EEEECCCEEEEHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
ATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEE
IHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG
EEEEECHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESI
CCCCCCEEEEEEECCCHHHHHHHHHEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHC
SGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCC
VTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVLFDLKIMD
EEEECCCEEEEHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
ATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEE
IHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG
EEEEECHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]