Click here to switch to the map view.

The map label for this gene is pflD

Identifier: 30064762

GI number: 30064762

Start: 3611859

End: 3614156

Strand: Reverse

Name: pflD

Synonym: S3718

Alternate gene names: 30064762

Gene position: 3614156-3611859 (Counterclockwise)

Preceding gene: 30064763

Following gene: 30064761

Centisome position: 78.58

GC content: 52.87

Gene sequence:

>2298_bases
ATGACGAATCGTATCTCTCGCCTCAAAACTGCACTGTTTGCCAATACCCGTGAAATCTCGCTGGAGCGGGCGCTGCTTTA
TACCGCCAGCCATCGGCAAACCGAAGGCGAACCGGTGATATTGCGCCGGGCGAAAGCAACAGCGTATATCCTTGAACATG
TTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAA
ATGGACCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGA
TAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAG
ATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGAT
TACCCACGCCTGTTGAATCACGGTCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCA
CTTTTATCAGGCCGCGTTGTTACTGCTGGAAGCCTCGCAGAAACACATTTTGCGTTACGCCGAACTGGCGGAAACGATGG
CGGCAAACTGCACAGATGCCCAGCGTCGCGAAGAGCTGCTGACTATTGCGGAGATCTCCCGCCATAACGCGCAACATAAG
CCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCT
GTCTTTAGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGGCATCTTTAACCCAGGGCGAAGATCCGGCGTTCCTGA
AAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCC
GGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCT
TTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGT
TCCTGATGAAAACCGCCGAAACCATTCGCCTCGGCACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCG
TTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG
AACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATG
CTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGC
AATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCGTCTTTTATCAGCGATTGTCTGGAAAAAGG
TCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGAATTGGTATCGCCAACCTGAGCGATTCTC
TCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTT
GCAACGCCAGAAGGCGAAAAAGTCCGCGCTCGCTTAATTAACCGCTTTGAGAAATACGGTAACGATATCGACGAGGTGGA
TAACATTAGCGCCGAACTGTTGCGCCACTACTGCAAAGAAGTGGAAAAATACCAGAACCCGCGCGGCGGTTACTTCACGC
CGGGATCGTATACCGTTTCTGCTCACGTTCCGTTGGGATCGGTGGTTGGCGCGACGCCAGACGGTCGTTTTGCCGGGGAA
CAACTGGCAGACGGCGGCTTGTCACCTATGCTGGGTCAGGACGCACAAGGGCCAACGGCGGTACTGAAGTCAGTCAGTAA
GCTCGATAATACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCGGGAT
TACGCAAACTGGCCGACTTCTTACGGGTGTTTACCCAGCTTAAGTTGCAGCATATTCAGTTTAACGTGGTGAACGCCGAC
ACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGT
CGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAA

Upstream 100 bases:

>100_bases
CTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCG
CTTTCCCACAGGAGTTCCTC

Downstream 100 bases:

>100_bases
CGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTC
GTACGGTGGTCTTTTTTAAA

Product: putative formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Sequences:

>Translated_765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
>Mature_764_residues
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEM
DPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIIDY
PRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHKP
QTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAG
FPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAF
LNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGSN
ICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFA
TPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQ
LADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADT
LREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=99.4771241830065, Blast_Score=1583, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=805, Percent_Identity=37.3913043478261, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI48994926, Length=641, Percent_Identity=25.585023400936, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1787131, Length=543, Percent_Identity=24.8618784530387, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85951; Mature: 85820

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRD
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
VFEQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
VFEQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]