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The map label for this gene is yhdG

Identifier: 30064591

GI number: 30064591

Start: 3388057

End: 3388899

Strand: Direct

Name: yhdG

Synonym: S3515

Alternate gene names: 30064591

Gene position: 3388057-3388899 (Clockwise)

Preceding gene: 30064590

Following gene: 30064592

Centisome position: 73.66

GC content: 52.08

Gene sequence:

>843_bases
ATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC
CGTGCAAATTGCTGGTAGCGCTCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTG
ATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTT
AAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACA
CCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCT
GTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGC
GACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCA
GGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCACTGCCTTTGGCAGAGG
TTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAA
CACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATGACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGA
ACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAA

Upstream 100 bases:

>100_bases
CAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCG
GATTGACAGTATCCGAGATG

Downstream 100 bases:

>100_bases
ACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTC
AGGATCAGGTAACCCAAAAA

Product: putative dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDVV
KSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDCGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANG
DITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARK
HVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA

Sequences:

>Translated_280_residues
MSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDVV
KSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDCGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANG
DITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARK
HVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA
>Mature_279_residues
SSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDVVK
SILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDCGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGD
ITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARKH
VSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=261, Percent_Identity=30.6513409961686, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI239788483, Length=175, Percent_Identity=32, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI239788462, Length=178, Percent_Identity=32.5842696629214, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI8923374, Length=172, Percent_Identity=34.8837209302326, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=175, Percent_Identity=32.5714285714286, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789660, Length=280, Percent_Identity=99.6428571428571, Blast_Score=579, Evalue=1e-167,
Organism=Escherichia coli, GI1788462, Length=254, Percent_Identity=31.1023622047244, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=199, Percent_Identity=33.1658291457286, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=179, Percent_Identity=35.7541899441341, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=183, Percent_Identity=33.879781420765, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=195, Percent_Identity=30.2564102564103, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=208, Percent_Identity=31.25, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=149, Percent_Identity=32.2147651006711, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585320, Length=177, Percent_Identity=32.7683615819209, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45549423, Length=147, Percent_Identity=39.4557823129252, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24580595, Length=187, Percent_Identity=31.5508021390374, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19920448, Length=187, Percent_Identity=31.5508021390374, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921524, Length=177, Percent_Identity=28.8135593220339, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 31208; Mature: 31077

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCP
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHEECCCCCEEEEECCCCC
AKKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHC
GIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADAL
CCEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHEEEEECCCCEE
MIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARK
EECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCP
CCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHEECCCCCEEEEECCCCC
AKKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHC
GIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADAL
CCEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHEEEEECCCCEE
MIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARK
EECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]