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The map label for this gene is mtr

Identifier: 30064501

GI number: 30064501

Start: 3287896

End: 3289140

Strand: Reverse

Name: mtr

Synonym: S3419

Alternate gene names: 30064501

Gene position: 3289140-3287896 (Counterclockwise)

Preceding gene: 161486429

Following gene: 30064497

Centisome position: 71.51

GC content: 53.49

Gene sequence:

>1245_bases
ATGGCAACACTAACCACCACCCAAACGTCACCGTCGCTGCTTGGCGGCGTGGTGATTATCGGCGGCACCATTATTGGCGC
AGGGATGTTTTCTCTGCCAGTGGTCATGTCCGGGGCGTGGTTTTTCTGGTCAATGGCAGCGCTGATCTTTACCTGGTTCT
GTATGCTGCATTCCGGCTTGATGATTCTGGAAGCTAACCTGAATTACCGAATCGGTTCGAGTTTTGACACCATCACCAAA
GATTTGCTGGGCAAAGGCTGGAACGTGGTCAACGGCATTTCCATTGCCTTTGTGCTCTATATCCTGACCTATGCCTATAT
TTCTGCCAGTGGTTCGATTCTGCATCACACCTTCGCAGAGATGTCGCTAAACGTCCCGGCACGGGCGGCGGGTTTTGGTT
TTGCACTGCTGGTAGCGTTTGTGGTGTGGTTGAGTACTAAAGCCGTCAGCCGCATGACGGCGATTGTGCTGGGGGCGAAA
GTCATTACCTTCTTCCTCACCTTCGGTAGCCTGCTGGGGCATGTGCAGCCTGCGACATTGTTCAACGTTGCCGAAAGCAA
TGCATCTTATGCGCCGTATTTGCTGATGACCCTGCCGTTCTGCCTGGCGTCGTTTGGTTATCACGGTAACGTGCCAAGCC
TGATGAAGTATTACGGCAAAGATCCGAAAACCATCGTGAAATGCCTGGTGTACGGTACGCTGATGGCACTGGCGCTGTAT
ACCATCTGGTTGCTGGCGACAATGGGCAACATCCCTCGTCCGGAGTTTATCGGCATCGCCGAGAAGGGCGGTAATATTGA
TGTGTTGGTACAAGCATTAAGCGGCGTGCTGAACAGCCGTAGCCTGGACCTTCTGCTGGTCGTGTTCTCAAACTTTGCGG
TAGCGAGTTCGTTCCTTGGCGTAACGCTGGGTTTGTTTGACTATCTGGCAGATCTGTTTGGTTTCGACGACTCGGCTGTG
GGCCGCTTGAAAACGGCATTGCTGACCTTTGCCCCGCCTGTTGTGGGTGGTCTGCTGTTCCCGAACGGATTCCTGTATGC
CATTGGTTATGCTGGTTTAGCGGCTACCATCTGGGCGGCAATTGTTCCGGCACTGCTGGCCCGCGCATCGCGTAAACGCT
TTGGCAGCCCGAAATTCCGCGTCTGGGGTGGCAAGCCGATGATTGCGCTGATTCTGGTGTTTGGCGTCGGCAACGCACTG
GTGCATATTTTATCGAGCTTTAATTTACTGCCGGTGTATCAGTAA

Upstream 100 bases:

>100_bases
TAACCATCGGGGCTTTTTTTCTGTCTTTTGTACTCATGTACTGGTACAGTGCAATGCATAACAACGCAGTCGCACTATTT
TTCACTGGAGAGAAGCCCTC

Downstream 100 bases:

>100_bases
TCAGCGGTGCCTTATCCGACATTTCTGCTGCCTACACAATGCCTGATGCGCTTCGCTTATCAGGCCTACGTAGGACAGCG
TTGCCAGCTCGGATAAGGCT

Product: tryptophan permease

Products: Indole [Cytoplasm]; L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Tryptophan permease

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSMAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITK
DLLGKGWNVVNGISIAFVLYILTYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPKTIVKCLVYGTLMALALY
TIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAV
GRLKTALLTFAPPVVGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALILVFGVGNAL
VHILSSFNLLPVYQ

Sequences:

>Translated_414_residues
MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSMAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITK
DLLGKGWNVVNGISIAFVLYILTYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPKTIVKCLVYGTLMALALY
TIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAV
GRLKTALLTFAPPVVGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALILVFGVGNAL
VHILSSFNLLPVYQ
>Mature_413_residues
ATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSMAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKD
LLGKGWNVVNGISIAFVLYILTYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAKV
ITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPKTIVKCLVYGTLMALALYT
IWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVG
RLKTALLTFAPPVVGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALILVFGVGNALV
HILSSFNLLPVYQ

Specific function: Involved in transporting tryptophan across the cytoplasmic membrane

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily

Homologues:

Organism=Escherichia coli, GI1789552, Length=414, Percent_Identity=100, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI1790145, Length=409, Percent_Identity=52.8117359413203, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1788218, Length=399, Percent_Identity=33.5839598997494, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTR_ECOLI (P0AAD2)

Other databases:

- EMBL:   M59862
- EMBL:   M58338
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A39187
- RefSeq:   AP_003706.1
- RefSeq:   NP_417630.1
- ProteinModelPortal:   P0AAD2
- STRING:   P0AAD2
- PRIDE:   P0AAD2
- EnsemblBacteria:   EBESCT00000004582
- EnsemblBacteria:   EBESCT00000004583
- EnsemblBacteria:   EBESCT00000014310
- GeneID:   947675
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3130
- KEGG:   eco:b3161
- EchoBASE:   EB0612
- EcoGene:   EG10617
- eggNOG:   COG0814
- GeneTree:   EBGT00050000009663
- HOGENOM:   HBG482321
- OMA:   WPNGFLY
- ProtClustDB:   PRK10483
- BioCyc:   EcoCyc:MTR-MONOMER
- Genevestigator:   P0AAD2
- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227
- PRINTS:   PR00166
- TIGRFAMs:   TIGR00837

Pfam domain/function: PF03222 Trp_Tyr_perm

EC number: NA

Molecular weight: Translated: 44333; Mature: 44202

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00594 AROMATIC_AA_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x142f0384)-; HASH(0x13cdffc8)-; HASH(0x14d1cbb8)-; HASH(0x140c4388)-; HASH(0x14e26b2c)-; HASH(0x15321174)-; HASH(0x14f073b0)-; HASH(0x14f11bb0)-; HASH(0x153bb910)-; HASH(0x153c1588)-; HASH(0x1533909c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSMAALIFTWFCMLHSGL
CCCEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYILTYAYISASGSILHHTFAE
EEEEECCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAKVITFFLTFGSLLGHVQPATL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPKTIVKCLVYGTLMALALY
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLG
HHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPVVGGLLFPNGFLYAIGYAGLAATIWAA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IVPALLARASRKRFGSPKFRVWGGKPMIALILVFGVGNALVHILSSFNLLPVYQ
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
ATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSMAALIFTWFCMLHSGL
CCEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYILTYAYISASGSILHHTFAE
EEEEECCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAKVITFFLTFGSLLGHVQPATL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPKTIVKCLVYGTLMALALY
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLG
HHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPVVGGLLFPNGFLYAIGYAGLAATIWAA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IVPALLARASRKRFGSPKFRVWGGKPMIALILVFGVGNALVHILSSFNLLPVYQ
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Indole [Periplasm]; L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: Proton [Periplasm] + indole [Periplasm] = Proton [Cytoplasm] + indole [Cytoplasm] L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1987112; 2061290; 9278503; 1904443; 7814318