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The map label for this gene is insB1 [H]

Identifier: 30064480

GI number: 30064480

Start: 3268746

End: 3269249

Strand: Reverse

Name: insB1 [H]

Synonym: S3394

Alternate gene names: 30064480

Gene position: 3269249-3268746 (Counterclockwise)

Preceding gene: 30064481

Following gene: 30064468

Centisome position: 71.08

GC content: 52.58

Gene sequence:

>504_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACATTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGTTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGAC
AACATCTGGCAAGGCTGGGACGGAAGTTACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTAT
CTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCGCCATCAAAAACTATTTCACCATCGGTTGCAGCGGCATTACCTGCGAAAAGCGCAAGTAAGCCCA
CAATAGCTGTTTTTGTAACT

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKLLSFSKSVELHDKVIGHY
LNIKHYQ

Sequences:

>Translated_167_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKLLSFSKSVELHDKVIGHY
LNIKHYQ
>Mature_166_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKLLSFSKSVELHDKVIGHYL
NIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=167, Percent_Identity=89.8203592814371, Blast_Score=314, Evalue=2e-87,
Organism=Escherichia coli, GI1788203, Length=167, Percent_Identity=89.8203592814371, Blast_Score=314, Evalue=2e-87,
Organism=Escherichia coli, GI1786203, Length=167, Percent_Identity=89.8203592814371, Blast_Score=314, Evalue=2e-87,
Organism=Escherichia coli, GI1787223, Length=167, Percent_Identity=88.0239520958084, Blast_Score=310, Evalue=5e-86,
Organism=Escherichia coli, GI1786470, Length=167, Percent_Identity=88.0239520958084, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI1786459, Length=167, Percent_Identity=88.0239520958084, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 19772; Mature: 19641

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKLLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKLLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]