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The map label for this gene is tdcA

Identifier: 30064461

GI number: 30064461

Start: 3248751

End: 3249689

Strand: Reverse

Name: tdcA

Synonym: S3370

Alternate gene names: 30064461

Gene position: 3249689-3248751 (Counterclockwise)

Preceding gene: 161486432

Following gene: 30064460

Centisome position: 70.66

GC content: 42.17

Gene sequence:

>939_bases
ATGAGCACTATTCTTCTTCCGAAAACGCAGCACCTGGTAGTCTTTCAGGAAGTCATTAGAAGTGGCTCTATCGGCTCGGC
TGCAAAAGAATTAGGGTTAACTCAACCGGCCGTCAGTAAAATCATTAACGATATTGAAGATTATTTTGGTGTGGAATTAG
TGGTGCGGAAAAATACCGGTGTAACATTAACACCTGCCGGTCAATTGTTACTCTCCCGTTCCGAATCCATTACCCGTGAA
ATGAAAAATATGGTTAATGAGATAAGCGGTATGTCTTCTGAAGCGGTGGTGGAAGTCTCATTTGGTTTTCCTTCATTGAT
TGGTTTTACTTTTATGTCAGGGATGATCAACAAGTTCAAAGAGGTGTTCCCGAAAGCGCAGGTTTCTATGTATGAAGCGC
AACTGTCTTCGTTCTTACCGGCAATCCGCGACGGTCGACTGGATTTTGCGATTGGTACGTTAAGTGCAGAAATGAAGCTT
CAGGATTTACATGTTGAGCCGCTGTTCGAGTCCGAGTTTGTGCTGGTAGCCAGTAAGTCCCGAACATGCACCGGCACCAC
CACGCTGGAGTCGTTGAAGAACGAACAGTGGGTGTTGCCACAAACTAATATGGGGTACTACAGCGAACTGCTTACTACGT
TACAAAGAAATGGCATCAGTATTGAAAACATCGTTAAAACCGACTCAGTCGTGACAATTTATAATCTTGTTCTCAATGCT
GATTTCTTAACTGTAATTCCTTGTGATATGACGTCACCTTTTGGTTCTAATCAATTTATTACTATTCCGGTTGAAGAAAC
ATTACCTGTGGCACAATATGCCGCGGTATGGTCGAAAAATTATCGTATTAAAAAAGCAGCATCGGTTTTGGTGGAATTAG
CCAAAGAGTATTCATCTTATAATGGGTGTAGACGAAGGCAATTAATAGAAGTTGGTTAG

Upstream 100 bases:

>100_bases
GGAAAGTAATGAAAGTTAATTTGTGAGTGGTCGCACATATCCTGTTTATTTCATTTTGATACACTTCATGCCGTCAATGA
GGTAATTAACGTAGGTCGTT

Downstream 100 bases:

>100_bases
TTGTTTGTTTTTATTTAAACATAAATAATCCACCTGTCTGTTTTGCAGGTGTCGGTTACGGTTACCTACATATTTAATTC
AGGCGAAGAGGTTTTATAAT

Product: DNA-binding transcriptional activator TdcA

Products: NA

Alternate protein names: Tdc operon transcriptional activator

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITRE
MKNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNA
DFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG

Sequences:

>Translated_312_residues
MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITRE
MKNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNA
DFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG
>Mature_311_residues
STILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREM
KNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKLQ
DLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNAD
FLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG

Specific function: Transcriptional activator for the tdcABCDE operon

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789506, Length=312, Percent_Identity=100, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1787601, Length=291, Percent_Identity=27.1477663230241, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788481, Length=197, Percent_Identity=27.4111675126904, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=22.887323943662, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=238, Percent_Identity=25.2100840336134, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=23.6301369863014, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=25.9786476868327, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790262, Length=190, Percent_Identity=24.2105263157895, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI145693105, Length=161, Percent_Identity=27.3291925465839, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TDCA_ECO57 (P0ACQ8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85973
- PIR:   F91128
- RefSeq:   NP_289690.1
- RefSeq:   NP_312025.1
- ProteinModelPortal:   P0ACQ8
- SMR:   P0ACQ8
- EnsemblBacteria:   EBESCT00000028941
- EnsemblBacteria:   EBESCT00000057298
- GeneID:   916165
- GeneID:   961446
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4470
- KEGG:   ecs:ECs3998
- GeneTree:   EBGT00050000008328
- HOGENOM:   HBG417057
- OMA:   DEMKLQD
- ProtClustDB:   PRK10341
- BioCyc:   ECOL83334:ECS3998-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34539; Mature: 34408

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTG
CCCEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCC
VTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFK
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
EVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKLQDLHVEPLFESEFVLVASKS
HHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHEEHEECCCCCCCCCCEEEEEECC
RTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNA
CCCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCC
DFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSY
CEEEEEECCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
NGCRRRQLIEVG
CCHHHHHHCCCC
>Mature Secondary Structure 
STILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTG
CCEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCC
VTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFK
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
EVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKLQDLHVEPLFESEFVLVASKS
HHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHEEHEECCCCCCCCCCEEEEEECC
RTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNA
CCCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCC
DFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSY
CEEEEEECCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
NGCRRRQLIEVG
CCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796