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The map label for this gene is tdcB

Identifier: 30064460

GI number: 30064460

Start: 3247663

End: 3248652

Strand: Reverse

Name: tdcB

Synonym: S3369

Alternate gene names: 30064460

Gene position: 3248652-3247663 (Counterclockwise)

Preceding gene: 30064461

Following gene: 30064459

Centisome position: 70.63

GC content: 46.06

Gene sequence:

>990_bases
ATGCATATTACATACGATCTGCCGGTTGCTATTGATGATATTATTGAAGCGAAACAACGACTGGCTGGGCGAATTTATAA
AACAGGCATGCCTCGCTCCAACTATTTTAGTGAACGTTGCAAAGGTGAAATATTCCTGAAGTTTGAAAATATGCAGCGTA
CGGGTTCATTTAAAATTCGTGGCGCATTTAATAAATTAAGTTCACTGACCGATGCGGAAAAACGCAAAGGCGTGGTGGCC
TGTTCTGCGGGCAACCATGCGCAAGGGGTTTCGCTCTCCTGCGCGATGCTGGGTATCGACGGTAAAGTGGTGATGCCAAA
AGGTGCGCCAAAATCCAAAGTAGCGGCAACGTGCGACTACTCCGCAGAAGTCGTTCTGCATGGTGATAACTTCAACGACA
CTATCGCTAAAGTGAGCGAAATTGTCGAAATGGAAGGTCGTATTTTTATCCCACCTTACGATGATCCGAAAGTGATTGCT
GGCCAGGGAACGATTGGTCTGGAAATTATGGAAGATCTCTATGATGTCGATAACGTGATTGTGCCAATTGGTGGTGGTGG
TTTAATTGCTGGTATTGCGGTGGCAATTAAATCTATTAACCCGACCATTCGTGTTATTGGCGTACAGTCAGAAAACGTTC
ACGGCATGGCGGCTTCTTTCCACTCCGGAGAAATAACCACGCACCGAACTACCGGCACCCTGGCGGATGGTTGTGATGTC
TCCCGCCCGGGTAATTTAACTTACGAAATCGTTCGTGAATTAGTCGATGACATCGTGCTGGTCAGCGAAGACGAAATCAG
AAACAGTATGATTGCCTTAATTCAGCGCAATAAAGTCGTCACCGAAGGCGCAGGCGCTCTGGCATGTGCTGCATTATTAA
GCGGTAAATTAGACCAATATATTCAAAACAGAAAAACCGTCAGTATTATTTCCGGCGGCAATATCGATCTTTCTCGCGTC
TCTCAAATCACCGGTTTCGTTGACGCTTAA

Upstream 100 bases:

>100_bases
AGTTGTTTGTTTTTATTTAAACATAAATAATCCACCTGTCTGTTTTGCAGGTGTCGGTTACGGTTACCTACATATTTAAT
TCAGGCGAAGAGGTTTTATA

Downstream 100 bases:

>100_bases
TTAATTCGTTGAGGATAGGATATGAGTACTTCAGATAGCATTGTATCCAGCCAGACAAAACAATCGTCCTGGCGTAAATC
AGATACCACATGGACGTTAG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEIFLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVA
CSAGNHAQGVSLSCAMLGIDGKVVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAASFHSGEITTHRTTGTLADGCDV
SRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRV
SQITGFVDA

Sequences:

>Translated_329_residues
MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEIFLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVA
CSAGNHAQGVSLSCAMLGIDGKVVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAASFHSGEITTHRTTGTLADGCDV
SRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRV
SQITGFVDA
>Mature_329_residues
MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEIFLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVA
CSAGNHAQGVSLSCAMLGIDGKVVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAASFHSGEITTHRTTGTLADGCDV
SRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRV
SQITGFVDA

Specific function: Acts on both serine and threonine, and properly considered as a hydroxy amino acid deaminase

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family

Homologues:

Organism=Homo sapiens, GI11345492, Length=318, Percent_Identity=36.4779874213836, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1789505, Length=329, Percent_Identity=100, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1790207, Length=297, Percent_Identity=37.7104377104377, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71991565, Length=316, Percent_Identity=38.2911392405063, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17537387, Length=304, Percent_Identity=39.4736842105263, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17508781, Length=317, Percent_Identity=29.6529968454259, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322631, Length=311, Percent_Identity=39.2282958199357, Blast_Score=205, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6320930, Length=296, Percent_Identity=35.472972972973, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6319788, Length=281, Percent_Identity=25.2669039145907, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355833, Length=310, Percent_Identity=38.3870967741935, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24645328, Length=214, Percent_Identity=38.3177570093458, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THD2_ECO57 (P0AGF8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85973
- PIR:   E91128
- RefSeq:   NP_289689.1
- RefSeq:   NP_312024.1
- ProteinModelPortal:   P0AGF8
- SMR:   P0AGF8
- EnsemblBacteria:   EBESCT00000026695
- EnsemblBacteria:   EBESCT00000058964
- GeneID:   916167
- GeneID:   958586
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4469
- KEGG:   ecs:ECs3997
- GeneTree:   EBGT00050000009483
- HOGENOM:   HBG714501
- OMA:   VEHYEAP
- ProtClustDB:   PRK08638
- BioCyc:   ECOL83334:ECS3997-MONOMER
- InterPro:   IPR001926
- InterPro:   IPR000634

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.3.1.19

Molecular weight: Translated: 35233; Mature: 35233

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEIFLKFENMQRTGSFKIR
CEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCEEEE
GAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGKVVMPKGAPKSKVAATCDY
EHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEECCCEEEECCCCCCCCEEEEECC
SAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIAGQGTIGLEIMEDLYDVDNVI
CEEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEECCCCCHHHHHHHHHCCCCEE
VPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAASFHSGEITTHRTTGTLADGCDV
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
SRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVVTEGAGALACAALLSGKLDQY
CCCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
IQNRKTVSIISGGNIDLSRVSQITGFVDA
HHCCCEEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEIFLKFENMQRTGSFKIR
CEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCEEEE
GAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGKVVMPKGAPKSKVAATCDY
EHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEECCCEEEECCCCCCCCEEEEECC
SAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIAGQGTIGLEIMEDLYDVDNVI
CEEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEECCCCCHHHHHHHHHCCCCEE
VPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAASFHSGEITTHRTTGTLADGCDV
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
SRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVVTEGAGALACAALLSGKLDQY
CCCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
IQNRKTVSIISGGNIDLSRVSQITGFVDA
HHCCCEEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796